Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides fldA and ybfE are separated by 139 nucleotides ybfE and ybfF are separated by 139 nucleotides
b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869
fur
b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948
uof
b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688
fldA
b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121
ybfE
b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025
ybfF
Position (kb) 710
711
712 Strain fitness (log2 ratio) -2
-1
0
1
2 at 709.861 kb on - strand at 710.067 kb on - strand at 710.080 kb on + strand at 710.148 kb on - strand at 710.148 kb on - strand at 710.163 kb on - strand at 710.751 kb on + strand at 710.780 kb on - strand at 710.780 kb on - strand at 710.787 kb on + strand at 710.798 kb on + strand at 710.805 kb on - strand at 710.805 kb on - strand at 710.807 kb on + strand at 710.901 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 711.159 kb on - strand at 711.400 kb on - strand, within ybfF at 711.569 kb on + strand, within ybfF at 711.668 kb on - strand, within ybfF at 711.685 kb on + strand, within ybfF at 711.728 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.900 kb on - strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.933 kb on + strand, within ybfF at 712.060 kb on + strand at 712.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 709,861 - +0.4 710,067 - -2.2 710,080 + -0.2 710,148 - -1.1 710,148 - +0.7 710,163 - -0.1 710,751 + -1.4 710,780 - +0.9 710,780 - -2.2 710,787 + +0.7 710,798 + +2.4 710,805 - +0.9 710,805 - -1.4 710,807 + +1.2 710,901 + ybfE b0685 0.25 +2.4 710,924 + ybfE b0685 0.33 +2.7 710,924 + ybfE b0685 0.33 +1.7 711,159 - +0.2 711,400 - ybfF b0686 0.18 -0.3 711,569 + ybfF b0686 0.40 -0.2 711,668 - ybfF b0686 0.53 +2.1 711,685 + ybfF b0686 0.55 +1.2 711,728 - ybfF b0686 0.61 -1.1 711,730 - ybfF b0686 0.61 -0.3 711,730 - ybfF b0686 0.61 +1.2 711,803 - ybfF b0686 0.71 +1.2 711,803 - ybfF b0686 0.71 +0.8 711,900 - ybfF b0686 0.84 +1.5 711,923 + ybfF b0686 0.87 +1.1 711,923 + ybfF b0686 0.87 +1.2 711,933 + ybfF b0686 0.88 +0.6 712,060 + -0.8 712,077 + +1.2
Or see this region's nucleotide sequence