Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22495 and AMB_RS22500 are separated by 409 nucleotides AMB_RS22500 and AMB_RS22505 are separated by 89 nucleotides AMB_RS22505 and AMB_RS22510 are separated by 104 nucleotides
AMB_RS22495: AMB_RS22495 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 4,857,708 to 4,858,439
_RS22495
AMB_RS22500: AMB_RS22500 - ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent, at 4,858,849 to 4,861,194
_RS22500
AMB_RS22505: AMB_RS22505 - RNA-binding S4 domain-containing protein, at 4,861,284 to 4,861,565
_RS22505
AMB_RS22510: AMB_RS22510 - alanine--glyoxylate aminotransferase family protein, at 4,861,670 to 4,862,842
_RS22510
Position (kb)
4858
4859
4860
4861
4862 Strain fitness (log2 ratio)
-2
-1
0
1 at 4858.445 kb on + strand at 4858.445 kb on + strand at 4858.483 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.548 kb on - strand at 4858.563 kb on + strand at 4858.563 kb on + strand at 4858.566 kb on - strand at 4858.566 kb on - strand at 4858.672 kb on + strand at 4858.672 kb on + strand at 4858.673 kb on - strand at 4858.733 kb on + strand at 4858.818 kb on + strand at 4858.818 kb on + strand at 4858.894 kb on + strand at 4858.925 kb on + strand at 4859.504 kb on - strand, within AMB_RS22500 at 4859.504 kb on - strand, within AMB_RS22500 at 4859.554 kb on + strand, within AMB_RS22500 at 4859.554 kb on + strand, within AMB_RS22500 at 4859.554 kb on + strand, within AMB_RS22500 at 4859.554 kb on + strand, within AMB_RS22500 at 4859.555 kb on - strand, within AMB_RS22500 at 4859.555 kb on - strand, within AMB_RS22500 at 4859.560 kb on + strand, within AMB_RS22500 at 4859.560 kb on + strand, within AMB_RS22500 at 4859.561 kb on - strand, within AMB_RS22500 at 4859.561 kb on - strand, within AMB_RS22500 at 4859.561 kb on - strand, within AMB_RS22500 at 4859.561 kb on - strand, within AMB_RS22500 at 4859.618 kb on - strand, within AMB_RS22500 at 4859.818 kb on + strand, within AMB_RS22500 at 4859.819 kb on - strand, within AMB_RS22500 at 4860.043 kb on + strand, within AMB_RS22500 at 4860.043 kb on + strand, within AMB_RS22500 at 4860.043 kb on + strand, within AMB_RS22500 at 4860.044 kb on - strand, within AMB_RS22500 at 4860.044 kb on - strand, within AMB_RS22500 at 4860.208 kb on + strand, within AMB_RS22500 at 4860.208 kb on + strand, within AMB_RS22500 at 4860.209 kb on - strand, within AMB_RS22500 at 4860.209 kb on - strand, within AMB_RS22500 at 4860.211 kb on - strand, within AMB_RS22500 at 4860.265 kb on + strand, within AMB_RS22500 at 4860.319 kb on + strand, within AMB_RS22500 at 4860.386 kb on - strand, within AMB_RS22500 at 4860.458 kb on - strand, within AMB_RS22500 at 4860.686 kb on - strand, within AMB_RS22500 at 4860.686 kb on - strand, within AMB_RS22500 at 4860.686 kb on - strand, within AMB_RS22500 at 4860.721 kb on + strand, within AMB_RS22500 at 4860.721 kb on + strand, within AMB_RS22500 at 4860.721 kb on + strand, within AMB_RS22500 at 4860.721 kb on + strand, within AMB_RS22500 at 4860.722 kb on - strand, within