Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23715 and AMB_RS23195 overlap by 4 nucleotides AMB_RS23195 and AMB_RS05435 overlap by 4 nucleotides AMB_RS05435 and AMB_RS05440 are separated by 129 nucleotides
AMB_RS23715: AMB_RS23715 - nodulation protein noeE, at 1,123,029 to 1,124,330
_RS23715
AMB_RS23195: AMB_RS23195 - ABC transporter ATP-binding protein, at 1,124,327 to 1,125,610
_RS23195
AMB_RS05435: AMB_RS05435 - ABC transporter permease, at 1,125,607 to 1,126,395
_RS05435
AMB_RS05440: AMB_RS05440 - hypothetical protein, at 1,126,525 to 1,127,946
_RS05440
Position (kb) 1124
1125
1126 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1123.435 kb on - strand, within AMB_RS23715 at 1123.608 kb on - strand, within AMB_RS23715 at 1124.014 kb on + strand, within AMB_RS23715 at 1124.071 kb on + strand, within AMB_RS23715 at 1124.337 kb on - strand at 1124.337 kb on - strand at 1124.472 kb on + strand, within AMB_RS23195 at 1124.473 kb on - strand, within AMB_RS23195 at 1124.655 kb on + strand, within AMB_RS23195 at 1124.691 kb on - strand, within AMB_RS23195 at 1124.863 kb on + strand, within AMB_RS23195 at 1124.866 kb on - strand, within AMB_RS23195 at 1124.997 kb on + strand, within AMB_RS23195 at 1124.998 kb on - strand, within AMB_RS23195 at 1125.001 kb on + strand, within AMB_RS23195 at 1125.001 kb on + strand, within AMB_RS23195 at 1125.002 kb on - strand, within AMB_RS23195 at 1125.139 kb on - strand, within AMB_RS23195 at 1125.528 kb on + strand at 1125.611 kb on - strand at 1125.820 kb on + strand, within AMB_RS05435 at 1125.821 kb on - strand, within AMB_RS05435 at 1125.923 kb on + strand, within AMB_RS05435 at 1125.923 kb on + strand, within AMB_RS05435 at 1125.923 kb on + strand, within AMB_RS05435 at 1125.924 kb on - strand, within AMB_RS05435 at 1125.924 kb on - strand, within AMB_RS05435 at 1126.076 kb on + strand, within AMB_RS05435 at 1126.395 kb on + strand at 1126.396 kb on - strand at 1126.445 kb on - strand at 1126.474 kb on - strand at 1126.474 kb on - strand at 1126.474 kb on - strand at 1126.474 kb on - strand at 1126.474 kb on - strand at 1126.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 1,123,435 - AMB_RS23715 0.31 -2.2 1,123,608 - AMB_RS23715 0.44 -0.9 1,124,014 + AMB_RS23715 0.76 -3.0 1,124,071 + AMB_RS23715 0.80 -1.6 1,124,337 - +0.1 1,124,337 - +0.2 1,124,472 + AMB_RS23195 0.11 -4.5 1,124,473 - AMB_RS23195 0.11 -1.2 1,124,655 + AMB_RS23195 0.26 -2.0 1,124,691 - AMB_RS23195 0.28 -2.6 1,124,863 + AMB_RS23195 0.42 -6.2 1,124,866 - AMB_RS23195 0.42 -1.8 1,124,997 + AMB_RS23195 0.52 -1.0 1,124,998 - AMB_RS23195 0.52 -0.2 1,125,001 + AMB_RS23195 0.52 -1.5 1,125,001 + AMB_RS23195 0.52 -1.6 1,125,002 - AMB_RS23195 0.53 -0.8 1,125,139 - AMB_RS23195 0.63 -1.4 1,125,528 + -1.4 1,125,611 - -3.7 1,125,820 + AMB_RS05435 0.27 -1.6 1,125,821 - AMB_RS05435 0.27 -3.5 1,125,923 + AMB_RS05435 0.40 -2.2 1,125,923 + AMB_RS05435 0.40 -4.4 1,125,923 + AMB_RS05435 0.40 -1.6 1,125,924 - AMB_RS05435 0.40 -0.2 1,125,924 - AMB_RS05435 0.40 -0.9 1,126,076 + AMB_RS05435 0.59 -1.8 1,126,395 + -2.2 1,126,396 - +0.3 1,126,445 - -2.4 1,126,474 - -1.2 1,126,474 - +0.2 1,126,474 - -1.0 1,126,474 - -0.9 1,126,474 - -0.3 1,126,573 - +0.6
Or see this region's nucleotide sequence