Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxC and rsxD are separated by 0 nucleotides rsxD and rsxG are separated by 3 nucleotides rsxG and rsxE are separated by 3 nucleotides
b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165
rsxC
b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224
rsxD
b1631: rsxG - electron transport complex protein RnfG (NCBI), at 1,708,228 to 1,708,848
rsxG
b1632: rsxE - NADH-ubiquinone oxidoreductase (NCBI), at 1,708,852 to 1,709,547
rsxE
Position (kb)
1707
1708
1709 Strain fitness (log2 ratio)
-1
0
1 at 1706.363 kb on + strand, within rsxC at 1706.547 kb on - strand, within rsxC at 1706.829 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.859 kb on + strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.883 kb on - strand, within rsxC at 1706.924 kb on + strand, within rsxC at 1707.252 kb on + strand at 1707.252 kb on + strand at 1707.328 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.414 kb on + strand, within rsxD at 1707.473 kb on - strand, within rsxD at 1707.579 kb on - strand, within rsxD at 1707.696 kb on - strand, within rsxD at 1707.716 kb on + strand, within rsxD at 1707.793 kb on + strand, within rsxD at 1707.803 kb on - strand, within rsxD at 1707.895 kb on + strand, within rsxD at 1707.914 kb on - strand, within rsxD at 1707.924 kb on + strand, within rsxD at 1708.021 kb on + strand, within rsxD at 1708.161 kb on + strand at 1708.161 kb on + strand at 1708.187 kb on - strand at 1708.227 kb on - strand at 1708.253 kb on + strand at 1708.253 kb on + strand at 1708.996 kb on - strand, within rsxE at 1709.004 kb on + strand, within rsxE at 1709.034 kb on - strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.106 kb on - strand, within rsxE at 1709.123 kb on + strand, within rsxE at 1709.128 kb on + strand, within rsxE at 1709.182 kb on + strand, within rsxE
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 1,706,363 + rsxC b1629 0.64 +1.3 1,706,547 - rsxC b1629 0.72 +0.5 1,706,829 + rsxC b1629 0.85 -0.1 1,706,859 + rsxC b1629 0.86 -0.3 1,706,859 + rsxC b1629 0.86 +0.1 1,706,883 - rsxC b1629 0.87 -0.1 1,706,883 - rsxC b1629 0.87 +1.0 1,706,924 + rsxC b1629 0.89 -0.1 1,707,252 + +1.3 1,707,252 + -0.7 1,707,328 + rsxD b1630 0.15 +0.8 1,707,414 + rsxD b1630 0.23 +0.3 1,707,414 + rsxD b1630 0.23 +0.4 1,707,473 - rsxD b1630 0.29 -0.2 1,707,579 - rsxD b1630 0.39 -0.1 1,707,696 - rsxD b1630 0.50 +0.3 1,707,716 + rsxD b1630 0.52 -0.1 1,707,793 + rsxD b1630 0.59 +0.6 1,707,803 - rsxD b1630 0.60 +0.8 1,707,895 + rsxD b1630 0.69 -0.7 1,707,914 - rsxD b1630 0.71 +1.0 1,707,924 + rsxD b1630 0.72 -0.4 1,708,021 + rsxD b1630 0.81 +0.1 1,708,161 + -0.7 1,708,161 + +0.1 1,708,187 - -0.7 1,708,227 - +0.3 1,708,253 + -0.1 1,708,253 + +1.7 1,708,996 - rsxE b1632 0.21 +0.0 1,709,004 + rsxE b1632 0.22 -0.5 1,709,034 - rsxE b1632 0.26 -0.5 1,709,096 + rsxE b1632 0.35 +0.3 1,709,096 + rsxE b1632 0.35 -0.7 1,709,106 - rsxE b1632 0.36 -0.5 1,709,123 + rsxE b1632 0.39 -0.6 1,709,128 + rsxE b1632 0.40 +0.0 1,709,182 + rsxE b1632 0.47 -0.1
Or see this region's nucleotide sequence