Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insD-1 and yaiP are separated by 159 nucleotides yaiP and yaiS are separated by 123 nucleotides
b0361: insD-1 - IS2 insertion element transposase InsAB' (NCBI), at 380,898 to 381,803
insD-1
b0363: yaiP - predicted glucosyltransferase (NCBI), at 381,963 to 383,159
yaiP
b0364: yaiS - orf, hypothetical protein (VIMSS), at 383,283 to 383,840
yaiS
Position (kb) 381
382
383
384 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 381.766 kb on + strand at 381.771 kb on + strand at 381.771 kb on + strand at 381.777 kb on + strand at 381.839 kb on + strand at 381.844 kb on + strand at 381.854 kb on + strand at 381.854 kb on + strand at 381.932 kb on + strand at 381.995 kb on - strand at 382.007 kb on - strand at 382.014 kb on - strand at 382.014 kb on - strand at 382.023 kb on - strand at 382.023 kb on - strand at 382.033 kb on + strand at 382.039 kb on - strand at 382.039 kb on - strand at 382.041 kb on - strand at 382.048 kb on + strand at 382.048 kb on + strand at 382.048 kb on + strand at 382.048 kb on + strand at 382.056 kb on - strand at 382.058 kb on + strand at 382.064 kb on - strand at 382.153 kb on - strand, within yaiP at 382.153 kb on - strand, within yaiP at 382.153 kb on - strand, within yaiP at 382.163 kb on + strand, within yaiP at 382.163 kb on + strand, within yaiP at 382.169 kb on - strand, within yaiP at 382.171 kb on + strand, within yaiP at 382.171 kb on + strand, within yaiP at 382.210 kb on + strand, within yaiP at 382.214 kb on - strand, within yaiP at 382.225 kb on + strand, within yaiP at 382.247 kb on - strand, within yaiP at 382.255 kb on - strand, within yaiP at 382.261 kb on + strand, within yaiP at 382.269 kb on - strand, within yaiP at 382.272 kb on - strand, within yaiP at 382.306 kb on + strand, within yaiP at 382.326 kb on - strand, within yaiP at 382.326 kb on - strand, within yaiP at 382.375 kb on - strand, within yaiP at 382.375 kb on - strand, within yaiP at 382.383 kb on + strand, within yaiP at 382.398 kb on + strand, within yaiP at 382.423 kb on - strand, within yaiP at 382.423 kb on - strand, within yaiP at 382.428 kb on - strand, within yaiP at 382.428 kb on - strand, within yaiP at 382.438 kb on - strand, within yaiP at 382.451 kb on + strand, within yaiP at 382.459 kb on - strand, within yaiP at 382.527 kb on + strand, within yaiP at 382.535 kb on - strand, within yaiP at 382.535 kb on - strand, within yaiP at 382.576 kb on + strand, within yaiP at 382.576 kb on + strand, within yaiP at 382.576 kb on + strand, within yaiP at 382.613 kb on + strand, within yaiP at 382.618 kb on + strand, within yaiP at 382.631 kb on + strand, within yaiP at 382.637 kb on - strand, within yaiP at 382.665 kb on + strand, within yaiP at 382.694 kb on - strand, within yaiP at 382.694 kb on - strand, within yaiP at 382.714 kb on + strand, within yaiP at 382.771 kb on - strand, within yaiP at 382.771 kb on - strand, within yaiP at 382.772 kb on - strand, within yaiP at 382.787 kb on - strand, within yaiP at 