Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14155 and AMB_RS14160 overlap by 4 nucleotides AMB_RS14160 and AMB_RS23470 are separated by 318 nucleotides AMB_RS23470 and AMB_RS14170 are separated by 119 nucleotides AMB_RS14170 and AMB_RS14175 are separated by 0 nucleotides
AMB_RS14155: AMB_RS14155 - hypothetical protein, at 3,006,054 to 3,006,362
_RS14155
AMB_RS14160: AMB_RS14160 - RNA polymerase sigma factor, at 3,006,359 to 3,007,024
_RS14160
AMB_RS23470: AMB_RS23470 - hypothetical protein, at 3,007,343 to 3,008,341
_RS23470
AMB_RS14170: AMB_RS14170 - suppressor protein SRP40, at 3,008,461 to 3,009,306
_RS14170
AMB_RS14175: AMB_RS14175 - COQ9 family protein, at 3,009,307 to 3,009,948
_RS14175
Position (kb)
3007
3008
3009 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3006.505 kb on - strand, within AMB_RS14160 at 3006.508 kb on - strand, within AMB_RS14160 at 3006.508 kb on - strand, within AMB_RS14160 at 3006.509 kb on + strand, within AMB_RS14160 at 3006.509 kb on + strand, within AMB_RS14160 at 3006.510 kb on - strand, within AMB_RS14160 at 3006.510 kb on - strand, within AMB_RS14160 at 3006.697 kb on - strand, within AMB_RS14160 at 3007.128 kb on + strand at 3007.513 kb on + strand, within AMB_RS23470 at 3007.945 kb on - strand, within AMB_RS23470 at 3007.945 kb on - strand, within AMB_RS23470 at 3007.945 kb on - strand, within AMB_RS23470 at 3008.099 kb on + strand, within AMB_RS23470 at 3008.291 kb on + strand at 3008.292 kb on - strand at 3008.302 kb on + strand at 3008.302 kb on + strand at 3008.324 kb on - strand at 3008.324 kb on - strand at 3008.439 kb on + strand at 3008.439 kb on + strand at 3008.439 kb on + strand at 3008.748 kb on + strand, within AMB_RS14170 at 3008.859 kb on + strand, within AMB_RS14170 at 3008.860 kb on - strand, within AMB_RS14170 at 3008.860 kb on - strand, within AMB_RS14170 at 3008.890 kb on + strand, within AMB_RS14170 at 3008.890 kb on + strand, within AMB_RS14170 at 3009.133 kb on + strand, within AMB_RS14170 at 3009.134 kb on - strand, within AMB_RS14170 at 3009.166 kb on + strand, within AMB_RS14170 at 3009.166 kb on + strand, within AMB_RS14170 at 3009.166 kb on + strand, within AMB_RS14170 at 3009.167 kb on - strand, within AMB_RS14170 at 3009.167 kb on - strand, within AMB_RS14170 at 3009.167 kb on - strand, within AMB_RS14170 at 3009.207 kb on + strand, within AMB_RS14170 at 3009.208 kb on - strand, within AMB_RS14170 at 3009.259 kb on + strand at 3009.259 kb on + strand at 3009.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,006,505 - AMB_RS14160 0.22 -1.2 3,006,508 - AMB_RS14160 0.22 -0.0 3,006,508 - AMB_RS14160 0.22 +0.3 3,006,509 + AMB_RS14160 0.23 +0.1 3,006,509 + AMB_RS14160 0.23 -0.3 3,006,510 - AMB_RS14160 0.23 -0.9 3,006,510 - AMB_RS14160 0.23 -4.3 3,006,697 - AMB_RS14160 0.51 +0.4 3,007,128 + -1.4 3,007,513 + AMB_RS23470 0.17 +0.1 3,007,945 - AMB_RS23470 0.60 -0.7 3,007,945 - AMB_RS23470 0.60 -2.1 3,007,945 - AMB_RS23470 0.60 -1.4 3,008,099 + AMB_RS23470 0.76 -1.9 3,008,291 + -0.0 3,008,292 - -0.1 3,008,302 + -0.1 3,008,302 + -1.9 3,008,324 - -1.3 3,008,324 - +0.2 3,008,439 + -0.1 3,008,439 + -0.1 3,008,439 + -0.4 3,008,748 + AMB_RS14170 0.34 +0.3 3,008,859 + AMB_RS14170 0.47 +0.4 3,008,860 - AMB_RS14170 0.47 -0.2 3,008,860 - AMB_RS14170 0.47 +1.4 3,008,890 + AMB_RS14170 0.51 +0.2 3,008,890 + AMB_RS14170 0.51 +0.3 3,009,133 + AMB_RS14170 0.79 -0.6 3,009,134 - AMB_RS14170 0.80 -0.7 3,009,166 + AMB_RS14170 0.83 +0.1 3,009,166 + AMB_RS14170 0.83 +3.1 3,009,166 + AMB_RS14170 0.83 +0.7 3,009,167 - AMB_RS14170 0.83 +0.6 3,009,167 - AMB_RS14170 0.83 +0.1 3,009,167 - AMB_RS14170 0.83 +0.1 3,009,207 + AMB_RS14170 0.88 -0.6 3,009,208 - AMB_RS14170 0.88 -0.6 3,009,259 + -0.1 3,009,259 + +0.4 3,009,260 - -1.4
Or see this region's nucleotide sequence