Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14175 and AMB_RS14180 are separated by 161 nucleotides AMB_RS14180 and AMB_RS14185 are separated by 190 nucleotides AMB_RS14185 and AMB_RS14190 are separated by 196 nucleotides
AMB_RS14175: AMB_RS14175 - COQ9 family protein, at 3,009,307 to 3,009,948
_RS14175
AMB_RS14180: AMB_RS14180 - 30S ribosomal protein S21, at 3,010,110 to 3,010,313
_RS14180
AMB_RS14185: AMB_RS14185 - HAMP domain-containing protein, at 3,010,504 to 3,012,549
_RS14185
AMB_RS14190: AMB_RS14190 - cytochrome C biogenesis protein CcsA, at 3,012,746 to 3,013,729
_RS14190
Position (kb)
3010
3011
3012
3013 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3009.524 kb on + strand, within AMB_RS14175 at 3009.992 kb on + strand at 3009.992 kb on + strand at 3009.992 kb on + strand at 3009.992 kb on + strand at 3009.992 kb on + strand at 3010.312 kb on - strand at 3010.362 kb on + strand at 3010.364 kb on + strand at 3010.454 kb on - strand at 3010.511 kb on + strand at 3010.511 kb on + strand at 3010.592 kb on - strand at 3010.601 kb on + strand at 3010.602 kb on - strand at 3010.602 kb on - strand at 3010.717 kb on + strand, within AMB_RS14185 at 3010.717 kb on + strand, within AMB_RS14185 at 3010.718 kb on - strand, within AMB_RS14185 at 3010.718 kb on - strand, within AMB_RS14185 at 3010.945 kb on + strand, within AMB_RS14185 at 3010.946 kb on - strand, within AMB_RS14185 at 3010.946 kb on - strand, within AMB_RS14185 at 3011.036 kb on - strand, within AMB_RS14185 at 3011.125 kb on + strand, within AMB_RS14185 at 3011.126 kb on - strand, within AMB_RS14185 at 3011.201 kb on + strand, within AMB_RS14185 at 3011.201 kb on + strand, within AMB_RS14185 at 3012.547 kb on + strand at 3012.548 kb on - strand at 3012.626 kb on + strand at 3012.626 kb on + strand at 3012.632 kb on + strand at 3012.633 kb on - strand at 3012.747 kb on + strand at 3012.748 kb on - strand at 3012.748 kb on - strand at 3012.748 kb on - strand at 3012.951 kb on + strand, within AMB_RS14190 at 3012.951 kb on + strand, within AMB_RS14190 at 3012.952 kb on - strand, within AMB_RS14190 at 3012.957 kb on + strand, within AMB_RS14190 at 3012.957 kb on + strand, within AMB_RS14190 at 3012.957 kb on + strand, within AMB_RS14190 at 3012.958 kb on - strand, within AMB_RS14190 at 3012.958 kb on - strand, within AMB_RS14190 at 3012.958 kb on - strand, within AMB_RS14190 at 3013.059 kb on + strand, within AMB_RS14190 at 3013.060 kb on - strand, within AMB_RS14190 at 3013.060 kb on - strand, within AMB_RS14190 at 3013.152 kb on + strand, within AMB_RS14190
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 3,009,524 + AMB_RS14175 0.34 -2.0 3,009,992 + -0.7 3,009,992 + +1.4 3,009,992 + -3.7 3,009,992 + +1.4 3,009,992 + -1.8 3,010,312 - -2.0 3,010,362 + +0.4 3,010,364 + -2.0 3,010,454 - -3.3 3,010,511 + -0.3 3,010,511 + -1.0 3,010,592 - -1.3 3,010,601 + +0.7 3,010,602 - +0.8 3,010,602 - -1.0 3,010,717 + AMB_RS14185 0.10 -0.8 3,010,717 + AMB_RS14185 0.10 -2.0 3,010,718 - AMB_RS14185 0.10 -3.0 3,010,718 - AMB_RS14185 0.10 -1.8 3,010,945 + AMB_RS14185 0.22 +1.0 3,010,946 - AMB_RS14185 0.22 +1.0 3,010,946 - AMB_RS14185 0.22 -2.0 3,011,036 - AMB_RS14185 0.26 +0.3 3,011,125 + AMB_RS14185 0.30 -2.3 3,011,126 - AMB_RS14185 0.30 -0.5 3,011,201 + AMB_RS14185 0.34 -0.7 3,011,201 + AMB_RS14185 0.34 -2.3 3,012,547 + -0.3 3,012,548 - -1.3 3,012,626 + +0.3 3,012,626 + -2.0 3,012,632 + -3.0 3,012,633 - -0.0 3,012,747 + -1.8 3,012,748 - -4.4 3,012,748 - -0.2 3,012,748 - -0.8 3,012,951 + AMB_RS14190 0.21 -0.4 3,012,951 + AMB_RS14190 0.21 -2.3 3,012,952 - AMB_RS14190 0.21 -3.3 3,012,957 + AMB_RS14190 0.21 -3.7 3,012,957 + AMB_RS14190 0.21 -2.5 3,012,957 + AMB_RS14190 0.21 -1.3 3,012,958 - AMB_RS14190 0.22 -0.1 3,012,958 - AMB_RS14190 0.22 -2.0 3,012,958 - AMB_RS14190 0.22 -3.0 3,013,059 + AMB_RS14190 0.32 -1.3 3,013,060 - AMB_RS14190 0.32 -0.1 3,013,060 - AMB_RS14190 0.32 -1.8 3,013,152 + AMB_RS14190 0.41 -2.5
Or see this region's nucleotide sequence