Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23305 and AMB_RS07640 overlap by 4 nucleotides AMB_RS07640 and AMB_RS07645 overlap by 1 nucleotides AMB_RS07645 and AMB_RS24260 overlap by 4 nucleotides AMB_RS24260 and AMB_RS23315 are separated by 746 nucleotides
AMB_RS23305: AMB_RS23305 - hypothetical protein, at 1,645,005 to 1,645,634
_RS23305
AMB_RS07640: AMB_RS07640 - hypothetical protein, at 1,645,631 to 1,645,930
_RS07640
AMB_RS07645: AMB_RS07645 - hypothetical protein, at 1,645,930 to 1,646,199
_RS07645
AMB_RS24260: AMB_RS24260 - DNA primase, at 1,646,196 to 1,647,494
_RS24260
AMB_RS23315: AMB_RS23315 - hypothetical protein, at 1,648,241 to 1,649,779
_RS23315
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1645.363 kb on - strand, within AMB_RS23305 at 1645.363 kb on - strand, within AMB_RS23305 at 1645.363 kb on - strand, within AMB_RS23305 at 1645.447 kb on - strand, within AMB_RS23305 at 1645.746 kb on - strand, within AMB_RS07640 at 1645.914 kb on - strand at 1645.914 kb on - strand at 1645.914 kb on - strand at 1646.065 kb on + strand, within AMB_RS07645 at 1646.065 kb on + strand, within AMB_RS07645 at 1646.065 kb on + strand, within AMB_RS07645 at 1646.066 kb on - strand, within AMB_RS07645 at 1646.066 kb on - strand, within AMB_RS07645 at 1646.173 kb on - strand at 1646.173 kb on - strand at 1646.294 kb on + strand at 1646.529 kb on + strand, within AMB_RS24260 at 1646.529 kb on + strand, within AMB_RS24260 at 1646.529 kb on + strand, within AMB_RS24260 at 1646.530 kb on - strand, within AMB_RS24260 at 1646.530 kb on - strand, within AMB_RS24260 at 1646.530 kb on - strand, within AMB_RS24260 at 1646.573 kb on + strand, within AMB_RS24260 at 1646.573 kb on + strand, within AMB_RS24260 at 1646.573 kb on + strand, within AMB_RS24260 at 1646.574 kb on - strand, within AMB_RS24260 at 1646.607 kb on + strand, within AMB_RS24260 at 1646.607 kb on + strand, within AMB_RS24260 at 1646.607 kb on + strand, within AMB_RS24260 at 1646.607 kb on + strand, within AMB_RS24260 at 1646.608 kb on - strand, within AMB_RS24260 at 1646.608 kb on - strand, within AMB_RS24260 at 1646.608 kb on - strand, within AMB_RS24260 at 1646.608 kb on - strand, within AMB_RS24260 at 1646.874 kb on + strand, within AMB_RS24260 at 1646.922 kb on + strand, within AMB_RS24260 at 1646.922 kb on + strand, within AMB_RS24260 at 1646.923 kb on - strand, within AMB_RS24260 at 1646.923 kb on - strand, within AMB_RS24260 at 1646.923 kb on - strand, within AMB_RS24260 at 1646.985 kb on + strand, within AMB_RS24260 at 1646.985 kb on + strand, within AMB_RS24260 at 1646.986 kb on - strand, within AMB_RS24260 at 1647.024 kb on + strand, within AMB_RS24260 at 1647.024 kb on + strand, within AMB_RS24260 at 1647.025 kb on - strand, within AMB_RS24260 at 