382.793 kb on + strand, within yaiP at 382.793 kb on + strand, within yaiP at 382.795 kb on + strand, within yaiP at 382.795 kb on + strand, within yaiP at 382.809 kb on - strand, within yaiP at 382.809 kb on - strand, within yaiP at 382.861 kb on + strand, within yaiP at 382.940 kb on + strand, within yaiP at 382.942 kb on + strand, within yaiP at 382.942 kb on + strand, within yaiP at 382.947 kb on + strand, within yaiP at 382.950 kb on + strand, within yaiP at 382.950 kb on + strand, within yaiP at 382.953 kb on - strand, within yaiP at 382.953 kb on - strand, within yaiP at 382.987 kb on + strand, within yaiP at 383.000 kb on + strand, within yaiP at 383.011 kb on + strand, within yaiP at 383.021 kb on + strand, within yaiP at 383.021 kb on + strand, within yaiP at 383.026 kb on + strand, within yaiP at 383.034 kb on - strand, within yaiP at 383.046 kb on - strand at 383.048 kb on - strand at 383.048 kb on - strand at 383.048 kb on - strand at 383.050 kb on + strand at 383.051 kb on + strand at 383.051 kb on + strand at 383.065 kb on + strand at 383.086 kb on + strand at 383.091 kb on + strand at 383.091 kb on + strand at 383.092 kb on + strand at 383.111 kb on + strand at 383.189 kb on + strand at 383.189 kb on + strand at 383.196 kb on + strand at 383.203 kb on + strand at 383.203 kb on + strand at 383.203 kb on + strand at 383.207 kb on + strand at 383.207 kb on + strand at 383.212 kb on + strand at 383.212 kb on + strand at 383.212 kb on + strand at 383.220 kb on - strand at 383.225 kb on - strand at 383.235 kb on + strand at 383.377 kb on + strand, within yaiS at 383.377 kb on + strand, within yaiS at 383.381 kb on + strand, within yaiS at 383.391 kb on + strand, within yaiS at 383.392 kb on - strand, within yaiS at 383.392 kb on - strand, within yaiS at 383.409 kb on + strand, within yaiS at 383.452 kb on + strand, within yaiS at 383.467 kb on + strand, within yaiS at 383.472 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.479 kb on + strand, within yaiS at 383.481 kb on - strand, within yaiS at 383.487 kb on - strand, within yaiS at 383.551 kb on + strand, within yaiS at 383.561 kb on + strand, within yaiS at 383.617 kb on - strand, within yaiS
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 381,766 + -3.4 381,771 + +1.8 381,771 + +2.3 381,777 + +0.1 381,839 + -2.1 381,844 + -1.5 381,854 + +0.1 381,854 + -0.9 381,932 + -0.3 381,995 - -0.7 382,007 - +0.1 382,014 - -0.5 382,014 - -0.4 382,023 - -0.1 382,023 - -0.1 382,033 + -0.4 382,039 - -0.2 382,039 - +2.1 382,041 - +0.8 382,048 + -0.6 382,048 + +1.5 382,048 + +0.5 382,048 + -0.8 382,056 - +0.4 382,058 + +0.3 382,064 - +2.7 382,153 - yaiP b0363 0.16 +0.0 382,153 - yaiP b0363 0.16 -0.4 382,153 - yaiP b0363 0.16 +1.9 382,163 + yaiP b0363 0.17 -0.7 382,163 + yaiP b0363 0.17 +0.9 382,169 - yaiP b0363 0.17 -0.4 382,171 + yaiP b0363 0.17 +0.7 382,171 + yaiP b0363 0.17 -1.3 382,210 + yaiP b0363 0.21 -0.6 382,214 - yaiP b0363 0.21 +0.3 382,225 + yaiP b0363 0.22 -1.1 382,247 - yaiP b0363 0.24 +0.4 382,255 - yaiP