Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspC and pspD are separated by 8 nucleotides pspD and pspE are separated by 74 nucleotides pspE and ycjM are separated by 212 nucleotides ycjM and ycjN are separated by 13 nucleotides
b1306: pspC - DNA-binding transcriptional activator (NCBI), at 1,367,049 to 1,367,408
pspC
b1307: pspD - peripheral inner membrane phage-shock protein (NCBI), at 1,367,417 to 1,367,638
pspD
b1308: pspE - thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI), at 1,367,713 to 1,368,027
pspE
b1309: ycjM - putative polysaccharide hydrolase (VIMSS), at 1,368,240 to 1,369,919
ycjM
b1310: ycjN - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 1,369,933 to 1,371,225
ycjN
Position (kb)
1368
1369
1370 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1367.384 kb on + strand at 1367.424 kb on + strand at 1367.424 kb on + strand at 1367.427 kb on + strand at 1367.591 kb on + strand, within pspD at 1367.631 kb on - strand at 1367.631 kb on - strand at 1367.795 kb on + strand, within pspE at 1367.795 kb on + strand, within pspE at 1367.813 kb on - strand, within pspE at 1367.842 kb on + strand, within pspE at 1367.852 kb on + strand, within pspE at 1367.860 kb on - strand, within pspE at 1367.932 kb on + strand, within pspE at 1367.941 kb on + strand, within pspE at 1367.990 kb on + strand, within pspE at 1367.991 kb on + strand, within pspE at 1367.991 kb on + strand at 1368.049 kb on - strand at 1368.065 kb on - strand at 1368.090 kb on + strand at 1368.156 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.348 kb on + strand at 1368.432 kb on + strand, within ycjM at 1368.492 kb on + strand, within ycjM at 1368.516 kb on - strand, within ycjM at 1368.517 kb on + strand, within ycjM at 1368.573 kb on + strand, within ycjM at 1368.583 kb on - strand, within ycjM at 1368.594 kb on - strand, within ycjM at 1368.739 kb on - strand, within ycjM at 1368.739 kb on - strand, within ycjM at 1368.766 kb on - strand, within ycjM at 1368.766 kb on - strand, within ycjM at 1368.814 kb on - strand, within ycjM at 1368.822 kb on - strand, within ycjM at 1368.858 kb on - strand, within ycjM at 1368.858 kb on - strand, within ycjM at 1368.880 kb on - strand, within ycjM at 1368.926 kb on - strand, within ycjM at 1368.928 kb on - strand, within ycjM at 1369.030 kb on - strand, within ycjM at 1369.124 kb on + strand, within ycjM at 1369.148 kb on - strand, within ycjM at 1369.164 kb on - strand, within ycjM at 1369.173 kb on + strand, within ycjM at 1369.317 kb on + strand, within ycjM at 1369.317 kb on + strand, within ycjM at 1369.365 kb on + strand, within ycjM at 1369.375 kb on + strand, within ycjM at 1369.414 kb on + strand, within ycjM at 1369.424 kb on + strand, within ycjM at 1369.451 kb on + strand, within ycjM at 1369.451 kb on + strand, within ycjM at 1369.489 kb on + strand, within