Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21865 and AMB_RS21870 are separated by 66 nucleotides AMB_RS21870 and AMB_RS21875 are separated by 134 nucleotides AMB_RS21875 and AMB_RS21880 are separated by 104 nucleotides
AMB_RS21865: AMB_RS21865 - J domain-containing protein, at 4,728,217 to 4,728,975
_RS21865
AMB_RS21870: AMB_RS21870 - DUF2189 domain-containing protein, at 4,729,042 to 4,729,833
_RS21870
AMB_RS21875: AMB_RS21875 - BAX inhibitor (BI)-1/YccA family protein, at 4,729,968 to 4,730,663
_RS21875
AMB_RS21880: AMB_RS21880 - glycosyl transferase family 1, at 4,730,768 to 4,733,289
_RS21880
Position (kb)
4729
4730
4731 Strain fitness (log2 ratio)
-2
-1
0
1 at 4728.973 kb on + strand at 4728.973 kb on + strand at 4728.974 kb on - strand at 4728.974 kb on - strand at 4728.974 kb on - strand at 4729.006 kb on + strand at 4729.006 kb on + strand at 4729.006 kb on + strand at 4729.006 kb on + strand at 4729.007 kb on - strand at 4729.007 kb on - strand at 4729.007 kb on - strand at 4729.007 kb on - strand at 4729.183 kb on + strand, within AMB_RS21870 at 4729.184 kb on - strand, within AMB_RS21870 at 4729.184 kb on - strand, within AMB_RS21870 at 4729.249 kb on + strand, within AMB_RS21870 at 4729.250 kb on - strand, within AMB_RS21870 at 4729.250 kb on - strand, within AMB_RS21870 at 4729.313 kb on - strand, within AMB_RS21870 at 4729.313 kb on - strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.417 kb on + strand, within AMB_RS21870 at 4729.418 kb on - strand, within AMB_RS21870 at 4729.418 kb on - strand, within AMB_RS21870 at 4729.418 kb on - strand, within AMB_RS21870 at 4729.418 kb on - strand, within AMB_RS21870 at 4729.839 kb on + strand at 4730.913 kb on + strand at 4730.914 kb on - strand at 4731.284 kb on - strand, within AMB_RS21880 at 4731.289 kb on + strand, within AMB_RS21880 at 4731.289 kb on + strand, within AMB_RS21880 at 4731.290 kb on - strand, within AMB_RS21880 at 4731.490 kb on - strand, within AMB_RS21880 at 4731.490 kb on - strand, within AMB_RS21880
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,728,973 + +0.0 4,728,973 + -0.4 4,728,974 - -0.4 4,728,974 - +0.7 4,728,974 - -0.7 4,729,006 + -0.1 4,729,006 + +0.4 4,729,006 + +0.8 4,729,006 + -0.0 4,729,007 - -2.6 4,729,007 - +1.0 4,729,007 - -1.0 4,729,007 - -0.0 4,729,183 + AMB_RS21870 0.18 +0.2 4,729,184 - AMB_RS21870 0.18 +0.1 4,729,184 - AMB_RS21870 0.18 +0.6 4,729,249 + AMB_RS21870 0.26 -1.1 4,729,250 - AMB_RS21870 0.26 +0.5 4,729,250 - AMB_RS21870 0.26 -0.7 4,729,313 - AMB_RS21870 0.34 -1.1 4,729,313 - AMB_RS21870 0.34 -0.1 4,729,417 + AMB_RS21870 0.47 +0.1 4,729,417 + AMB_RS21870 0.47 +0.0 4,729,417 + AMB_RS21870 0.47 +0.3 4,729,417 + AMB_RS21870 0.47 -0.1 4,729,417 + AMB_RS21870 0.47 -0.1 4,729,417 + AMB_RS21870 0.47 +0.6 4,729,418 - AMB_RS21870 0.47 -0.7 4,729,418 - AMB_RS21870 0.47 -2.1 4,729,418 - AMB_RS21870 0.47 -1.4 4,729,418 - AMB_RS21870 0.47 -0.1 4,729,839 + +0.8 4,730,913 + -1.7 4,730,914 - +0.6 4,731,284 - AMB_RS21880 0.20 +0.0 4,731,289 + AMB_RS21880 0.21 +0.4 4,731,289 + AMB_RS21880 0.21 -1.7 4,731,290 - AMB_RS21880 0.21 +0.1 4,731,490 - AMB_RS21880 0.29 +0.1 4,731,490 - AMB_RS21880 0.29 -0.7
Or see this region's nucleotide sequence