b0363 0.24 -0.1 382,261 + yaiP b0363 0.25 -0.9 382,269 - yaiP b0363 0.26 -0.0 382,272 - yaiP b0363 0.26 +0.3 382,306 + yaiP b0363 0.29 +0.1 382,326 - yaiP b0363 0.30 -0.0 382,326 - yaiP b0363 0.30 -0.2 382,375 - yaiP b0363 0.34 +0.5 382,375 - yaiP b0363 0.34 -1.5 382,383 + yaiP b0363 0.35 -0.8 382,398 + yaiP b0363 0.36 -0.6 382,423 - yaiP b0363 0.38 -0.5 382,423 - yaiP b0363 0.38 +0.4 382,428 - yaiP b0363 0.39 +1.5 382,428 - yaiP b0363 0.39 -0.3 382,438 - yaiP b0363 0.40 -1.1 382,451 + yaiP b0363 0.41 +0.9 382,459 - yaiP b0363 0.41 +0.3 382,527 + yaiP b0363 0.47 -1.0 382,535 - yaiP b0363 0.48 -0.9 382,535 - yaiP b0363 0.48 -0.6 382,576 + yaiP b0363 0.51 -1.3 382,576 + yaiP b0363 0.51 +0.3 382,576 + yaiP b0363 0.51 +0.8 382,613 + yaiP b0363 0.54 -0.3 382,618 + yaiP b0363 0.55 -0.2 382,631 + yaiP b0363 0.56 -0.1 382,637 - yaiP b0363 0.56 +0.6 382,665 + yaiP b0363 0.59 -0.2 382,694 - yaiP b0363 0.61 -0.7 382,694 - yaiP b0363 0.61 +1.0 382,714 + yaiP b0363 0.63 +2.6 382,771 - yaiP b0363 0.68 -0.3 382,771 - yaiP b0363 0.68 +0.7 382,772 - yaiP b0363 0.68 +0.7 382,787 - yaiP b0363 0.69 +0.6 382,793 + yaiP b0363 0.69 +1.3 382,793 + yaiP b0363 0.69 +0.0 382,795 + yaiP b0363 0.70 +2.1 382,795 + yaiP b0363 0.70 -1.3 382,809 - yaiP b0363 0.71 -1.7 382,809 - yaiP b0363 0.71 -0.7 382,861 + yaiP b0363 0.75 -1.4 382,940 + yaiP b0363 0.82 -0.7 382,942 + yaiP b0363 0.82 +1.7 382,942 + yaiP b0363 0.82 +2.4 382,947 + yaiP b0363 0.82 +0.6 382,950 + yaiP b0363 0.82 +1.5 382,950 + yaiP b0363 0.82 -0.4 382,953 - yaiP b0363 0.83 -0.1 382,953 - yaiP b0363 0.83 -0.4 382,987 + yaiP b0363 0.86 -0.3 383,000 + yaiP b0363 0.87 +0.5 383,011 + yaiP b0363 0.88 +0.9 383,021 + yaiP b0363 0.88 +0.5 383,021 + yaiP b0363 0.88 +1.3 383,026 + yaiP b0363 0.89 +0.5 383,034 - yaiP b0363 0.89 -0.7 383,046 - +0.5 383,048 - +0.0 383,048 - -0.8 383,048 - +0.3 383,050 + -2.8 383,051 + +0.7 383,051 + -0.0 383,065 + +1.9 383,086 + +0.7 383,091 + -1.5 383,091 + +0.9 383,092 + +0.0 383,111 + -0.3 383,189 + +1.3 383,189 + +1.3 383,196 + -0.3 383,203 + -0.1 383,203 + -1.1 383,203 + +0.1 383,207 + +2.5 383,207 + -0.7 383,212 + +0.8 383,212 + +1.3 383,212 + +2.3 383,220 - -0.8 383,225 - +1.1 383,235 + -2.4 383,377 + yaiS b0364 0.17 +0.4 383,377 + yaiS b0364 0.17 +0.9 383,381 + yaiS b0364 0.18 +0.6 383,391 + yaiS b0364 0.19 +1.1 383,392 - yaiS b0364 0.20 -2.3 383,392 - yaiS b0364 0.20 +0.9 383,409 + yaiS b0364 0.23 -2.1 383,452 + yaiS b0364 0.30 -0.7 383,467 + yaiS b0364 0.33 -1.3 383,472 + yaiS b0364 0.34 +0.8 383,479 + yaiS b0364 0.35 -0.1 383,479 + yaiS b0364 0.35 -0.3 383,479 + yaiS b0364 0.35 -0.3 383,479 + yaiS b0364 0.35 +0.9 383,479 + yaiS b0364 0.35 -1.3 383,479 + yaiS b0364 0.35 +0.7 383,479 + yaiS b0364 0.35 -0.6 383,479 + yaiS b0364 0.35 +0.3 383,479 + yaiS b0364 0.35 +1.3 383,479 + yaiS b0364 0.35 +0.1 383,479 + yaiS b0364 0.35 -0.2 383,481 - yaiS b0364 0.35 -1.3 383,487 - yaiS b0364 0.37 -0.7 383,551 + yaiS b0364 0.48 +1.8 383,561 + yaiS b0364 0.50 +0.3 383,617 - yaiS b0364 0.60 -2.6
Or see this region's nucleotide sequence