Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01365 and AMB_RS01370 overlap by 26 nucleotides AMB_RS01370 and AMB_RS01375 are separated by 183 nucleotides
AMB_RS01365: AMB_RS01365 - nitroreductase, at 300,479 to 302,077
_RS01365
AMB_RS01370: AMB_RS01370 - hypothetical protein, at 302,052 to 302,240
_RS01370
AMB_RS01375: AMB_RS01375 - peptide chain release factor 3, at 302,424 to 304,010
_RS01375
Position (kb) 302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 301.056 kb on - strand, within AMB_RS01365 at 301.056 kb on - strand, within AMB_RS01365 at 301.267 kb on + strand, within AMB_RS01365 at 301.688 kb on + strand, within AMB_RS01365 at 301.688 kb on + strand, within AMB_RS01365 at 301.689 kb on - strand, within AMB_RS01365 at 301.689 kb on - strand, within AMB_RS01365 at 301.689 kb on - strand, within AMB_RS01365 at 301.689 kb on - strand, within AMB_RS01365 at 301.689 kb on - strand, within AMB_RS01365 at 301.747 kb on + strand, within AMB_RS01365 at 301.747 kb on + strand, within AMB_RS01365 at 301.748 kb on - strand, within AMB_RS01365 at 301.871 kb on + strand, within AMB_RS01365 at 301.871 kb on + strand, within AMB_RS01365 at 301.872 kb on - strand, within AMB_RS01365 at 301.872 kb on - strand, within AMB_RS01365 at 301.872 kb on - strand, within AMB_RS01365 at 301.920 kb on - strand at 301.985 kb on + strand at 301.985 kb on + strand at 301.985 kb on + strand at 301.986 kb on - strand at 302.042 kb on + strand at 302.042 kb on + strand at 302.043 kb on - strand at 302.043 kb on - strand at 302.199 kb on - strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.200 kb on + strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.201 kb on - strand, within AMB_RS01370 at 302.311 kb on + strand at 302.312 kb on - strand at 302.312 kb on - strand at 302.359 kb on + strand at 302.360 kb on - strand at 302.379 kb on + strand at 302.379 kb on + strand at 302.379 kb on + strand at 302.379 kb on + strand at 302.379 kb on + strand at 302.380 kb on - strand at 302.380 kb on - strand at 302.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 301,056 - AMB_RS01365 0.36 -0.3 301,056 - AMB_RS01365 0.36 +1.9 301,267 + AMB_RS01365 0.49 -1.0 301,688 + AMB_RS01365 0.76 -1.1 301,688 + AMB_RS01365 0.76 -0.0 301,689 - AMB_RS01365 0.76 -2.5 301,689 - AMB_RS01365 0.76 -1.4 301,689 - AMB_RS01365 0.76 -1.6 301,689 - AMB_RS01365 0.76 -1.1 301,689 - AMB_RS01365 0.76 -0.1 301,747 + AMB_RS01365 0.79 -0.1 301,747 + AMB_RS01365 0.79 -0.4 301,748 - AMB_RS01365 0.79 +1.2 301,871 + AMB_RS01365 0.87 -0.1 301,871 + AMB_RS01365 0.87 +0.9 301,872 - AMB_RS01365 0.87 +0.2 301,872 - AMB_RS01365 0.87 +0.3 301,872 - AMB_RS01365 0.87 -0.8 301,920 - +1.2 301,985 + -0.2 301,985 + -0.2 301,985 + -1.3 301,986 - -2.3 302,042 + +0.2 302,042 + +0.4 302,043 - -1.6 302,043 - -0.1 302,199 - AMB_RS01370 0.78 -1.1 302,200 + AMB_RS01370 0.78 -1.4 302,200 + AMB_RS01370 0.78 +0.1 302,200 + AMB_RS01370 0.78 -0.2 302,200 + AMB_RS01370 0.78 -0.2 302,200 + AMB_RS01370 0.78 +0.2 302,200 + AMB_RS01370 0.78 -0.2 302,200 + AMB_RS01370 0.78 -2.6 302,200 + AMB_RS01370 0.78 -2.8 302,200 + AMB_RS01370 0.78 -1.4 302,200 + AMB_RS01370 0.78 +0.2 302,201 - AMB_RS01370 0.79 -0.1 302,201 - AMB_RS01370 0.79 -0.4 302,201 - AMB_RS01370 0.79 +0.7 302,201 - AMB_RS01370 0.79 -0.3 302,201 - AMB_RS01370 0.79 +1.8 302,201 - AMB_RS01370 0.79 -0.4 302,311 + -0.8 302,312 - -0.7 302,312 - -0.5 302,359 + -2.0 302,360 - +0.1 302,379 + -1.6 302,379 + -1.6 302,379 + +0.2 302,379 + -0.1 302,379 + -0.0 302,380 - -1.4 302,380 - -1.1 302,424 - +0.2
Or see this region's nucleotide sequence