Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaA and nhaR are separated by 59 nucleotides nhaR and insB-1 are separated by 190 nucleotides insB-1 and insA-1 overlap by 82 nucleotides
b0019: nhaA - pH-dependent sodium/proton antiporter (NCBI), at 17,489 to 18,655
nhaA
b0020: nhaR - DNA-binding transcriptional activator (NCBI), at 18,715 to 19,620
nhaR
b0021: insB-1 - IS1 transposase InsAB' (NCBI), at 19,811 to 20,314
insB-1
b0022: insA-1 - IS1 repressor protein InsA (NCBI), at 20,233 to 20,508
insA-1
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.749 kb on - strand, within nhaA at 17.749 kb on - strand, within nhaA at 17.765 kb on - strand, within nhaA at 17.765 kb on - strand, within nhaA at 17.813 kb on + strand, within nhaA at 17.879 kb on + strand, within nhaA at 17.887 kb on - strand, within nhaA at 17.902 kb on - strand, within nhaA at 17.904 kb on + strand, within nhaA at 17.917 kb on + strand, within nhaA at 17.925 kb on - strand, within nhaA at 17.925 kb on - strand, within nhaA at 17.925 kb on - strand, within nhaA at 17.990 kb on + strand, within nhaA at 17.990 kb on + strand, within nhaA at 18.015 kb on - strand, within nhaA at 18.041 kb on - strand, within nhaA at 18.044 kb on - strand, within nhaA at 18.044 kb on - strand, within nhaA at 18.044 kb on - strand, within nhaA at 18.085 kb on + strand, within nhaA at 18.085 kb on + strand, within nhaA at 18.095 kb on - strand, within nhaA at 18.097 kb on - strand at 18.097 kb on - strand, within nhaA at 18.098 kb on - strand, within nhaA at 18.098 kb on - strand, within nhaA at 18.128 kb on + strand, within nhaA at 18.167 kb on + strand, within nhaA at 18.201 kb on - strand, within nhaA at 18.244 kb on - strand, within nhaA at 18.245 kb on + strand, within nhaA at 18.329 kb on - strand, within nhaA at 18.353 kb on - strand, within nhaA at 18.353 kb on - strand, within nhaA at 18.480 kb on + strand, within nhaA at 18.488 kb on - strand, within nhaA at 18.501 kb on + strand, within nhaA at 18.501 kb on + strand, within nhaA at 18.557 kb on - strand at 18.557 kb on - strand at 18.559 kb on + strand at 18.567 kb on - strand at 18.567 kb on - strand at 18.576 kb on + strand at 18.591 kb on + strand at 18.591 kb on + strand at 18.596 kb on + strand at 18.619 kb on - strand at 18.619 kb on - strand at 18.619 kb on - strand at 18.753 kb on - strand at 18.765 kb on + strand at 18.809 kb on - strand, within nhaR at 18.809 kb on - strand, within nhaR at 18.842 kb on - strand, within nhaR at 18.852 kb on - strand, within nhaR at 18.852 kb on - strand, within nhaR at 18.852 kb on - strand, within nhaR at 18.898 kb on - strand, within nhaR at 18.917 kb on - strand, within nhaR at 18.917 kb on - strand, within nhaR at 19.027 kb on + strand, within nhaR at 19.036 kb on + strand, within nhaR at 19.066 kb on + strand, within nhaR at 19.068 kb on + strand, within nhaR at 19.068 kb on + strand, within nhaR at 19.074 kb on - strand, within nhaR at 19.105 kb on - strand, within nhaR at 19.148 kb on - strand, within nhaR at 19.194 kb on - strand, within nhaR at 19.241 kb on + strand, within nhaR at 19.244 kb on - strand, within nhaR at 19.244 kb on - strand, within nhaR at 19.275 kb on - strand, within nhaR at 19.319 kb on - strand, within