1647.414 kb on + strand at 1647.414 kb on + strand at 1647.414 kb on + strand at 1647.415 kb on - strand at 1647.459 kb on + strand at 1647.459 kb on + strand at 1647.460 kb on - strand at 1647.460 kb on - strand at 1647.662 kb on - strand at 1647.697 kb on + strand at 1647.697 kb on + strand at 1647.698 kb on - strand at 1647.698 kb on - strand at 1647.815 kb on + strand at 1648.092 kb on + strand at 1648.092 kb on + strand at 1648.092 kb on + strand at 1648.092 kb on + strand at 1648.093 kb on - strand at 1648.093 kb on - strand at 1648.093 kb on - strand at 1648.093 kb on - strand at 1648.246 kb on + strand at 1648.246 kb on + strand at 1648.247 kb on - strand at 1648.247 kb on - strand at 1648.427 kb on - strand, within AMB_RS23315
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,645,363 - AMB_RS23305 0.57 +0.1 1,645,363 - AMB_RS23305 0.57 -0.5 1,645,363 - AMB_RS23305 0.57 +0.0 1,645,447 - AMB_RS23305 0.70 +0.1 1,645,746 - AMB_RS07640 0.38 -0.2 1,645,914 - -2.7 1,645,914 - -2.1 1,645,914 - -0.9 1,646,065 + AMB_RS07645 0.50 -2.3 1,646,065 + AMB_RS07645 0.50 -2.8 1,646,065 + AMB_RS07645 0.50 -1.7 1,646,066 - AMB_RS07645 0.50 -2.5 1,646,066 - AMB_RS07645 0.50 +0.1 1,646,173 - -1.7 1,646,173 - -0.1 1,646,294 + -0.1 1,646,529 + AMB_RS24260 0.26 +0.3 1,646,529 + AMB_RS24260 0.26 -2.0 1,646,529 + AMB_RS24260 0.26 -1.0 1,646,530 - AMB_RS24260 0.26 -0.0 1,646,530 - AMB_RS24260 0.26 -1.7 1,646,530 - AMB_RS24260 0.26 -0.2 1,646,573 + AMB_RS24260 0.29 +0.2 1,646,573 + AMB_RS24260 0.29 +0.4 1,646,573 + AMB_RS24260 0.29 -1.0 1,646,574 - AMB_RS24260 0.29 -1.3 1,646,607 + AMB_RS24260 0.32 +0.0 1,646,607 + AMB_RS24260 0.32 -0.7 1,646,607 + AMB_RS24260 0.32 -0.1 1,646,607 + AMB_RS24260 0.32 -0.4 1,646,608 - AMB_RS24260 0.32 -1.3 1,646,608 - AMB_RS24260 0.32 +0.4 1,646,608 - AMB_RS24260 0.32 -0.8 1,646,608 - AMB_RS24260 0.32 -1.7 1,646,874 + AMB_RS24260 0.52 -1.5 1,646,922 + AMB_RS24260 0.56 -1.5 1,646,922 + AMB_RS24260 0.56 +0.3 1,646,923 - AMB_RS24260 0.56 +0.4 1,646,923 - AMB_RS24260 0.56 -0.7 1,646,923 - AMB_RS24260 0.56 -2.5 1,646,985 + AMB_RS24260 0.61 +0.9 1,646,985 + AMB_RS24260 0.61 +0.3 1,646,986 - AMB_RS24260 0.61 -1.3 1,647,024 + AMB_RS24260 0.64 +0.0 1,647,024 + AMB_RS24260 0.64 +0.9 1,647,025 - AMB_RS24260 0.64 +0.4 1,647,414 + -1.5 1,647,414 + -0.9 1,647,414 + -1.9 1,647,415 - +0.3 1,647,459 + -0.1 1,647,459 + -1.8 1,647,460 - +0.1 1,647,460 - -0.4 1,647,662 - +0.6 1,647,697 + -0.3 1,647,697 + +0.2 1,647,698 - -0.7 1,647,698 - -0.8 1,647,815 + -1.0 1,648,092 + -1.8 1,648,092 + +0.6 1,648,092 + -0.3 1,648,092 + -0.2 1,648,093 - -0.1 1,648,093 - +0.1 1,648,093 - -0.7 1,648,093 - +0.3 1,648,246 + +0.1 1,648,246 + -1.0 1,648,247 - +0.1 1,648,247 - -0.7 1,648,427 - AMB_RS23315 0.12 -2.5
Or see this region's nucleotide sequence