Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purL and mltF are separated by 257 nucleotides mltF and tadA overlap by 4 nucleotides tadA and yfhB are separated by 57 nucleotides
b2557: purL - phosphoribosylformylglycinamidine synthase (NCBI), at 2,689,678 to 2,693,565
purL
b2558: mltF - predicted periplasmic binding protein/transglycosylase (RefSeq), at 2,693,823 to 2,695,379
mltF
b2559: tadA - tRNA-specific adenosine deaminase (RefSeq), at 2,695,376 to 2,695,879
tadA
b2560: yfhB - orf, hypothetical protein (VIMSS), at 2,695,937 to 2,696,572
yfhB
Position (kb)
2693
2694
2695
2696 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2692.940 kb on + strand, within purL at 2692.973 kb on - strand, within purL at 2693.040 kb on + strand, within purL at 2693.055 kb on + strand, within purL at 2693.288 kb on - strand at 2693.315 kb on + strand at 2693.386 kb on + strand at 2693.577 kb on + strand at 2693.888 kb on - strand at 2693.888 kb on - strand at 2693.916 kb on + strand at 2693.916 kb on + strand at 2694.119 kb on + strand, within mltF at 2694.152 kb on + strand, within mltF at 2694.152 kb on + strand, within mltF at 2694.152 kb on + strand, within mltF at 2694.158 kb on - strand, within mltF at 2694.158 kb on - strand, within mltF at 2694.194 kb on + strand, within mltF at 2694.194 kb on + strand, within mltF at 2694.212 kb on - strand, within mltF at 2694.212 kb on - strand, within mltF at 2694.236 kb on + strand, within mltF at 2694.252 kb on - strand, within mltF at 2694.283 kb on - strand, within mltF at 2694.290 kb on + strand, within mltF at 2694.310 kb on - strand, within mltF at 2694.319 kb on - strand, within mltF at 2694.433 kb on - strand, within mltF at 2694.438 kb on + strand, within mltF at 2694.438 kb on + strand, within mltF at 2694.592 kb on + strand, within mltF at 2694.592 kb on + strand, within mltF at 2694.611 kb on + strand, within mltF at 2694.684 kb on - strand, within mltF at 2694.692 kb on + strand, within mltF at 2694.716 kb on + strand, within mltF at 2694.791 kb on + strand, within mltF at 2694.792 kb on - strand, within mltF at 2694.824 kb on + strand, within mltF at 2694.847 kb on + strand, within mltF at 2694.891 kb on - strand, within mltF at 2694.891 kb on - strand, within mltF at 2694.931 kb on - strand, within mltF at 2694.931 kb on - strand, within mltF at 2694.969 kb on + strand, within mltF at 2694.971 kb on + strand, within mltF at 2694.976 kb on + strand, within mltF at 2695.086 kb on + strand, within mltF at 2695.109 kb on + strand, within mltF at 2695.125 kb on + strand, within mltF at 2695.138 kb on - strand, within mltF at 2695.203 kb on - strand, within mltF at 2695.203 kb on - strand, within mltF at 2695.252 kb on + strand at 2695.252 kb on + strand at 2695.256 kb on - strand at 2695.260 kb on - strand at 2695.328 kb on + strand at 2695.365 kb on - strand at 2695.945 kb on - strand at 2695.945 kb on - strand at 2695.966 kb on + strand at 2695.966 kb on + strand at 2695.968 kb on + strand at 2695.968 kb on + strand at 2696.004 kb on + strand, within yfhB at 2696.004 kb on + strand, within yfhB at 2696.014 kb on + strand, within yfhB at 2696.014 kb on + strand, within yfhB at 2696.024 kb on + strand, within yfhB at 2696.044 kb on + strand, within yfhB at 2696.044 kb on - strand, within yfhB at 2696.050 kb on - strand, within yfhB at 2696.077 kb on - strand, within yfhB at 2696.077 kb on - strand, within yfhB at 2696.089 kb on + strand, within yfhB at 2696.089 kb on + strand, within yfhB at 2696.164 kb on - strand, within yfhB
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 2,692,940 + purL b2557 0.84 +1.0 2,692,973 - purL b2557 0.85 -0.8 2,693,040 + purL b2557 0.86 +0.6 2,693,055 + purL b2557 0.87 +1.5 2,693,288 - +0.2 2,693,315 + +0.7 2,693,386 + -0.8 2,693,577 + -0.7 2,693,888 - -2.8 2,693,888 - -0.2 2,693,916 + -0.5 2,693,916 + -1.3 2,694,119 + mltF b2558 0.19 -0.1 2,694,152 + mltF b2558 0.21 -0.3 2,694,152 + mltF b2558 0.21 -0.3 2,694,152 + mltF b2558 0.21 -1.3 2,694,158 - mltF b2558 0.22 -0.3 2,694,158 - mltF b2558 0.22 -1.4 2,694,194 + mltF b2558 0.24 -1.3 2,694,194 + mltF b2558 0.24 -0.4 2,694,212 - mltF b2558 0.25 -0.3 2,694,212 - mltF b2558 0.25 -1.0 2,694,236 + mltF b2558 0.27 -2.3 2,694,252 - mltF b2558 0.28 +0.3 2,694,283 - mltF b2558 0.30 +0.2 2,694,290 + mltF b2558 0.30 -0.7 2,694,310 - mltF b2558 0.31 -1.4 2,694,319 - mltF b2558 0.32 -0.1 2,694,433 - mltF b2558 0.39 -1.0 2,694,438 + mltF b2558 0.39 +2.0 2,694,438 + mltF b2558 0.39 -0.6 2,694,592 + mltF b2558 0.49 -0.8 2,694,592 + mltF b2558 0.49 -1.6 2,694,611 + mltF b2558 0.51 -0.1 2,694,684 - mltF b2558 0.55 -1.6 2,694,692 + mltF b2558 0.56 -2.1 2,694,716 + mltF b2558 0.57 +1.1 2,694,791 + mltF b2558 0.62 -1.3 2,694,792 - mltF b2558 0.62 -0.3 2,694,824 + mltF b2558 0.64 -0.7 2,694,847 + mltF b2558 0.66 -0.8 2,694,891 - mltF b2558 0.69 -0.3 2,694,891 - mltF b2558 0.69 -2.4 2,694,931 - mltF b2558 0.71 -0.5 2,694,931 - mltF b2558 0.71 -0.4 2,694,969 + mltF b2558 0.74 -1.0 2,694,971 + mltF b2558 0.74 -1.3 2,694,976 + mltF b2558 0.74 -0.7 2,695,086 + mltF b2558 0.81 -0.4 2,695,109 + mltF b2558 0.83 -0.3 2,695,125 + mltF b2558 0.84 -0.1 2,695,138 - mltF b2558 0.84 -0.9 2,695,203 - mltF b2558 0.89 -1.2 2,695,203 - mltF b2558 0.89 -2.4 2,695,252 + +0.4 2,695,252 + -1.3 2,695,256 - -0.2 2,695,260 - -0.4 2,695,328 + -0.7 2,695,365 - -0.7 2,695,945 - -0.3 2,695,945 - +1.4 2,695,966 + +0.0 2,695,966 + +0.1 2,695,968 + -0.6 2,695,968 + +0.7 2,696,004 + yfhB b2560 0.11 -2.0 2,696,004 + yfhB b2560 0.11 +2.0 2,696,014 + yfhB b2560 0.12 -1.1 2,696,014 + yfhB b2560 0.12 -0.1 2,696,024 + yfhB b2560 0.14 +0.4 2,696,044 + yfhB b2560 0.17 +0.5 2,696,044 - yfhB b2560 0.17 +0.2 2,696,050 - yfhB b2560 0.18 +0.3 2,696,077 - yfhB b2560 0.22 -0.7 2,696,077 - yfhB b2560 0.22 -1.2 2,696,089 + yfhB b2560 0.24 +0.9 2,696,089 + yfhB b2560 0.24 +0.2 2,696,164 - yfhB b2560 0.36 -0.8
Or see this region's nucleotide sequence