Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rumB and artJ are separated by 198 nucleotides artJ and artM are separated by 290 nucleotides artM and artQ overlap by 1 nucleotides
b0859: rumB - 23S rRNA m(5)U747-methyltransferase (NCBI), at 897,741 to 898,868
rumB
b0860: artJ - arginine transporter subunit (NCBI), at 899,067 to 899,798
artJ
b0861: artM - arginine transporter subunit (NCBI), at 900,089 to 900,757
artM
b0862: artQ - arginine transporter subunit (NCBI), at 900,757 to 901,473
artQ
Position (kb) 899
900 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 898.164 kb on - strand, within rumB at 898.193 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.201 kb on - strand, within rumB at 898.293 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.331 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.455 kb on + strand, within rumB at 898.468 kb on + strand, within rumB at 898.468 kb on - strand, within rumB at 898.482 kb on + strand, within rumB at 898.490 kb on - strand, within rumB at 898.490 kb on - strand, within rumB at 898.497 kb on + strand, within rumB at 898.498 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.549 kb on + strand, within rumB at 898.700 kb on + strand, within rumB at 898.700 kb on + strand, within rumB at 898.737 kb on + strand, within rumB at 898.827 kb on + strand at 898.827 kb on + strand at 898.946 kb on - strand at 898.946 kb on - strand at 899.181 kb on + strand, within artJ at 899.189 kb on - strand, within artJ at 899.189 kb on - strand, within artJ at 899.335 kb on + strand, within artJ at 899.487 kb on - strand, within artJ at 899.497 kb on + strand, within artJ at 899.497 kb on + strand, within artJ at 899.515 kb on - strand, within artJ at 899.520 kb on - strand, within artJ at 899.522 kb on + strand, within artJ at 899.522 kb on + strand, within artJ at 899.684 kb on + strand, within artJ at 899.846 kb on - strand at 900.015 kb on + strand at 900.140 kb on - strand at 900.140 kb on - strand at 900.193 kb on + strand, within artM at 900.258 kb on - strand, within artM at 900.266 kb on - strand, within artM at 900.270 kb on - strand, within artM at 900.330 kb on - strand, within artM at 900.330 kb on - strand, within artM at 900.445 kb on + strand, within artM at 900.596 kb on - strand, within artM at 900.596 kb on - strand, within artM at 900.611 kb on - strand, within artM at 900.611 kb on - strand, within artM at 900.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 898,164 - rumB b0859 0.38 -0.2 898,193 - rumB b0859 0.40 -0.9 898,201 - rumB b0859 0.41 -0.6 898,201 - rumB b0859 0.41 -0.1 898,293 + rumB b0859 0.49 -0.6 898,331 + rumB b0859 0.52 -0.0 898,331 + rumB b0859 0.52 -0.8 898,455 + rumB b0859 0.63 -0.5 898,455 + rumB b0859 0.63 +0.1 898,468 + rumB b0859 0.64 -0.8 898,468 - rumB b0859 0.64 -2.0 898,482 + rumB b0859 0.66 -1.3 898,490 - rumB b0859 0.66 -0.7 898,490 - rumB b0859 0.66 +0.1 898,497 + rumB b0859 0.67 +0.4 898,498 + rumB b0859 0.67 +0.7 898,549 + rumB b0859 0.72 -0.0 898,549 + rumB b0859 0.72 -0.5 898,549 + rumB b0859 0.72 +0.2 898,700 + rumB b0859 0.85 -1.8 898,700 + rumB b0859 0.85 +0.6 898,737 + rumB b0859 0.88 -0.1 898,827 + -0.6 898,827 + +0.2 898,946 - +3.9 898,946 - -0.3 899,181 + artJ b0860 0.16 -2.5 899,189 - artJ b0860 0.17 +0.3 899,189 - artJ b0860 0.17 +1.0 899,335 + artJ b0860 0.37 +1.0 899,487 - artJ b0860 0.57 +1.3 899,497 + artJ b0860 0.59 -0.9 899,497 + artJ b0860 0.59 +0.0 899,515 - artJ b0860 0.61 +2.5 899,520 - artJ b0860 0.62 +0.3 899,522 + artJ b0860 0.62 +0.4 899,522 + artJ b0860 0.62 +0.5 899,684 + artJ b0860 0.84 +1.0 899,846 - +0.5 900,015 + -0.2 900,140 - +1.1 900,140 - +0.4 900,193 + artM b0861 0.16 -0.5 900,258 - artM b0861 0.25 +0.2 900,266 - artM b0861 0.26 -0.6 900,270 - artM b0861 0.27 -0.5 900,330 - artM b0861 0.36 +1.0 900,330 - artM b0861 0.36 +0.6 900,445 + artM b0861 0.53 +0.0 900,596 - artM b0861 0.76 -0.5 900,596 - artM b0861 0.76 +0.2 900,611 - artM b0861 0.78 +0.2 900,611 - artM b0861 0.78 +1.3 900,706 + -0.0
Or see this region's nucleotide sequence