Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
Position (kb) 810
811
812 Strain fitness (log2 ratio) -2
-1
0
1
2 at 809.993 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.359 kb on + strand, within bioF at 810.362 kb on - strand, within bioF at 810.393 kb on - strand, within bioF at 810.414 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD at 812.000 kb on + strand, within bioD at 812.005 kb on - strand, within bioD at 812.005 kb on - strand, within bioD at 812.051 kb on + strand, within bioD at 812.051 kb on + strand, within bioD at 812.061 kb on - strand, within bioD at 812.295 kb on - strand at 812.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 809,993 + bioF b0776 0.34 -1.4 809,993 + bioF b0776 0.34 -1.1 810,237 + bioF b0776 0.55 +0.6 810,237 + bioF b0776 0.55 -1.8 810,359 + bioF b0776 0.65 -0.5 810,362 - bioF b0776 0.66 -0.6 810,393 - bioF b0776 0.68 -0.7 810,414 + bioF b0776 0.70 +0.5 810,492 + bioF b0776 0.77 -0.2 810,492 + bioF b0776 0.77 +1.2 810,673 + -0.4 810,673 - -0.4 810,729 + +0.9 810,791 + -0.5 810,833 + bioC b0777 0.12 +0.5 810,971 + bioC b0777 0.30 +0.7 811,122 + bioC b0777 0.50 -0.0 811,122 + bioC b0777 0.50 -1.1 811,122 + bioC b0777 0.50 +0.7 811,122 + bioC b0777 0.50 +0.9 811,326 + bioC b0777 0.77 +0.7 811,326 + bioC b0777 0.77 +1.2 811,326 + bioC b0777 0.77 +2.0 811,366 - bioC b0777 0.82 +2.2 811,423 + bioC b0777 0.90 +0.7 811,423 + bioC b0777 0.90 -0.2 811,431 - -1.9 811,446 - +0.2 811,446 - +0.6 811,464 - -0.0 811,559 + +1.1 811,672 + bioD b0778 0.26 -0.8 811,720 - bioD b0778 0.33 -0.1 811,757 + bioD b0778 0.39 +0.6 812,000 + bioD b0778 0.75 +1.5 812,005 - bioD b0778 0.76 +2.2 812,005 - bioD b0778 0.76 +0.5 812,051 + bioD b0778 0.82 +1.5 812,051 + bioD b0778 0.82 -0.2 812,061 - bioD b0778 0.84 -0.4 812,295 - -1.9 812,353 - +1.5
Or see this region's nucleotide sequence