Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 16 nucleotides fruK and fruB overlap by 1 nucleotides fruB and setB are separated by 367 nucleotides
b2167: fruA - fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI), at 2,257,741 to 2,259,432
fruA
b2168: fruK - 1-phosphofructokinase (NCBI), at 2,259,449 to 2,260,387
fruK
b2169: fruB - fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI), at 2,260,387 to 2,261,517
fruB
b2170: setB - lactose/glucose efflux system (NCBI), at 2,261,885 to 2,263,066
setB
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2259.507 kb on + strand at 2259.951 kb on - strand, within fruK at 2260.134 kb on + strand, within fruK at 2260.153 kb on + strand, within fruK at 2260.398 kb on + strand at 2260.398 kb on + strand at 2260.412 kb on - strand at 2260.416 kb on - strand at 2260.592 kb on + strand, within fruB at 2260.635 kb on - strand, within fruB at 2260.821 kb on - strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.938 kb on + strand, within fruB at 2261.006 kb on - strand, within fruB at 2261.202 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.255 kb on + strand, within fruB at 2261.330 kb on - strand, within fruB at 2261.476 kb on - strand at 2261.937 kb on - strand at 2262.160 kb on - strand, within setB at 2262.373 kb on - strand, within setB
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 2,259,507 + +0.1 2,259,951 - fruK b2168 0.53 -1.9 2,260,134 + fruK b2168 0.73 -0.4 2,260,153 + fruK b2168 0.75 +1.2 2,260,398 + +0.2 2,260,398 + +0.9 2,260,412 - -0.9 2,260,416 - -1.0 2,260,592 + fruB b2169 0.18 +0.1 2,260,635 - fruB b2169 0.22 +0.3 2,260,821 - fruB b2169 0.38 +0.4 2,260,911 + fruB b2169 0.46 +0.1 2,260,911 + fruB b2169 0.46 +0.2 2,260,938 + fruB b2169 0.49 +0.3 2,261,006 - fruB b2169 0.55 +1.7 2,261,202 + fruB b2169 0.72 -0.8 2,261,241 + fruB b2169 0.76 +0.8 2,261,241 + fruB b2169 0.76 +2.2 2,261,255 + fruB b2169 0.77 +2.2 2,261,330 - fruB b2169 0.83 -0.2 2,261,476 - +2.3 2,261,937 - +1.0 2,262,160 - setB b2170 0.23 +0.7 2,262,373 - setB b2170 0.41 +0.8
Or see this region's nucleotide sequence