Strain Fitness in Escherichia coli BW25113 around b1215

Experiment: Bacitracin 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntychQ and ychA are separated by 3 nucleotidesychA and kdsA are separated by 35 nucleotideskdsA and ldrA are separated by 148 nucleotidesldrA and rdlA are separated by 47 nucleotidesrdlA and ldrB are separated by 313 nucleotidesldrB and rdlB are separated by 47 nucleotides b1213: ychQ - predicted transcriptional regulator (NCBI), at 1,266,147 to 1,266,539 ychQ b1214: ychA - predicted transcriptional regulator (NCBI), at 1,266,543 to 1,267,352 ychA b1215: kdsA - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI), at 1,267,388 to 1,268,242 kdsA b4419: ldrA - toxic polypeptide, small (NCBI), at 1,268,391 to 1,268,498 ldrA b4420: rdlA - regulatory antisense RNA (NCBI), at 1,268,546 to 1,268,612 rdlA b4421: ldrB - toxic polypeptide, small (NCBI), at 1,268,926 to 1,269,033 ldrB b4422: rdlB - regulatory antisense sRNA (NCBI), at 1,269,081 to 1,269,146 rdlB Position (kb) 1267 1268 1269Strain fitness (log2 ratio) -2 -1 0 1at 1266.435 kb on - strand, within ychQat 1266.483 kb on + strand, within ychQat 1266.522 kb on - strandat 1266.644 kb on - strand, within ychAat 1266.701 kb on + strand, within ychAat 1266.737 kb on + strand, within ychAat 1266.990 kb on + strand, within ychAat 1267.094 kb on + strand, within ychAat 1267.104 kb on - strand, within ychAat 1267.139 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.274 kb on + strandat 1267.274 kb on + strandat 1267.274 kb on - strandat 1267.276 kb on + strandat 1267.276 kb on + strandat 1268.787 kb on + strandat 1268.787 kb on + strandat 1269.230 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml
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1,266,435 - ychQ b1213 0.73 -2.4
1,266,483 + ychQ b1213 0.85 -0.4
1,266,522 - -0.1
1,266,644 - ychA b1214 0.12 -0.4
1,266,701 + ychA b1214 0.20 -1.2
1,266,737 + ychA b1214 0.24 -1.9
1,266,990 + ychA b1214 0.55 +0.1
1,267,094 + ychA b1214 0.68 -0.4
1,267,104 - ychA b1214 0.69 -2.0
1,267,139 + ychA b1214 0.74 -1.7
1,267,242 + ychA b1214 0.86 +0.8
1,267,242 + ychA b1214 0.86 -0.3
1,267,242 + ychA b1214 0.86 +0.1
1,267,274 + -1.7
1,267,274 + -0.4
1,267,274 - -0.9
1,267,276 + -1.2
1,267,276 + +0.1
1,268,787 + +0.3
1,268,787 + +1.6
1,269,230 + +0.2

Or see this region's nucleotide sequence