Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 16 nucleotides fruK and fruB overlap by 1 nucleotides
b2167: fruA - fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI), at 2,257,741 to 2,259,432
fruA
b2168: fruK - 1-phosphofructokinase (NCBI), at 2,259,449 to 2,260,387
fruK
b2169: fruB - fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI), at 2,260,387 to 2,261,517
fruB
Position (kb)
2259
2260
2261 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2258.450 kb on + strand, within fruA at 2258.529 kb on - strand, within fruA at 2258.529 kb on - strand, within fruA at 2258.661 kb on + strand, within fruA at 2258.756 kb on - strand, within fruA at 2258.760 kb on - strand, within fruA at 2258.845 kb on - strand, within fruA at 2258.893 kb on - strand, within fruA at 2258.970 kb on + strand, within fruA at 2259.012 kb on + strand, within fruA at 2259.089 kb on - strand, within fruA at 2259.089 kb on - strand, within fruA at 2259.094 kb on - strand, within fruA at 2259.106 kb on + strand, within fruA at 2259.184 kb on + strand, within fruA at 2259.184 kb on + strand, within fruA at 2259.197 kb on + strand, within fruA at 2259.215 kb on - strand, within fruA at 2259.380 kb on + strand at 2259.507 kb on + strand at 2259.951 kb on - strand, within fruK at 2260.134 kb on + strand, within fruK at 2260.153 kb on + strand, within fruK at 2260.398 kb on + strand at 2260.398 kb on + strand at 2260.412 kb on - strand at 2260.416 kb on - strand at 2260.592 kb on + strand, within fruB at 2260.635 kb on - strand, within fruB at 2260.821 kb on - strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.938 kb on + strand, within fruB at 2261.006 kb on - strand, within fruB at 2261.202 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.255 kb on + strand, within fruB at 2261.330 kb on - strand, within fruB
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 2,258,450 + fruA b2167 0.42 +0.8 2,258,529 - fruA b2167 0.47 -0.4 2,258,529 - fruA b2167 0.47 -0.1 2,258,661 + fruA b2167 0.54 -0.3 2,258,756 - fruA b2167 0.60 -0.6 2,258,760 - fruA b2167 0.60 +0.6 2,258,845 - fruA b2167 0.65 -1.9 2,258,893 - fruA b2167 0.68 +0.2 2,258,970 + fruA b2167 0.73 -0.4 2,259,012 + fruA b2167 0.75 +0.8 2,259,089 - fruA b2167 0.80 +0.2 2,259,089 - fruA b2167 0.80 +0.5 2,259,094 - fruA b2167 0.80 +0.4 2,259,106 + fruA b2167 0.81 -0.5 2,259,184 + fruA b2167 0.85 +1.0 2,259,184 + fruA b2167 0.85 +1.2 2,259,197 + fruA b2167 0.86 -1.4 2,259,215 - fruA b2167 0.87 -0.6 2,259,380 + +0.4 2,259,507 + +0.1 2,259,951 - fruK b2168 0.53 -1.9 2,260,134 + fruK b2168 0.73 -0.4 2,260,153 + fruK b2168 0.75 +1.2 2,260,398 + +0.2 2,260,398 + +0.9 2,260,412 - -0.9 2,260,416 - -1.0 2,260,592 + fruB b2169 0.18 +0.1 2,260,635 - fruB b2169 0.22 +0.3 2,260,821 - fruB b2169 0.38 +0.4 2,260,911 + fruB b2169 0.46 +0.1 2,260,911 + fruB b2169 0.46 +0.2 2,260,938 + fruB b2169 0.49 +0.3 2,261,006 - fruB b2169 0.55 +1.7 2,261,202 + fruB b2169 0.72 -0.8 2,261,241 + fruB b2169 0.76 +0.8 2,261,241 + fruB b2169 0.76 +2.2 2,261,255 + fruB b2169 0.77 +2.2 2,261,330 - fruB b2169 0.83 -0.2
Or see this region's nucleotide sequence