ycjM at 1369.899 kb on - strand at 1369.976 kb on - strand at 1369.992 kb on - strand at 1370.063 kb on - strand, within ycjN at 1370.064 kb on - strand, within ycjN at 1370.064 kb on - strand, within ycjN at 1370.117 kb on - strand, within ycjN at 1370.173 kb on - strand, within ycjN at 1370.269 kb on + strand, within ycjN at 1370.502 kb on + strand, within ycjN at 1370.527 kb on + strand, within ycjN at 1370.739 kb on - strand, within ycjN at 1370.740 kb on - strand, within ycjN at 1370.811 kb on + strand, within ycjN at 1370.899 kb on - strand, within ycjN at 1370.899 kb on - strand, within ycjN
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 1,367,384 + +0.4 1,367,424 + +0.6 1,367,424 + -0.5 1,367,427 + +0.7 1,367,591 + pspD b1307 0.78 +0.5 1,367,631 - +0.4 1,367,631 - -0.2 1,367,795 + pspE b1308 0.26 +0.4 1,367,795 + pspE b1308 0.26 -1.0 1,367,813 - pspE b1308 0.32 +0.0 1,367,842 + pspE b1308 0.41 +1.0 1,367,852 + pspE b1308 0.44 -0.3 1,367,860 - pspE b1308 0.47 -0.4 1,367,932 + pspE b1308 0.70 +0.3 1,367,941 + pspE b1308 0.72 +0.6 1,367,990 + pspE b1308 0.88 +1.4 1,367,991 + pspE b1308 0.88 -1.6 1,367,991 + +2.4 1,368,049 - -0.6 1,368,065 - +1.1 1,368,090 + -1.6 1,368,156 + +0.4 1,368,323 + -0.0 1,368,323 + +2.0 1,368,323 + -0.5 1,368,348 + +0.6 1,368,432 + ycjM b1309 0.11 +0.9 1,368,492 + ycjM b1309 0.15 -2.0 1,368,516 - ycjM b1309 0.16 -1.1 1,368,517 + ycjM b1309 0.16 +1.4 1,368,573 + ycjM b1309 0.20 -0.0 1,368,583 - ycjM b1309 0.20 -2.8 1,368,594 - ycjM b1309 0.21 -3.1 1,368,739 - ycjM b1309 0.30 +0.0 1,368,739 - ycjM b1309 0.30 -0.6 1,368,766 - ycjM b1309 0.31 +0.2 1,368,766 - ycjM b1309 0.31 +0.5 1,368,814 - ycjM b1309 0.34 -0.0 1,368,822 - ycjM b1309 0.35 -0.6 1,368,858 - ycjM b1309 0.37 +0.0 1,368,858 - ycjM b1309 0.37 -0.0 1,368,880 - ycjM b1309 0.38 -0.2 1,368,926 - ycjM b1309 0.41 +0.2 1,368,928 - ycjM b1309 0.41 +1.4 1,369,030 - ycjM b1309 0.47 -0.3 1,369,124 + ycjM b1309 0.53 +1.1 1,369,148 - ycjM b1309 0.54 +0.0 1,369,164 - ycjM b1309 0.55 +0.1 1,369,173 + ycjM b1309 0.56 +0.4 1,369,317 + ycjM b1309 0.64 +0.3 1,369,317 + ycjM b1309 0.64 -2.8 1,369,365 + ycjM b1309 0.67 -0.5 1,369,375 + ycjM b1309 0.68 +0.1 1,369,414 + ycjM b1309 0.70 +0.6 1,369,424 + ycjM b1309 0.70 +0.9 1,369,451 + ycjM b1309 0.72 -0.1 1,369,451 + ycjM b1309 0.72 -0.8 1,369,489 + ycjM b1309 0.74 -0.8 1,369,899 - +2.2 1,369,976 - +0.9 1,369,992 - +0.1 1,370,063 - ycjN b1310 0.10 -0.4 1,370,064 - ycjN b1310 0.10 -0.2 1,370,064 - ycjN b1310 0.10 -0.1 1,370,117 - ycjN b1310 0.14 -0.3 1,370,173 - ycjN b1310 0.19 -1.1 1,370,269 + ycjN b1310 0.26 -0.3 1,370,502 + ycjN b1310 0.44 +0.0 1,370,527 + ycjN b1310 0.46 -0.8 1,370,739 - ycjN b1310 0.62 -0.6 1,370,740 - ycjN b1310 0.62 +0.7 1,370,811 + ycjN b1310 0.68 +0.2 1,370,899 - ycjN b1310 0.75 +0.4 1,370,899 - ycjN b1310 0.75 -1.2
Or see this region's nucleotide sequence