Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13175 and AMB_RS13180 are separated by 16 nucleotides AMB_RS13180 and AMB_RS13185 overlap by 4 nucleotides AMB_RS13185 and AMB_RS13190 are separated by 68 nucleotides AMB_RS13190 and AMB_RS13195 overlap by 4 nucleotides AMB_RS13195 and AMB_RS13200 overlap by 4 nucleotides
AMB_RS13175: AMB_RS13175 - electron transport complex subunit E, at 2,805,489 to 2,806,160
_RS13175
AMB_RS13180: AMB_RS13180 - protein rnfH, at 2,806,177 to 2,806,470
_RS13180
AMB_RS13185: AMB_RS13185 - hypothetical protein, at 2,806,467 to 2,806,880
_RS13185
AMB_RS13190: AMB_RS13190 - Kef-type K+ transport systems, at 2,806,949 to 2,808,193
_RS13190
AMB_RS13195: AMB_RS13195 - putrescine ABC transporter permease PotI, at 2,808,190 to 2,808,996
_RS13195
AMB_RS13200: AMB_RS13200 - putrescine ABC transporter permease PotH, at 2,808,993 to 2,809,943
_RS13200
Position (kb)
2806
2807
2808
2809 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2806.176 kb on + strand at 2806.176 kb on + strand at 2806.177 kb on - strand at 2806.177 kb on - strand at 2806.177 kb on - strand at 2806.177 kb on - strand at 2806.195 kb on + strand at 2806.196 kb on - strand at 2806.387 kb on + strand, within AMB_RS13180 at 2806.388 kb on - strand, within AMB_RS13180 at 2806.388 kb on - strand, within AMB_RS13180 at 2806.507 kb on - strand at 2806.507 kb on - strand at 2806.518 kb on + strand, within AMB_RS13185 at 2806.518 kb on + strand, within AMB_RS13185 at 2806.519 kb on - strand, within AMB_RS13185 at 2806.565 kb on + strand, within AMB_RS13185 at 2806.565 kb on + strand, within AMB_RS13185 at 2806.566 kb on - strand, within AMB_RS13185 at 2806.749 kb on + strand, within AMB_RS13185 at 2806.946 kb on + strand at 2806.947 kb on - strand at 2808.147 kb on + strand at 2808.147 kb on + strand at 2808.911 kb on + strand, within AMB_RS13195 at 2808.939 kb on - strand at 2808.950 kb on + strand at 2808.969 kb on + strand at 2808.970 kb on - strand at 2808.971 kb on + strand at 2808.971 kb on + strand at 2808.972 kb on - strand at 2808.972 kb on - strand at 2808.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 2,806,176 + -1.2 2,806,176 + -0.7 2,806,177 - -0.5 2,806,177 - -0.5 2,806,177 - -1.2 2,806,177 - -0.4 2,806,195 + -1.0 2,806,196 - -0.2 2,806,387 + AMB_RS13180 0.71 -1.0 2,806,388 - AMB_RS13180 0.72 +0.0 2,806,388 - AMB_RS13180 0.72 -0.5 2,806,507 - -2.3 2,806,507 - +0.2 2,806,518 + AMB_RS13185 0.12 -0.1 2,806,518 + AMB_RS13185 0.12 -0.4 2,806,519 - AMB_RS13185 0.13 +0.4 2,806,565 + AMB_RS13185 0.24 -1.5 2,806,565 + AMB_RS13185 0.24 +0.1 2,806,566 - AMB_RS13185 0.24 -1.3 2,806,749 + AMB_RS13185 0.68 -3.1 2,806,946 + -0.0 2,806,947 - -1.9 2,808,147 + +0.1 2,808,147 + -0.5 2,808,911 + AMB_RS13195 0.89 +0.1 2,808,939 - -0.5 2,808,950 + -2.8 2,808,969 + -2.9 2,808,970 - +0.2 2,808,971 + -2.1 2,808,971 + -1.6 2,808,972 - -1.3 2,808,972 - +0.4 2,808,972 - -0.5
Or see this region's nucleotide sequence