Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01725 and AMB_RS01730 are separated by 114 nucleotides AMB_RS01730 and AMB_RS01735 are separated by 385 nucleotides AMB_RS01735 and AMB_RS01740 are separated by 393 nucleotides AMB_RS01740 and AMB_RS01745 are separated by 95 nucleotides
AMB_RS01725: AMB_RS01725 - translation initiation factor IF-3, at 379,794 to 380,315
_RS01725
AMB_RS01730: AMB_RS01730 - tRNA-Ser, at 380,430 to 380,519
_RS01730
AMB_RS01735: AMB_RS01735 - Uncharacterized protein, involved in the regulation of septum location, at 380,905 to 381,195
_RS01735
AMB_RS01740: AMB_RS01740 - hypothetical protein, at 381,589 to 381,834
_RS01740
AMB_RS01745: AMB_RS01745 - hypothetical protein, at 381,930 to 382,334
_RS01745
Position (kb) 380
381
382 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 380.313 kb on + strand at 380.313 kb on + strand at 380.314 kb on - strand at 380.339 kb on - strand at 380.381 kb on + strand at 380.381 kb on + strand at 380.551 kb on - strand at 380.893 kb on - strand at 380.911 kb on - strand at 380.929 kb on - strand at 380.999 kb on + strand, within AMB_RS01735 at 380.999 kb on + strand, within AMB_RS01735 at 380.999 kb on + strand, within AMB_RS01735 at 380.999 kb on + strand, within AMB_RS01735 at 380.999 kb on + strand, within AMB_RS01735 at 380.999 kb on + strand, within AMB_RS01735 at 381.000 kb on - strand, within AMB_RS01735 at 381.000 kb on - strand, within AMB_RS01735 at 381.000 kb on - strand, within AMB_RS01735 at 381.139 kb on - strand, within AMB_RS01735 at 381.139 kb on - strand, within AMB_RS01735 at 381.139 kb on - strand, within AMB_RS01735 at 381.173 kb on + strand at 381.173 kb on + strand at 381.174 kb on - strand at 381.174 kb on - strand at 381.174 kb on - strand at 381.193 kb on + strand at 381.248 kb on + strand at 381.277 kb on + strand at 381.277 kb on + strand at 381.278 kb on - strand at 381.297 kb on + strand at 381.298 kb on - strand at 381.484 kb on + strand at 381.484 kb on + strand at 381.507 kb on + strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.544 kb on + strand at 381.544 kb on + strand at 381.545 kb on - strand at 381.644 kb on + strand, within AMB_RS01740 at 381.644 kb on + strand, within AMB_RS01740 at 381.645 kb on - strand, within AMB_RS01740 at 381.645 kb on - strand, within AMB_RS01740 at 381.752 kb on + strand, within AMB_RS01740 at 381.752 kb on + strand, within AMB_RS01740 at 381.753 kb on - strand, within AMB_RS01740 at 381.753 kb on - strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.896 kb on + strand at 381.896 kb on + strand at 381.897 kb on - strand at 382.075 kb on + strand, within AMB_RS01745 at 382.076 kb on - strand, within AMB_RS01745 at 382.090 kb on + strand, within AMB_RS01745 at 382.090 kb on + strand, within AMB_RS01745
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 380,313 + -1.4 380,313 + -0.1 380,314 - -1.4 380,339 - -1.8 380,381 + -0.7 380,381 + +0.1 380,551 - +1.2 380,893 - +0.0 380,911 - -0.4 380,929 - +0.1 380,999 + AMB_RS01735 0.32 +0.1 380,999 + AMB_RS01735 0.32 -0.1 380,999 + AMB_RS01735 0.32 +0.2 380,999 + AMB_RS01735 0.32 +0.6 380,999 + AMB_RS01735 0.32 +0.3 380,999 + AMB_RS01735 0.32 +0.4 381,000 - AMB_RS01735 0.33 -0.1 381,000 - AMB_RS01735 0.33 -2.1 381,000 - AMB_RS01735 0.33 +0.6 381,139 - AMB_RS01735 0.80 -0.3 381,139 - AMB_RS01735 0.80 -2.1 381,139 - AMB_RS01735 0.80 +0.6 381,173 + -0.1 381,173 + -0.0 381,174 - -1.4 381,174 - -0.3 381,174 - +1.2 381,193 + -0.8 381,248 + -1.6 381,277 + +0.9 381,277 + -1.4 381,278 - -0.8 381,297 + -3.8 381,298 - +0.1 381,484 + +0.2 381,484 + -0.1 381,507 + +0.5 381,508 - -0.2 381,508 - +0.3 381,508 - -0.4 381,508 - -0.0 381,508 - -0.3 381,508 - +0.2 381,508 - +0.2 381,544 + +0.2 381,544 + -0.1 381,545 - -2.6 381,644 + AMB_RS01740 0.22 -0.4 381,644 + AMB_RS01740 0.22 +0.3 381,645 - AMB_RS01740 0.23 -1.1 381,645 - AMB_RS01740 0.23 +1.2 381,752 + AMB_RS01740 0.66 -0.4 381,752 + AMB_RS01740 0.66 +0.4 381,753 - AMB_RS01740 0.67 +1.2 381,753 - AMB_RS01740 0.67 -0.3 381,800 + AMB_RS01740 0.86 -1.4 381,800 + AMB_RS01740 0.86 +0.7 381,800 + AMB_RS01740 0.86 +0.3 381,800 + AMB_RS01740 0.86 +0.2 381,800 + AMB_RS01740 0.86 +0.3 381,800 + AMB_RS01740 0.86 +0.1 381,800 + AMB_RS01740 0.86 -0.4 381,800 + AMB_RS01740 0.86 -2.5 381,800 + AMB_RS01740 0.86 +0.1 381,801 - AMB_RS01740 0.86 -2.4 381,801 - AMB_RS01740 0.86 +1.2 381,801 - AMB_RS01740 0.86 +0.1 381,801 - AMB_RS01740 0.86 -0.1 381,896 + +0.6 381,896 + -1.8 381,897 - -1.4 382,075 + AMB_RS01745 0.36 +0.2 382,076 - AMB_RS01745 0.36 -2.5 382,090 + AMB_RS01745 0.40 -0.6 382,090 + AMB_RS01745 0.40 -1.1
Or see this region's nucleotide sequence