Experiment: 30_an_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00665 and AMB_RS00670 are separated by 13 nucleotides AMB_RS00670 and AMB_RS00675 are separated by 4 nucleotides AMB_RS00675 and AMB_RS00680 are separated by 4 nucleotides
AMB_RS00665: AMB_RS00665 - gfo/Idh/MocA family oxidoreductase, at 152,387 to 153,427
_RS00665
AMB_RS00670: AMB_RS00670 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 153,441 to 154,208
_RS00670
AMB_RS00675: AMB_RS00675 - KR domain-containing protein, at 154,213 to 154,929
_RS00675
AMB_RS00680: AMB_RS00680 - 3-dehydroquinate synthase, at 154,934 to 155,992
_RS00680
Position (kb) 154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 153.411 kb on + strand at 153.411 kb on + strand at 153.411 kb on + strand at 153.411 kb on + strand at 153.411 kb on + strand at 153.425 kb on + strand at 154.203 kb on + strand at 154.203 kb on + strand at 154.203 kb on + strand at 154.203 kb on + strand at 154.961 kb on + strand at 155.150 kb on + strand, within AMB_RS00680 at 155.150 kb on + strand, within AMB_RS00680 at 155.582 kb on + strand, within AMB_RS00680 at 155.582 kb on + strand, within AMB_RS00680 at 155.582 kb on + strand, within AMB_RS00680 at 155.582 kb on + strand, within AMB_RS00680 at 155.584 kb on + strand, within AMB_RS00680 at 155.795 kb on + strand, within AMB_RS00680
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_1 remove 153,411 + +0.1 153,411 + -0.8 153,411 + +0.1 153,411 + +1.2 153,411 + -1.9 153,425 + +0.5 154,203 + -1.4 154,203 + +0.3 154,203 + -0.4 154,203 + -2.2 154,961 + -1.1 155,150 + AMB_RS00680 0.20 -1.4 155,150 + AMB_RS00680 0.20 -1.6 155,582 + AMB_RS00680 0.61 -0.0 155,582 + AMB_RS00680 0.61 +0.2 155,582 + AMB_RS00680 0.61 +0.6 155,582 + AMB_RS00680 0.61 +0.1 155,584 + AMB_RS00680 0.61 -2.8 155,795 + AMB_RS00680 0.81 -1.6
Or see this region's nucleotide sequence