Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpD and mhpF overlap by 4 nucleotides mhpF and mhpE overlap by 4 nucleotides mhpE and mhpT are separated by 577 nucleotides
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
b0351: mhpF - acetaldehyde dehydrogenase (NCBI), at 372,145 to 373,095
mhpF
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894
mhpT
Position (kb) 373
374
375 Strain fitness (log2 ratio) -2
-1
0
1 at 372.181 kb on - strand at 372.181 kb on - strand at 372.191 kb on - strand at 372.191 kb on - strand at 372.193 kb on + strand at 372.193 kb on + strand at 372.226 kb on + strand at 372.226 kb on + strand at 372.271 kb on + strand, within mhpF at 372.307 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.619 kb on - strand, within mhpF at 372.648 kb on + strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.793 kb on + strand, within mhpF at 372.814 kb on + strand, within mhpF at 372.820 kb on + strand, within mhpF at 372.844 kb on - strand, within mhpF at 372.854 kb on - strand, within mhpF at 372.903 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 373.062 kb on + strand at 373.130 kb on + strand at 373.130 kb on + strand at 373.393 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.437 kb on - strand, within mhpE at 373.552 kb on - strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.736 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.912 kb on - strand, within mhpE at 374.415 kb on + strand at 374.631 kb on - strand at 374.643 kb on - strand at 374.643 kb on - strand at 374.686 kb on - strand at 374.686 kb on - strand at 374.715 kb on - strand at 374.796 kb on + strand at 374.796 kb on + strand at 374.877 kb on - strand, within mhpT at 374.877 kb on - strand, within mhpT at 374.914 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.030 kb on + strand, within mhpT at 375.044 kb on + strand, within mhpT at 375.048 kb on - strand, within mhpT
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 372,181 - -0.1 372,181 - +1.6 372,191 - +0.3 372,191 - +0.3 372,193 + -0.7 372,193 + +0.8 372,226 + +1.3 372,226 + +0.5 372,271 + mhpF b0351 0.13 +0.3 372,307 + mhpF b0351 0.17 +0.4 372,308 + mhpF b0351 0.17 -0.6 372,308 + mhpF b0351 0.17 +0.3 372,308 + mhpF b0351 0.17 -0.7 372,619 - mhpF b0351 0.50 +0.9 372,648 + mhpF b0351 0.53 -0.3 372,779 - mhpF b0351 0.67 +0.3 372,779 - mhpF b0351 0.67 -0.3 372,793 + mhpF b0351 0.68 -0.5 372,814 + mhpF b0351 0.70 -1.6 372,820 + mhpF b0351 0.71 +1.1 372,844 - mhpF b0351 0.74 -0.3 372,854 - mhpF b0351 0.75 +1.0 372,903 + mhpF b0351 0.80 -0.9 372,991 + mhpF b0351 0.89 +0.3 372,991 + mhpF b0351 0.89 -0.3 373,062 + -0.0 373,130 + -2.1 373,130 + -0.2 373,393 + mhpE b0352 0.30 +0.9 373,429 + mhpE b0352 0.33 +1.0 373,429 + mhpE b0352 0.33 -1.0 373,437 - mhpE b0352 0.34 +0.3 373,552 - mhpE b0352 0.45 +0.5 373,576 + mhpE b0352 0.48 -1.1 373,576 + mhpE b0352 0.48 -0.7 373,640 + mhpE b0352 0.54 -1.3 373,640 + mhpE b0352 0.54 +0.7 373,736 - mhpE b0352 0.64 -0.2 373,787 - mhpE b0352 0.69 +1.2 373,787 - mhpE b0352 0.69 +1.0 373,857 - mhpE b0352 0.75 -0.3 373,857 - mhpE b0352 0.75 +0.3 373,912 - mhpE b0352 0.81 -1.6 374,415 + -0.7 374,631 - +0.6 374,643 - +0.4 374,643 - -1.3 374,686 - -1.7 374,686 - +0.3 374,715 - -1.0 374,796 + -1.1 374,796 + -0.1 374,877 - mhpT b0353 0.16 -0.1 374,877 - mhpT b0353 0.16 +0.5 374,914 + mhpT b0353 0.19 +1.3 375,030 + mhpT b0353 0.29 +0.9 375,030 + mhpT b0353 0.29 +0.9 375,044 + mhpT b0353 0.30 +0.3 375,048 - mhpT b0353 0.30 -1.4
Or see this region's nucleotide sequence