Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides queA and tgt are separated by 55 nucleotides
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
b0406: tgt - queuine tRNA-ribosyltransferase (NCBI), at 425,361 to 426,488
tgt
Position (kb) 424
425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA at 424.612 kb on + strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.651 kb on - strand, within queA at 424.657 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.668 kb on + strand, within queA at 424.922 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.147 kb on - strand, within queA at 425.300 kb on + strand at 425.300 kb on + strand at 425.436 kb on - strand at 425.470 kb on + strand at 425.700 kb on - strand, within tgt at 425.700 kb on - strand, within tgt at 425.852 kb on - strand, within tgt at 425.914 kb on + strand, within tgt at 425.945 kb on - strand, within tgt at 425.945 kb on - strand at 425.964 kb on + strand, within tgt at 426.030 kb on - strand, within tgt at 426.030 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.035 kb on - strand, within tgt at 426.230 kb on + strand, within tgt at 426.230 kb on + strand, within tgt at 426.241 kb on + strand, within tgt at 426.249 kb on - strand, within tgt at 426.259 kb on - strand, within tgt at 426.277 kb on - strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 423,283 - malZ b0403 0.85 +1.2 423,401 - -1.9 423,404 - +0.9 423,404 - -0.0 423,445 - +0.2 423,512 + +2.6 423,548 - -1.4 423,548 - -0.6 423,711 + yajB b0404 0.26 +0.0 423,711 + yajB b0404 0.26 -1.9 423,909 + yajB b0404 0.60 -1.1 423,909 + +1.8 423,923 + yajB b0404 0.62 -1.8 423,996 + yajB b0404 0.75 +0.1 424,011 - yajB b0404 0.77 +1.2 424,112 + -1.5 424,121 - -0.2 424,121 - +0.2 424,232 + +0.5 424,232 + +0.6 424,280 + -0.4 424,280 + -1.9 424,484 - queA b0405 0.23 -2.6 424,484 - queA b0405 0.23 -1.3 424,612 + queA b0405 0.35 -0.6 424,651 - queA b0405 0.39 +0.1 424,651 - queA b0405 0.39 -2.7 424,651 - queA b0405 0.39 -0.9 424,657 + queA b0405 0.39 +0.2 424,668 + queA b0405 0.40 -0.8 424,668 + queA b0405 0.40 -0.2 424,922 - queA b0405 0.64 -0.6 425,147 - queA b0405 0.85 -0.6 425,147 - queA b0405 0.85 -0.5 425,300 + -1.3 425,300 + +0.2 425,436 - -1.2 425,470 + -0.1 425,700 - tgt b0406 0.30 -1.8 425,700 - tgt b0406 0.30 -1.1 425,852 - tgt b0406 0.44 -1.8 425,914 + tgt b0406 0.49 -1.2 425,945 - tgt b0406 0.52 -0.6 425,945 - +1.2 425,964 + tgt b0406 0.53 -1.7 426,030 - tgt b0406 0.59 +0.2 426,030 - tgt b0406 0.59 -1.1 426,035 - tgt b0406 0.60 -1.1 426,035 - tgt b0406 0.60 -1.8 426,230 + tgt b0406 0.77 -1.1 426,230 + tgt b0406 0.77 +0.2 426,241 + tgt b0406 0.78 +0.2 426,249 - tgt b0406 0.79 -1.9 426,259 - tgt b0406 0.80 -1.5 426,277 - tgt b0406 0.81 -0.2
Or see this region's nucleotide sequence