Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21105 and AMB_RS21110 overlap by 4 nucleotides AMB_RS21110 and AMB_RS21115 are separated by 72 nucleotides AMB_RS21115 and AMB_RS21120 are separated by 6 nucleotides AMB_RS21120 and AMB_RS21125 are separated by 59 nucleotides AMB_RS21125 and AMB_RS21130 overlap by 1 nucleotides AMB_RS21130 and AMB_RS21135 overlap by 28 nucleotides
AMB_RS21105: AMB_RS21105 - endonuclease III, at 4,585,822 to 4,586,511
_RS21105
AMB_RS21110: AMB_RS21110 - hemerythrin, at 4,586,508 to 4,586,924
_RS21110
AMB_RS21115: AMB_RS21115 - membrane-bound metal-dependent hydrolase, at 4,586,997 to 4,587,491
_RS21115
AMB_RS21120: AMB_RS21120 - DUF4337 domain-containing protein, at 4,587,498 to 4,588,103
_RS21120
AMB_RS21125: AMB_RS21125 - YciI family protein, at 4,588,163 to 4,588,438
_RS21125
AMB_RS21130: AMB_RS21130 - EVE domain-containing protein, at 4,588,438 to 4,588,851
_RS21130
AMB_RS21135: AMB_RS21135 - Rieske (2Fe-2S) protein, at 4,588,824 to 4,589,177
_RS21135
Position (kb) 4587
4588
4589 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4586.519 kb on - strand at 4586.533 kb on + strand at 4586.534 kb on - strand at 4586.534 kb on - strand at 4587.007 kb on + strand at 4587.008 kb on - strand at 4587.008 kb on - strand at 4587.008 kb on - strand at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.124 kb on + strand, within AMB_RS21115 at 4587.125 kb on - strand, within AMB_RS21115 at 4587.125 kb on - strand, within AMB_RS21115 at 4587.125 kb on - strand, within AMB_RS21115 at 4587.125 kb on - strand, within AMB_RS21115 at 4587.125 kb on - strand, within AMB_RS21115 at 4587.306 kb on + strand, within AMB_RS21115 at 4587.307 kb on - strand, within AMB_RS21115 at 4587.307 kb on - strand, within AMB_RS21115 at 4587.307 kb on - strand, within AMB_RS21115 at 4587.307 kb on - strand, within AMB_RS21115 at 4587.500 kb on - strand at 4587.500 kb on - strand at 4587.509 kb on + strand at 4587.510 kb on - strand at 4587.653 kb on - strand, within AMB_RS21120 at 4587.667 kb on + strand, within AMB_RS21120 at 4587.667 kb on + strand, within AMB_RS21120 at 4587.668 kb on - strand, within AMB_RS21120 at 4587.870 kb on - strand, within AMB_RS21120 at 4587.883 kb on + strand, within AMB_RS21120 at 4587.883 kb on + strand, within AMB_RS21120 at 4587.883 kb on + strand, within AMB_RS21120 at 4587.884 kb on - strand, within AMB_RS21120 at 4587.884 kb on - strand, within AMB_RS21120 at 4588.128 kb on - strand at 4588.155 kb on - strand at 4588.162 kb on + strand at 4588.162 kb on + strand at 4588.162 kb on + strand at 4588.163 kb on - strand at 4588.163 kb on - strand at 4588.377 kb on - strand, within AMB_RS21125 at 4588.377 kb on - strand, within