Experiment: Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgI and ybgJ are separated by 22 nucleotides ybgJ and ybgK overlap by 7 nucleotides ybgK and ybgL overlap by 11 nucleotides ybgL and nei are separated by 35 nucleotides
b0710: ybgI - conserved metal-binding protein (NCBI), at 742,050 to 742,793
ybgI
b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472
ybgJ
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
Position (kb) 743
744
745 Strain fitness (log2 ratio) -2
-1
0
1
2 at 742.541 kb on + strand, within ybgI at 742.541 kb on + strand, within ybgI at 742.615 kb on + strand, within ybgI at 742.699 kb on - strand, within ybgI at 742.753 kb on + strand at 742.761 kb on - strand at 742.761 kb on - strand at 742.973 kb on - strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.091 kb on + strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.185 kb on - strand, within ybgJ at 743.248 kb on - strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.250 kb on + strand, within ybgJ at 743.302 kb on + strand, within ybgJ at 743.369 kb on + strand, within ybgJ at 743.374 kb on + strand, within ybgJ at 743.461 kb on + strand at 743.489 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.500 kb on - strand at 743.500 kb on - strand at 743.504 kb on + strand at 743.515 kb on - strand at 743.538 kb on + strand at 743.538 kb on + strand at 743.539 kb on + strand at 743.539 kb on + strand at 743.595 kb on - strand, within ybgK at 743.595 kb on - strand, within ybgK at 743.642 kb on + strand, within ybgK at 743.799 kb on + strand, within ybgK at 743.865 kb on - strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.888 kb on - strand, within ybgK at 743.920 kb on - strand, within ybgK at 743.946 kb on - strand, within ybgK at 743.956 kb on + strand, within ybgK at 743.956 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.152 kb on + strand, within ybgK at 744.271 kb on - strand, within ybgK at 744.423 kb on - strand at 744.662 kb on - strand, within ybgL at 744.662 kb on - strand, within ybgL at 744.711 kb on + strand, within ybgL at 744.766 kb on + strand, within ybgL at 744.832 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.5 mg/ml remove 742,541 + ybgI b0710 0.66 -0.4 742,541 + ybgI b0710 0.66 +0.6 742,615 + ybgI b0710 0.76 +0.6 742,699 - ybgI b0710 0.87 +0.4 742,753 + -1.6 742,761 - -2.3 742,761 - +1.6 742,973 - ybgJ b0711 0.24 +0.2 743,091 + ybgJ b0711 0.42 +0.9 743,091 + ybgJ b0711 0.42 +0.0 743,091 + ybgJ b0711 0.42 -0.6 743,185 - ybgJ b0711 0.56 +0.6 743,185 - ybgJ b0711 0.56 +0.1 743,185 - ybgJ b0711 0.56 +0.2 743,248 - ybgJ b0711 0.66 -0.4 743,250 + ybgJ b0711 0.66 -2.2 743,250 + ybgJ b0711 0.66 +1.3 743,302 + ybgJ b0711 0.74 +0.6 743,369 + ybgJ b0711 0.84 +1.0 743,374 + ybgJ b0711 0.85 +1.8 743,461 + -0.4 743,489 + -0.4 743,492 + +0.4 743,492 + +1.0 743,492 + -0.7 743,500 - -0.3 743,500 - +1.0 743,504 + +0.6 743,515 - +0.1 743,538 + +0.6 743,538 + +0.0 743,539 + +0.6 743,539 + -2.4 743,595 - ybgK b0712 0.14 +1.9 743,595 - ybgK b0712 0.14 +1.0 743,642 + ybgK b0712 0.19 +0.0 743,799 + ybgK b0712 0.36 +0.5 743,865 - ybgK b0712 0.43 -1.6 743,880 + ybgK b0712 0.44 +0.5 743,880 + ybgK b0712 0.44 -0.7 743,888 - ybgK b0712 0.45 +1.2 743,920 - ybgK b0712 0.49 +1.6 743,946 - ybgK b0712 0.51 +0.1 743,956 + ybgK b0712 0.53 +1.2 743,956 + ybgK b0712 0.53 +1.2 744,039 + ybgK b0712 0.61 -1.4 744,039 + ybgK b0712 0.61 +1.0 744,080 + ybgK b0712 0.66 +0.8 744,080 + ybgK b0712 0.66 +1.6 744,152 + ybgK b0712 0.74 +1.0 744,271 - ybgK b0712 0.86 -0.2 744,423 - +0.9 744,662 - ybgL b0713 0.37 +1.2 744,662 - ybgL b0713 0.37 +0.4 744,711 + ybgL b0713 0.44 +0.6 744,766 + ybgL b0713 0.51 +1.6 744,832 - ybgL b0713 0.60 -0.3 744,978 - ybgL b0713 0.80 +0.4 744,978 - ybgL b0713 0.80 +0.6 745,140 - -1.0 745,148 + -0.4 745,194 + +0.6 745,194 + +0.3 745,286 + nei b0714 0.16 -0.7 745,297 + nei b0714 0.18 +0.2 745,297 + nei b0714 0.18 +1.1 745,358 - nei b0714 0.25 +0.7 745,358 - nei b0714 0.25 +1.0
Or see this region's nucleotide sequence