Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ychQ and ychA are separated by 3 nucleotides ychA and kdsA are separated by 35 nucleotides kdsA and ldrA are separated by 148 nucleotides ldrA and rdlA are separated by 47 nucleotides rdlA and ldrB are separated by 313 nucleotides ldrB and rdlB are separated by 47 nucleotides
b1213: ychQ - predicted transcriptional regulator (NCBI), at 1,266,147 to 1,266,539
ychQ
b1214: ychA - predicted transcriptional regulator (NCBI), at 1,266,543 to 1,267,352
ychA
b1215: kdsA - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI), at 1,267,388 to 1,268,242
kdsA
b4419: ldrA - toxic polypeptide, small (NCBI), at 1,268,391 to 1,268,498
ldrA
b4420: rdlA - regulatory antisense RNA (NCBI), at 1,268,546 to 1,268,612
rdlA
b4421: ldrB - toxic polypeptide, small (NCBI), at 1,268,926 to 1,269,033
ldrB
b4422: rdlB - regulatory antisense sRNA (NCBI), at 1,269,081 to 1,269,146
rdlB
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-2
-1
0
1 at 1266.435 kb on - strand, within ychQ at 1266.483 kb on + strand, within ychQ at 1266.522 kb on - strand at 1266.644 kb on - strand, within ychA at 1266.701 kb on + strand, within ychA at 1266.737 kb on + strand, within ychA at 1266.990 kb on + strand, within ychA at 1267.094 kb on + strand, within ychA at 1267.104 kb on - strand, within ychA at 1267.139 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.242 kb on + strand, within ychA at 1267.274 kb on + strand at 1267.274 kb on + strand at 1267.274 kb on - strand at 1267.276 kb on + strand at 1267.276 kb on + strand at 1268.787 kb on + strand at 1268.787 kb on + strand at 1269.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 1,266,435 - ychQ b1213 0.73 -2.4 1,266,483 + ychQ b1213 0.85 -0.4 1,266,522 - -0.1 1,266,644 - ychA b1214 0.12 -0.4 1,266,701 + ychA b1214 0.20 -1.2 1,266,737 + ychA b1214 0.24 -1.9 1,266,990 + ychA b1214 0.55 +0.1 1,267,094 + ychA b1214 0.68 -0.4 1,267,104 - ychA b1214 0.69 -2.0 1,267,139 + ychA b1214 0.74 -1.7 1,267,242 + ychA b1214 0.86 +0.8 1,267,242 + ychA b1214 0.86 -0.3 1,267,242 + ychA b1214 0.86 +0.1 1,267,274 + -1.7 1,267,274 + -0.4 1,267,274 - -0.9 1,267,276 + -1.2 1,267,276 + +0.1 1,268,787 + +0.3 1,268,787 + +1.6 1,269,230 + +0.2
Or see this region's nucleotide sequence