Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11825 and AMB_RS11830 are separated by 18 nucleotides AMB_RS11830 and AMB_RS11835 overlap by 29 nucleotides AMB_RS11835 and AMB_RS11840 are separated by 134 nucleotides AMB_RS11840 and AMB_RS11845 overlap by 4 nucleotides
AMB_RS11825: AMB_RS11825 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,547,457 to 2,548,533
_RS11825
AMB_RS11830: AMB_RS11830 - riboflavin synthase, at 2,548,552 to 2,549,144
_RS11830
AMB_RS11835: AMB_RS11835 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 2,549,116 to 2,550,264
_RS11835
AMB_RS11840: AMB_RS11840 - 6,7-dimethyl-8-ribityllumazine synthase, at 2,550,399 to 2,550,851
_RS11840
AMB_RS11845: AMB_RS11845 - N utilization substance protein B, at 2,550,848 to 2,551,318
_RS11845
Position (kb)
2549
2550
2551 Strain fitness (log2 ratio)
-2
-1
0
1 at 2548.529 kb on + strand at 2548.531 kb on + strand at 2548.531 kb on + strand at 2549.170 kb on + strand at 2550.337 kb on + strand at 2550.338 kb on - strand at 2550.378 kb on + strand at 2550.378 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 2,548,529 + -0.4 2,548,531 + -2.0 2,548,531 + +0.1 2,549,170 + -0.0 2,550,337 + -0.2 2,550,338 - -0.6 2,550,378 + +0.0 2,550,378 + -0.5 2,550,382 + -0.6 2,550,382 + -1.3 2,550,382 + -1.4 2,550,382 + -1.6 2,550,382 + -0.0 2,550,382 + +1.0
Or see this region's nucleotide sequence