AMB_RS22500 at 4860.722 kb on - strand, within AMB_RS22500 at 4860.931 kb on + strand, within AMB_RS22500 at 4860.931 kb on + strand, within AMB_RS22500 at 4861.048 kb on + strand at 4861.242 kb on + strand at 4861.242 kb on + strand at 4861.242 kb on + strand at 4861.242 kb on + strand at 4861.243 kb on - strand at 4861.459 kb on - strand, within AMB_RS22505 at 4861.564 kb on - strand at 4862.071 kb on - strand, within AMB_RS22510 at 4862.100 kb on + strand, within AMB_RS22510 at 4862.100 kb on + strand, within AMB_RS22510 at 4862.100 kb on + strand, within AMB_RS22510 at 4862.101 kb on - strand, within AMB_RS22510 at 4862.101 kb on - strand, within AMB_RS22510 at 4862.128 kb on - strand, within AMB_RS22510 at 4862.128 kb on - strand, within AMB_RS22510
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,858,445 + -0.1 4,858,445 + -0.7 4,858,483 - +0.0 4,858,548 - -2.5 4,858,548 - +1.6 4,858,548 - -0.7 4,858,548 - +0.2 4,858,548 - -1.0 4,858,563 + -1.0 4,858,563 + -1.6 4,858,566 - +0.0 4,858,566 - -0.1 4,858,672 + -0.7 4,858,672 + -1.2 4,858,673 - -0.4 4,858,733 + -1.2 4,858,818 + +0.2 4,858,818 + +1.0 4,858,894 + -2.2 4,858,925 + +0.6 4,859,504 - AMB_RS22500 0.28 -1.6 4,859,504 - AMB_RS22500 0.28 +0.1 4,859,554 + AMB_RS22500 0.30 +1.6 4,859,554 + AMB_RS22500 0.30 -0.4 4,859,554 + AMB_RS22500 0.30 -0.6 4,859,554 + AMB_RS22500 0.30 +0.1 4,859,555 - AMB_RS22500 0.30 -1.4 4,859,555 - AMB_RS22500 0.30 +1.6 4,859,560 + AMB_RS22500 0.30 -1.2 4,859,560 + AMB_RS22500 0.30 -0.6 4,859,561 - AMB_RS22500 0.30 +1.6 4,859,561 - AMB_RS22500 0.30 +0.0 4,859,561 - AMB_RS22500 0.30 +0.1 4,859,561 - AMB_RS22500 0.30 -0.0 4,859,618 - AMB_RS22500 0.33 -2.0 4,859,818 + AMB_RS22500 0.41 -0.2 4,859,819 - AMB_RS22500 0.41 +0.2 4,860,043 + AMB_RS22500 0.51 -2.0 4,860,043 + AMB_RS22500 0.51 +0.1 4,860,043 + AMB_RS22500 0.51 +0.0 4,860,044 - AMB_RS22500 0.51 +0.5 4,860,044 - AMB_RS22500 0.51 +0.3 4,860,208 + AMB_RS22500 0.58 +0.3 4,860,208 + AMB_RS22500 0.58 +0.0 4,860,209 - AMB_RS22500 0.58 +0.6 4,860,209 - AMB_RS22500 0.58 +0.1 4,860,211 - AMB_RS22500 0.58 +0.4 4,860,265 + AMB_RS22500 0.60 +0.3 4,860,319 + AMB_RS22500 0.63 -1.0 4,860,386 - AMB_RS22500 0.66 -1.4 4,860,458 - AMB_RS22500 0.69 +1.6 4,860,686 - AMB_RS22500 0.78 +0.2 4,860,686 - AMB_RS22500 0.78 -0.2 4,860,686 - AMB_RS22500 0.78 -1.2 4,860,721 + AMB_RS22500 0.80 -1.2 4,860,721 + AMB_RS22500 0.80 -1.2 4,860,721 + AMB_RS22500 0.80 -2.4 4,860,721 + AMB_RS22500 0.80 -0.4 4,860,722 - AMB_RS22500 0.80 +1.0 4,860,722 - AMB_RS22500 0.80 +0.0 4,860,931 + AMB_RS22500 0.89 +1.1 4,860,931 + AMB_RS22500 0.89 -0.2 4,861,048 + +1.6 4,861,242 + -0.2 4,861,242 + +0.3 4,861,242 + -2.1 4,861,242 + -0.7 4,861,243 - +0.6 4,861,459 - AMB_RS22505 0.62 +0.5 4,861,564 - -0.4 4,862,071 - AMB_RS22510 0.34 -0.7 4,862,100 + AMB_RS22510 0.37 -1.4 4,862,100 + AMB_RS22510 0.37 +0.5 4,862,100 + AMB_RS22510 0.37 +0.4 4,862,101 - AMB_RS22510 0.37 +0.1 4,862,101 - AMB_RS22510 0.37 -0.4 4,862,128 - AMB_RS22510 0.39 +0.4 4,862,128 - AMB_RS22510 0.39 +0.6
Or see this region's nucleotide sequence