Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides thrC and yaaX are separated by 213 nucleotides yaaX and yaaA are separated by 152 nucleotides
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
b0005: yaaX - hypothetical protein (NCBI), at 5,234 to 5,530
yaaX
b0006: yaaA - hypothetical protein (NCBI), at 5,683 to 6,459
yaaA
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand at 3.805 kb on - strand at 3.953 kb on - strand, within thrC at 3.953 kb on - strand, within thrC at 4.108 kb on - strand, within thrC at 4.174 kb on - strand, within thrC at 4.180 kb on + strand, within thrC at 4.180 kb on + strand, within thrC at 4.216 kb on + strand, within thrC at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.755 kb on - strand, within thrC at 4.771 kb on + strand, within thrC at 4.771 kb on + strand, within thrC at 4.779 kb on - strand, within thrC at 4.779 kb on - strand, within thrC at 4.796 kb on + strand, within thrC at 4.875 kb on + strand, within thrC at 4.932 kb on + strand at 4.932 kb on + strand at 4.993 kb on + strand at 5.238 kb on + strand at 5.241 kb on + strand at 5.241 kb on + strand at 5.273 kb on + strand, within yaaX at 5.273 kb on + strand, within yaaX at 5.280 kb on - strand, within yaaX at 5.283 kb on + strand, within yaaX at 5.285 kb on + strand, within yaaX at 5.358 kb on - strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.470 kb on + strand, within yaaX at 5.639 kb on - strand at 5.699 kb on - strand at 5.699 kb on - strand at 5.788 kb on - strand, within yaaA at 5.788 kb on - strand, within yaaA at 5.835 kb on - strand, within yaaA at 5.879 kb on - strand, within yaaA at 5.891 kb on - strand, within yaaA at 5.898 kb on + strand, within yaaA at 5.958 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.976 kb on + strand, within yaaA at 5.995 kb on + strand, within yaaA at 6.005 kb on - strand, within yaaA
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 2,744 - -1.6 2,787 - -0.7 2,787 - +0.6 2,829 + +0.7 2,847 + -0.1 2,847 + +0.2 2,906 - thrB b0003 0.11 +0.4 2,934 + thrB b0003 0.14 -0.1 2,963 - thrB b0003 0.17 +0.4 3,020 - thrB b0003 0.23 -2.2 3,023 + thrB b0003 0.24 +1.4 3,131 + thrB b0003 0.35 -0.3 3,144 - thrB b0003 0.37 +0.6 3,186 + thrB b0003 0.41 +0.4 3,246 + thrB b0003 0.48 -1.6 3,248 + thrB b0003 0.48 -1.1 3,256 + thrB b0003 0.49 -2.2 3,293 + thrB b0003 0.53 +1.4 3,301 - thrB b0003 0.54 -1.5 3,318 - thrB b0003 0.55 -0.7 3,411 + thrB b0003 0.65 -0.1 3,444 - thrB b0003 0.69 -0.2 3,444 - thrB b0003 0.69 +2.9 3,504 - thrB b0003 0.75 +0.4 3,504 - thrB b0003 0.75 -0.1 3,509 - thrB b0003 0.76 -0.3 3,511 + thrB b0003 0.76 +1.4 3,607 + thrB b0003 0.86 -1.3 3,634 - thrB b0003 0.89 +0.1 3,638 + thrB b0003 0.90 -0.2 3,638 + thrB b0003 0.90 +0.8 3,650 + +0.5 3,769 + +1.6 3,769 + -1.8 3,792 - -0.6 3,805 - +0.0 3,953 - thrC b0004 0.17 +1.6 3,953 - thrC b0004 0.17 -2.7 4,108 - thrC b0004 0.29 +0.2 4,174 - thrC b0004 0.34 +0.8 4,180 + thrC b0004 0.35 +1.5 4,180 + thrC b0004 0.35 -1.3 4,216 + thrC b0004 0.37 +0.1 4,253 - thrC b0004 0.40 +1.4 4,253 - thrC b0004 0.40 -0.6 4,464 - thrC b0004 0.57 -1.5 4,466 + thrC b0004 0.57 -0.2 4,619 + thrC b0004 0.69 +0.8 4,721 + thrC b0004 0.77 -0.6 4,729 + thrC b0004 0.77 -0.6 4,729 - thrC b0004 0.77 -0.5 4,729 - thrC b0004 0.77 +0.4 4,755 - thrC b0004 0.79 +0.0 4,755 - thrC b0004 0.79 +0.4 4,755 - thrC b0004 0.79 +0.6 4,771 + thrC b0004 0.81 +0.0 4,771 + thrC b0004 0.81 -1.1 4,779 - thrC b0004 0.81 +0.2 4,779 - thrC b0004 0.81 -0.4 4,796 + thrC b0004 0.83 -0.5 4,875 + thrC b0004 0.89 +1.4 4,932 + +1.1 4,932 + -1.8 4,993 + +0.1 5,238 + -0.8 5,241 + -1.6 5,241 + +0.0 5,273 + yaaX b0005 0.13 -0.4 5,273 + yaaX b0005 0.13 +0.4 5,280 - yaaX b0005 0.15 +1.4 5,283 + yaaX b0005 0.16 -2.6 5,285 + yaaX b0005 0.17 -1.2 5,358 - yaaX b0005 0.42 -0.1 5,470 + yaaX b0005 0.79 +0.5 5,470 + yaaX b0005 0.79 -0.9 5,639 - -1.6 5,699 - -2.0 5,699 - +1.0 5,788 - yaaA b0006 0.14 -1.2 5,788 - yaaA b0006 0.14 +1.2 5,835 - yaaA b0006 0.20 -0.1 5,879 - yaaA b0006 0.25 -2.5 5,891 - yaaA b0006 0.27 -0.2 5,898 + yaaA b0006 0.28 -0.6 5,958 + yaaA b0006 0.35 -0.0 5,976 + yaaA b0006 0.38 -1.2 5,976 + yaaA b0006 0.38 -1.2 5,995 + yaaA b0006 0.40 -1.5 6,005 - yaaA b0006 0.41 -0.5
Or see this region's nucleotide sequence