AMB_RS21125 at 4588.377 kb on - strand, within AMB_RS21125 at 4588.419 kb on - strand at 4588.531 kb on + strand, within AMB_RS21130 at 4588.531 kb on + strand, within AMB_RS21130 at 4588.531 kb on + strand, within AMB_RS21130 at 4588.532 kb on - strand, within AMB_RS21130 at 4588.532 kb on - strand, within AMB_RS21130 at 4588.532 kb on - strand, within AMB_RS21130 at 4588.585 kb on + strand, within AMB_RS21130 at 4588.585 kb on + strand, within AMB_RS21130 at 4588.585 kb on + strand, within AMB_RS21130 at 4588.586 kb on - strand, within AMB_RS21130 at 4588.643 kb on - strand, within AMB_RS21130 at 4588.968 kb on + strand, within AMB_RS21135 at 4588.968 kb on + strand, within AMB_RS21135 at 4588.968 kb on + strand, within AMB_RS21135
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,586,519 - +0.5 4,586,533 + +0.5 4,586,534 - +0.9 4,586,534 - -0.1 4,587,007 + -0.2 4,587,008 - -0.8 4,587,008 - -1.9 4,587,008 - -2.3 4,587,124 + AMB_RS21115 0.26 +0.2 4,587,124 + AMB_RS21115 0.26 -0.2 4,587,124 + AMB_RS21115 0.26 -1.8 4,587,124 + AMB_RS21115 0.26 +0.6 4,587,124 + AMB_RS21115 0.26 -0.4 4,587,124 + AMB_RS21115 0.26 -0.5 4,587,124 + AMB_RS21115 0.26 -1.3 4,587,124 + AMB_RS21115 0.26 -1.5 4,587,125 - AMB_RS21115 0.26 +0.2 4,587,125 - AMB_RS21115 0.26 -3.4 4,587,125 - AMB_RS21115 0.26 -1.1 4,587,125 - AMB_RS21115 0.26 -0.4 4,587,125 - AMB_RS21115 0.26 -2.6 4,587,306 + AMB_RS21115 0.62 +0.3 4,587,307 - AMB_RS21115 0.63 +0.2 4,587,307 - AMB_RS21115 0.63 -1.6 4,587,307 - AMB_RS21115 0.63 +1.0 4,587,307 - AMB_RS21115 0.63 -0.8 4,587,500 - -0.8 4,587,500 - +0.1 4,587,509 + -0.3 4,587,510 - -0.2 4,587,653 - AMB_RS21120 0.26 -0.6 4,587,667 + AMB_RS21120 0.28 -0.4 4,587,667 + AMB_RS21120 0.28 -2.1 4,587,668 - AMB_RS21120 0.28 -4.1 4,587,870 - AMB_RS21120 0.61 -2.8 4,587,883 + AMB_RS21120 0.64 +0.1 4,587,883 + AMB_RS21120 0.64 -1.5 4,587,883 + AMB_RS21120 0.64 -2.1 4,587,884 - AMB_RS21120 0.64 +1.1 4,587,884 - AMB_RS21120 0.64 -2.5 4,588,128 - -1.5 4,588,155 - -0.8 4,588,162 + -1.5 4,588,162 + -1.6 4,588,162 + -0.6 4,588,163 - -0.8 4,588,163 - -0.7 4,588,377 - AMB_RS21125 0.78 -1.9 4,588,377 - AMB_RS21125 0.78 +0.1 4,588,377 - AMB_RS21125 0.78 -2.4 4,588,419 - +1.2 4,588,531 + AMB_RS21130 0.22 +0.5 4,588,531 + AMB_RS21130 0.22 +0.2 4,588,531 + AMB_RS21130 0.22 -0.1 4,588,532 - AMB_RS21130 0.23 -0.8 4,588,532 - AMB_RS21130 0.23 +0.5 4,588,532 - AMB_RS21130 0.23 -0.1 4,588,585 + AMB_RS21130 0.36 -1.1 4,588,585 + AMB_RS21130 0.36 +0.1 4,588,585 + AMB_RS21130 0.36 +0.1 4,588,586 - AMB_RS21130 0.36 +0.0 4,588,643 - AMB_RS21130 0.50 -1.1 4,588,968 + AMB_RS21135 0.41 +0.3 4,588,968 + AMB_RS21135 0.41 +1.3 4,588,968 + AMB_RS21135 0.41 +1.5
Or see this region's nucleotide sequence