nhaR at 19.326 kb on - strand, within nhaR at 19.326 kb on - strand, within nhaR at 19.425 kb on - strand, within nhaR at 19.457 kb on - strand, within nhaR at 19.464 kb on + strand, within nhaR at 19.483 kb on - strand, within nhaR at 19.510 kb on + strand, within nhaR at 19.516 kb on + strand, within nhaR at 19.516 kb on + strand, within nhaR at 19.524 kb on - strand, within nhaR at 19.549 kb on + strand at 19.549 kb on + strand at 19.549 kb on + strand at 19.619 kb on + strand at 19.644 kb on + strand at 19.669 kb on - strand at 19.723 kb on + strand at 19.723 kb on + strand at 19.723 kb on - strand at 20.506 kb on + strand at 20.574 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 17,749 - nhaA b0019 0.22 -1.0 17,749 - nhaA b0019 0.22 -2.1 17,765 - nhaA b0019 0.24 -1.5 17,765 - nhaA b0019 0.24 +1.9 17,813 + nhaA b0019 0.28 +1.1 17,879 + nhaA b0019 0.33 -1.0 17,887 - nhaA b0019 0.34 +0.5 17,902 - nhaA b0019 0.35 -2.0 17,904 + nhaA b0019 0.36 +1.9 17,917 + nhaA b0019 0.37 -0.6 17,925 - nhaA b0019 0.37 -2.0 17,925 - nhaA b0019 0.37 -0.6 17,925 - nhaA b0019 0.37 +2.4 17,990 + nhaA b0019 0.43 +0.4 17,990 + nhaA b0019 0.43 -0.6 18,015 - nhaA b0019 0.45 -2.2 18,041 - nhaA b0019 0.47 -1.0 18,044 - nhaA b0019 0.48 +0.8 18,044 - nhaA b0019 0.48 -0.2 18,044 - nhaA b0019 0.48 -2.2 18,085 + nhaA b0019 0.51 -1.0 18,085 + nhaA b0019 0.51 -0.1 18,095 - nhaA b0019 0.52 +1.9 18,097 - +1.4 18,097 - nhaA b0019 0.52 +1.9 18,098 - nhaA b0019 0.52 -2.1 18,098 - nhaA b0019 0.52 +1.6 18,128 + nhaA b0019 0.55 +0.0 18,167 + nhaA b0019 0.58 -0.6 18,201 - nhaA b0019 0.61 +0.4 18,244 - nhaA b0019 0.65 +0.2 18,245 + nhaA b0019 0.65 -1.1 18,329 - nhaA b0019 0.72 -1.5 18,353 - nhaA b0019 0.74 -1.2 18,353 - nhaA b0019 0.74 -1.8 18,480 + nhaA b0019 0.85 -0.1 18,488 - nhaA b0019 0.86 -1.1 18,501 + nhaA b0019 0.87 -1.6 18,501 + nhaA b0019 0.87 +0.4 18,557 - +1.4 18,557 - +1.9 18,559 + -2.2 18,567 - -0.2 18,567 - +0.4 18,576 + +1.4 18,591 + +0.2 18,591 + +2.5 18,596 + -0.6 18,619 - -0.6 18,619 - +0.0 18,619 - +1.1 18,753 - -0.2 18,765 + +0.2 18,809 - nhaR b0020 0.10 -1.8 18,809 - nhaR b0020 0.10 -0.6 18,842 - nhaR b0020 0.14 -0.4 18,852 - nhaR b0020 0.15 -1.6 18,852 - nhaR b0020 0.15 -1.5 18,852 - nhaR b0020 0.15 +1.8 18,898 - nhaR b0020 0.20 -0.3 18,917 - nhaR b0020 0.22 -0.3 18,917 - nhaR b0020 0.22 -0.7 19,027 + nhaR b0020 0.34 -0.6 19,036 + nhaR b0020 0.35 -0.9 19,066 + nhaR b0020 0.39 -0.1 19,068 + nhaR b0020 0.39 +0.2 19,068 + nhaR b0020 0.39 +0.2 19,074 - nhaR b0020 0.40 -0.8 19,105 - nhaR b0020 0.43 +0.9 19,148 - nhaR b0020 0.48 -0.6 19,194 - nhaR b0020 0.53 -1.2 19,241 + nhaR b0020 0.58 -0.6 19,244 - nhaR b0020 0.58 +1.8 19,244 - nhaR b0020 0.58 -0.8 19,275 - nhaR b0020 0.62 -0.5 19,319 - nhaR b0020 0.67 -1.1 19,326 - nhaR b0020 0.67 +0.0 19,326 - nhaR b0020 0.67 -0.6 19,425 - nhaR b0020 0.78 -2.2 19,457 - nhaR b0020 0.82 +0.9 19,464 + nhaR b0020 0.83 +1.1 19,483 - nhaR b0020 0.85 -0.5 19,510 + nhaR b0020 0.88 -2.6 19,516 + nhaR b0020 0.88 -0.6 19,516 + nhaR b0020 0.88 +0.4 19,524 - nhaR b0020 0.89 -0.9 19,549 + -1.5 19,549 + +0.7 19,549 + -1.5 19,619 + +1.4 19,644 + +0.6 19,669 - +1.7 19,723 + -0.4 19,723 + +1.1 19,723 - -0.6 20,506 + -0.8 20,574 - -1.6
Or see this region's nucleotide sequence