Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22015 and AMB_RS22020 are separated by 299 nucleotides AMB_RS22020 and AMB_RS22025 are separated by 6 nucleotides AMB_RS22025 and AMB_RS22030 are separated by 138 nucleotides AMB_RS22030 and AMB_RS22035 are separated by 6 nucleotides
AMB_RS22015: AMB_RS22015 - methyltransferase domain-containing protein, at 4,756,303 to 4,757,073
_RS22015
AMB_RS22020: AMB_RS22020 - 50S ribosomal protein L34, at 4,757,373 to 4,757,507
_RS22020
AMB_RS22025: AMB_RS22025 - ribonuclease P protein component, at 4,757,514 to 4,757,915
_RS22025
AMB_RS22030: AMB_RS22030 - membrane protein insertion efficiency factor YidD, at 4,758,054 to 4,758,323
_RS22030
AMB_RS22035: AMB_RS22035 - membrane protein insertase YidC, at 4,758,330 to 4,760,069
_RS22035
Position (kb) 4758
4759 Strain fitness (log2 ratio) -1
0
1 at 4757.127 kb on + strand at 4757.128 kb on - strand at 4757.144 kb on + strand at 4757.145 kb on - strand at 4757.145 kb on - strand at 4757.145 kb on - strand at 4757.278 kb on + strand at 4757.965 kb on + strand at 4757.965 kb on + strand at 4758.255 kb on + strand, within AMB_RS22030 at 4758.255 kb on + strand, within AMB_RS22030 at 4758.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,757,127 + +1.0 4,757,128 - +0.1 4,757,144 + -1.7 4,757,145 - +0.9 4,757,145 - -0.7 4,757,145 - +0.5 4,757,278 + +1.6 4,757,965 + -1.4 4,757,965 + -0.1 4,758,255 + AMB_RS22030 0.74 +1.2 4,758,255 + AMB_RS22030 0.74 -0.2 4,758,321 + -1.2
Or see this region's nucleotide sequence