Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16130 and AMB_RS16135 are separated by 88 nucleotides AMB_RS16135 and AMB_RS23515 are separated by 163 nucleotides AMB_RS23515 and AMB_RS16145 overlap by 11 nucleotides
AMB_RS16130: AMB_RS16130 - phosphoglycerate dehydrogenase, at 3,459,694 to 3,461,274
_RS16130
AMB_RS16135: AMB_RS16135 - phosphoserine transaminase, at 3,461,363 to 3,462,541
_RS16135
AMB_RS23515: AMB_RS23515 - zinc/iron-chelating domain-containing protein, at 3,462,705 to 3,463,490
_RS23515
AMB_RS16145: AMB_RS16145 - hybrid sensor histidine kinase/response regulator, at 3,463,480 to 3,465,285
_RS16145
Position (kb) 3461
3462
3463 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3460.769 kb on + strand, within AMB_RS16130 at 3461.148 kb on - strand at 3462.549 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.607 kb on - strand at 3462.640 kb on + strand at 3462.692 kb on + strand at 3462.692 kb on + strand at 3462.692 kb on + strand at 3462.692 kb on + strand at 3462.693 kb on - strand at 3462.693 kb on - strand at 3462.693 kb on - strand at 3462.693 kb on - strand at 3462.818 kb on - strand, within AMB_RS23515 at 3463.011 kb on + strand, within AMB_RS23515 at 3463.011 kb on + strand, within AMB_RS23515 at 3463.011 kb on + strand, within AMB_RS23515 at 3463.012 kb on - strand, within AMB_RS23515 at 3463.105 kb on + strand, within AMB_RS23515 at 3463.125 kb on + strand, within AMB_RS23515 at 3463.125 kb on + strand, within AMB_RS23515 at 3463.126 kb on - strand, within AMB_RS23515 at 3463.126 kb on - strand, within AMB_RS23515 at 3463.126 kb on - strand, within AMB_RS23515 at 3463.158 kb on + strand, within AMB_RS23515 at 3463.158 kb on + strand, within AMB_RS23515 at 3463.158 kb on + strand, within AMB_RS23515 at 3463.159 kb on - strand, within AMB_RS23515 at 3463.159 kb on - strand, within AMB_RS23515 at 3463.159 kb on - strand, within AMB_RS23515 at 3463.163 kb on + strand, within AMB_RS23515 at 3463.163 kb on + strand, within AMB_RS23515 at 3463.163 kb on + strand, within AMB_RS23515 at 3463.163 kb on + strand, within AMB_RS23515 at 3463.164 kb on - strand, within AMB_RS23515 at 3463.164 kb on - strand, within AMB_RS23515 at 3463.164 kb on - strand, within AMB_RS23515 at 3463.191 kb on + strand, within AMB_RS23515 at 3463.191 kb on + strand, within AMB_RS23515 at 3463.192 kb on - strand, within AMB_RS23515 at 3463.192 kb on - strand, within AMB_RS23515 at 3463.192 kb on - strand, within AMB_RS23515 at 3463.192 kb on - strand, within AMB_RS23515
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 3,460,769 + AMB_RS16130 0.68 -0.5 3,461,148 - -2.4 3,462,549 - +0.4 3,462,607 - -0.4 3,462,607 - +0.2 3,462,607 - -1.4 3,462,607 - -1.0 3,462,607 - -0.5 3,462,607 - -1.8 3,462,607 - -0.7 3,462,607 - -0.3 3,462,640 + +0.4 3,462,692 + -0.5 3,462,692 + +0.1 3,462,692 + -3.0 3,462,692 + +1.0 3,462,693 - -0.1 3,462,693 - -0.2 3,462,693 - -0.3 3,462,693 - +0.1 3,462,818 - AMB_RS23515 0.14 -3.0 3,463,011 + AMB_RS23515 0.39 -1.0 3,463,011 + AMB_RS23515 0.39 -1.3 3,463,011 + AMB_RS23515 0.39 -0.5 3,463,012 - AMB_RS23515 0.39 +0.2 3,463,105 + AMB_RS23515 0.51 -0.1 3,463,125 + AMB_RS23515 0.53 -2.5 3,463,125 + AMB_RS23515 0.53 -1.5 3,463,126 - AMB_RS23515 0.54 -2.8 3,463,126 - AMB_RS23515 0.54 -0.1 3,463,126 - AMB_RS23515 0.54 -1.5 3,463,158 + AMB_RS23515 0.58 -0.5 3,463,158 + AMB_RS23515 0.58 +0.2 3,463,158 + AMB_RS23515 0.58 -1.0 3,463,159 - AMB_RS23515 0.58 -1.0 3,463,159 - AMB_RS23515 0.58 -1.3 3,463,159 - AMB_RS23515 0.58 -2.7 3,463,163 + AMB_RS23515 0.58 -2.4 3,463,163 + AMB_RS23515 0.58 -1.5 3,463,163 + AMB_RS23515 0.58 +1.8 3,463,163 + AMB_RS23515 0.58 -0.5 3,463,164 - AMB_RS23515 0.58 -0.5 3,463,164 - AMB_RS23515 0.58 +0.5 3,463,164 - AMB_RS23515 0.58 -0.2 3,463,191 + AMB_RS23515 0.62 -2.5 3,463,191 + AMB_RS23515 0.62 +0.2 3,463,192 - AMB_RS23515 0.62 -0.1 3,463,192 - AMB_RS23515 0.62 +0.6 3,463,192 - AMB_RS23515 0.62 -0.5 3,463,192 - AMB_RS23515 0.62 -0.3
Or see this region's nucleotide sequence