Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhG and ybiH overlap by 1 nucleotides ybiH and rhlE are separated by 228 nucleotides rhlE and ybiA are separated by 231 nucleotides ybiA and dinG are separated by 119 nucleotides
b0795: ybhG - hypothetical protein (NCBI), at 828,197 to 829,195
ybhG
b0796: ybiH - putative transcriptional regulator (VIMSS), at 829,195 to 829,866
ybiH
b0797: rhlE - RNA helicase (NCBI), at 830,095 to 831,459
rhlE
b0798: ybiA - hypothetical protein (NCBI), at 831,691 to 832,173
ybiA
b0799: dinG - ATP-dependent DNA helicase (NCBI), at 832,293 to 834,443
dinG
Position (kb) 830
831
832 Strain fitness (log2 ratio) -2
-1
0
1
2 at 829.295 kb on - strand, within ybiH at 829.360 kb on + strand, within ybiH at 829.360 kb on + strand, within ybiH at 829.535 kb on - strand, within ybiH at 829.598 kb on + strand, within ybiH at 829.679 kb on + strand, within ybiH at 829.841 kb on - strand at 829.841 kb on - strand at 829.845 kb on - strand at 829.858 kb on + strand at 829.996 kb on + strand at 830.051 kb on + strand at 830.059 kb on + strand at 830.059 kb on + strand at 830.123 kb on + strand at 830.123 kb on + strand at 830.123 kb on + strand at 830.146 kb on + strand at 830.156 kb on + strand at 830.159 kb on - strand at 830.176 kb on - strand at 830.182 kb on + strand at 830.182 kb on + strand at 830.192 kb on - strand at 830.249 kb on - strand, within rhlE at 830.277 kb on - strand, within rhlE at 830.277 kb on - strand, within rhlE at 830.333 kb on + strand, within rhlE at 830.333 kb on + strand, within rhlE at 830.335 kb on - strand, within rhlE at 830.335 kb on - strand, within rhlE at 830.341 kb on - strand, within rhlE at 830.390 kb on + strand, within rhlE at 830.531 kb on + strand, within rhlE at 830.836 kb on + strand, within rhlE at 830.850 kb on - strand, within rhlE at 830.860 kb on - strand, within rhlE at 830.860 kb on - strand, within rhlE at 831.483 kb on + strand at 831.535 kb on - strand at 831.542 kb on - strand at 831.542 kb on - strand at 831.679 kb on + strand at 831.679 kb on + strand at 831.733 kb on - strand at 831.733 kb on - strand at 831.752 kb on + strand, within ybiA at 831.752 kb on + strand, within ybiA at 831.942 kb on - strand, within ybiA at 832.127 kb on + strand at 832.127 kb on + strand at 832.158 kb on - strand at 832.250 kb on + strand at 832.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 829,295 - ybiH b0796 0.15 -1.5 829,360 + ybiH b0796 0.25 +1.4 829,360 + ybiH b0796 0.25 +0.4 829,535 - ybiH b0796 0.51 -0.4 829,598 + ybiH b0796 0.60 -1.4 829,679 + ybiH b0796 0.72 -0.6 829,841 - -0.6 829,841 - -0.6 829,845 - -1.4 829,858 + -1.1 829,996 + -0.4 830,051 + +0.6 830,059 + +0.8 830,059 + +2.4 830,123 + +0.4 830,123 + +0.7 830,123 + +0.7 830,146 + +1.8 830,156 + +0.1 830,159 - +1.4 830,176 - -0.4 830,182 + +1.9 830,182 + +0.8 830,192 - +1.3 830,249 - rhlE b0797 0.11 -0.4 830,277 - rhlE b0797 0.13 -0.2 830,277 - rhlE b0797 0.13 +2.2 830,333 + rhlE b0797 0.17 +0.5 830,333 + rhlE b0797 0.17 +0.2 830,335 - rhlE b0797 0.18 +1.5 830,335 - rhlE b0797 0.18 +0.8 830,341 - rhlE b0797 0.18 +1.0 830,390 + rhlE b0797 0.22 +0.6 830,531 + rhlE b0797 0.32 +0.7 830,836 + rhlE b0797 0.54 +1.2 830,850 - rhlE b0797 0.55 +1.2 830,860 - rhlE b0797 0.56 +2.2 830,860 - rhlE b0797 0.56 +0.2 831,483 + +0.5 831,535 - -1.2 831,542 - -0.5 831,542 - +1.2 831,679 + -0.8 831,679 + -0.1 831,733 - +1.2 831,733 - +0.2 831,752 + ybiA b0798 0.13 +0.4 831,752 + ybiA b0798 0.13 +1.8 831,942 - ybiA b0798 0.52 +0.7 832,127 + -1.8 832,127 + +0.2 832,158 - +0.4 832,250 + +1.2 832,353 - -0.6
Or see this region's nucleotide sequence