Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22845 and AMB_RS22850 are separated by 357 nucleotides AMB_RS22850 and AMB_RS22855 are separated by 77 nucleotides AMB_RS22855 and AMB_RS22860 are separated by 365 nucleotides
AMB_RS22845: AMB_RS22845 - hypothetical protein, at 4,931,318 to 4,931,506
_RS22845
AMB_RS22850: AMB_RS22850 - hypothetical protein, at 4,931,864 to 4,932,235
_RS22850
AMB_RS22855: AMB_RS22855 - phospholipase, at 4,932,313 to 4,932,737
_RS22855
AMB_RS22860: AMB_RS22860 - hypothetical protein, at 4,933,103 to 4,933,876
_RS22860
Position (kb)
4932
4933 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4931.597 kb on - strand at 4931.812 kb on - strand at 4931.865 kb on + strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.950 kb on + strand, within AMB_RS22850 at 4931.950 kb on + strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.952 kb on + strand, within AMB_RS22850 at 4931.953 kb on - strand, within AMB_RS22850 at 4931.997 kb on + strand, within AMB_RS22850 at 4931.998 kb on - strand, within AMB_RS22850 at 4932.039 kb on + strand, within AMB_RS22850 at 4932.040 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.092 kb on - strand, within AMB_RS22850 at 4932.209 kb on + strand at 4932.210 kb on - strand at 4932.240 kb on + strand at 4932.240 kb on + strand at 4932.249 kb on + strand at 4932.250 kb on - strand at 4932.289 kb on + strand at 4932.368 kb on + strand, within AMB_RS22855 at 4932.369 kb on - strand, within AMB_RS22855 at 4932.369 kb on - strand, within AMB_RS22855 at 4932.369 kb on - strand, within AMB_RS22855 at 4932.583 kb on + strand, within AMB_RS22855 at 4932.583 kb on + strand, within AMB_RS22855 at 4932.584 kb on - strand, within AMB_RS22855 at 4932.842 kb on + strand at 4932.842 kb on + strand at 4932.842 kb on + strand at 4932.843 kb on - strand at 4932.843 kb on - strand at 4932.845 kb on - strand at 4932.850 kb on + strand at 4932.850 kb on + strand at 4932.850 kb on + strand at 4932.850 kb on + strand at 4932.851 kb on - strand at 4932.862 kb on + strand at 4932.863 kb on - strand at 4932.863 kb on - strand at 4932.863 kb on - strand at 4932.908 kb on - strand at 4932.908 kb on - strand at 4932.952 kb on + strand at 4932.952 kb on + strand at 4932.952 kb on + strand at 4932.953 kb on - strand at 4932.955 kb on + strand at 4933.007 kb on + strand at 4933.050 kb on + strand at 4933.051 kb on - strand at 4933.051 kb on - strand at 4933.059 kb on + strand at 4933.060 kb on - strand at 4933.346 kb on + strand, within AMB_RS22860 at 4933.347 kb on - strand, within AMB_RS22860 at 4933.439 kb on + strand, within AMB_RS22860 at 4933.439 kb on + strand, within AMB_RS22860 at 4933.439 kb on + strand, within AMB_RS22860 at 4933.439 kb on + strand, within AMB_RS22860 at 4933.439 kb on + strand, within AMB_RS22860 at 4933.440 kb on - strand, within AMB_RS22860 at 4933.440 kb on - strand, within AMB_RS22860 at 4933.440 kb on - strand, within AMB_RS22860 at 4933.457 kb on + strand, within AMB_RS22860 at 4933.458 kb on - strand, within AMB_RS22860 at 4933.458 kb on - strand, within AMB_RS22860 at 4933.541 kb on + strand, within AMB_RS22860
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,931,597 - -0.8 4,931,812 - -1.1 4,931,865 + -1.7 4,931,866 - +0.6 4,931,866 - -0.1 4,931,866 - -2.6 4,931,866 - -0.5 4,931,866 - -0.1 4,931,866 - -1.6 4,931,950 + AMB_RS22850 0.23 +0.7 4,931,950 + AMB_RS22850 0.23 -0.4 4,931,951 - AMB_RS22850 0.23 +0.1 4,931,951 - AMB_RS22850 0.23 -0.1 4,931,951 - AMB_RS22850 0.23 +0.2 4,931,951 - AMB_RS22850 0.23 -0.2 4,931,952 + AMB_RS22850 0.24 -1.7 4,931,953 - AMB_RS22850 0.24 -0.7 4,931,997 + AMB_RS22850 0.36 +0.6 4,931,998 - AMB_RS22850 0.36 +0.6 4,932,039 + AMB_RS22850 0.47 +0.1 4,932,040 - AMB_RS22850 0.47 +0.6 4,932,044 - AMB_RS22850 0.48 +0.6 4,932,044 - AMB_RS22850 0.48 +0.0 4,932,044 - AMB_RS22850 0.48 -0.2 4,932,092 - AMB_RS22850 0.61 -0.2 4,932,209 + -1.0 4,932,210 - +0.4 4,932,240 + -3.0 4,932,240 + +0.1 4,932,249 + -0.1 4,932,250 - +0.0 4,932,289 + +0.6 4,932,368 + AMB_RS22855 0.13 -1.2 4,932,369 - AMB_RS22855 0.13 +1.2 4,932,369 - AMB_RS22855 0.13 +0.0 4,932,369 - AMB_RS22855 0.13 +0.6 4,932,583 + AMB_RS22855 0.64 +0.0 4,932,583 + AMB_RS22855 0.64 -1.6 4,932,584 - AMB_RS22855 0.64 -0.0 4,932,842 + -0.7 4,932,842 + -1.8 4,932,842 + +0.0 4,932,843 - +0.3 4,932,843 - +0.1 4,932,845 - -0.7 4,932,850 + -0.7 4,932,850 + -1.2 4,932,850 + +0.2 4,932,850 + -0.3 4,932,851 - +0.2 4,932,862 + +0.2 4,932,863 - -0.6 4,932,863 - -0.7 4,932,863 - +0.4 4,932,908 - +0.6 4,932,908 - -1.0 4,932,952 + -0.7 4,932,952 + +0.3 4,932,952 + -0.0 4,932,953 - +0.1 4,932,955 + -2.0 4,933,007 + -0.4 4,933,050 + +0.3 4,933,051 - +0.7 4,933,051 - -0.7 4,933,059 + -0.2 4,933,060 - -1.2 4,933,346 + AMB_RS22860 0.31 -1.6 4,933,347 - AMB_RS22860 0.32 +0.2 4,933,439 + AMB_RS22860 0.43 -2.3 4,933,439 + AMB_RS22860 0.43 +0.1 4,933,439 + AMB_RS22860 0.43 -0.1 4,933,439 + AMB_RS22860 0.43 +0.0 4,933,439 + AMB_RS22860 0.43 +0.1 4,933,440 - AMB_RS22860 0.44 +0.0 4,933,440 - AMB_RS22860 0.44 -0.3 4,933,440 - AMB_RS22860 0.44 -1.2 4,933,457 + AMB_RS22860 0.46 -1.2 4,933,458 - AMB_RS22860 0.46 +0.9 4,933,458 - AMB_RS22860 0.46 -2.6 4,933,541 + AMB_RS22860 0.57 -0.3
Or see this region's nucleotide sequence