Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20905 and AMB_RS20910 are separated by 230 nucleotides AMB_RS20910 and AMB_RS20915 overlap by 4 nucleotides AMB_RS20915 and AMB_RS20920 overlap by 4 nucleotides
AMB_RS20905: AMB_RS20905 - enoyl-CoA hydratase/carnithine racemase, at 4,540,357 to 4,541,121
_RS20905
AMB_RS20910: AMB_RS20910 - hypothetical protein, at 4,541,352 to 4,541,960
_RS20910
AMB_RS20915: AMB_RS20915 - hypothetical protein, at 4,541,957 to 4,542,541
_RS20915
AMB_RS20920: AMB_RS20920 - hemerythrin, at 4,542,538 to 4,542,960
_RS20920
Position (kb)
4541
4542 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4540.370 kb on + strand at 4540.370 kb on + strand at 4540.370 kb on + strand at 4540.370 kb on + strand at 4540.371 kb on - strand at 4540.371 kb on - strand at 4540.371 kb on - strand at 4540.371 kb on - strand at 4540.371 kb on - strand at 4540.450 kb on + strand, within AMB_RS20905 at 4540.450 kb on + strand, within AMB_RS20905 at 4540.450 kb on + strand, within AMB_RS20905 at 4540.451 kb on - strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.705 kb on + strand, within AMB_RS20905 at 4540.706 kb on - strand, within AMB_RS20905 at 4541.254 kb on + strand at 4541.255 kb on - strand at 4541.353 kb on + strand at 4541.353 kb on + strand at 4541.353 kb on + strand at 4541.354 kb on - strand at 4541.354 kb on - strand at 4541.354 kb on - strand at 4541.354 kb on - strand at 4541.354 kb on - strand at 4541.503 kb on + strand, within AMB_RS20910 at 4541.503 kb on + strand, within AMB_RS20910 at 4541.503 kb on + strand, within AMB_RS20910 at 4541.503 kb on + strand, within AMB_RS20910 at 4541.503 kb on + strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.504 kb on - strand, within AMB_RS20910 at 4541.506 kb on + strand, within AMB_RS20910 at 4541.645 kb on + strand, within AMB_RS20910 at 4541.645 kb on + strand, within AMB_RS20910 at 4541.812 kb on + strand, within AMB_RS20910 at 4541.812 kb on + strand, within AMB_RS20910 at 4541.812 kb on + strand, within AMB_RS20910 at 4541.812 kb on + strand, within AMB_RS20910 at 4541.812 kb on + strand, within AMB_RS20910 at 4541.813 kb on - strand, within AMB_RS20910 at 4541.813 kb on - strand, within AMB_RS20910 at 4541.813 kb on - strand, within AMB_RS20910 at 4542.081 kb on + strand, within AMB_RS20915 at 4542.081 kb on + strand, within AMB_RS20915 at 4542.082 kb on - strand, within AMB_RS20915 at 4542.097 kb on + strand, within AMB_RS20915 at 4542.097 kb on + strand, within AMB_RS20915 at 4542.097 kb on + strand, within AMB_RS20915 at 4542.162 kb on + strand, within AMB_RS20915 at 4542.163 kb on - strand, within AMB_RS20915 at 4542.297 kb on + strand, within AMB_RS20915 at 4542.298 kb on - strand, within AMB_RS20915 at 4542.360 kb on + strand, within AMB_RS20915 at 4542.360 kb on + strand, within AMB_RS20915 at 4542.360 kb on + strand, within AMB_RS20915 at 4542.360 kb on + strand, within AMB_RS20915 at 4542.361 kb on - strand, within AMB_RS20915 at 4542.777 kb on + strand, within AMB_RS20920 at 4542.806 kb on + strand, within AMB_RS20920 at 4542.807 kb on - strand, within AMB_RS20920 at 4542.943 kb on + strand at 4542.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,540,370 + +0.2 4,540,370 + +0.2 4,540,370 + +0.5 4,540,370 + +1.5 4,540,371 - +0.2 4,540,371 - -0.8 4,540,371 - -1.1 4,540,371 - -0.4 4,540,371 - -0.1 4,540,450 + AMB_RS20905 0.12 -1.8 4,540,450 + AMB_RS20905 0.12 +0.5 4,540,450 + AMB_RS20905 0.12 -0.5 4,540,451 - AMB_RS20905 0.12 +0.8 4,540,705 + AMB_RS20905 0.45 -0.8 4,540,705 + AMB_RS20905 0.45 +0.1 4,540,705 + AMB_RS20905 0.45 -0.2 4,540,705 + AMB_RS20905 0.45 -1.1 4,540,705 + AMB_RS20905 0.45 -0.5 4,540,705 + AMB_RS20905 0.45 -0.8 4,540,705 + AMB_RS20905 0.45 -0.0 4,540,706 - AMB_RS20905 0.46 +0.4 4,541,254 + -0.8 4,541,255 - -0.5 4,541,353 + +0.3 4,541,353 + +0.1 4,541,353 + -2.6 4,541,354 - -0.3 4,541,354 - -0.1 4,541,354 - -4.3 4,541,354 - +0.2 4,541,354 - -1.1 4,541,503 + AMB_RS20910 0.25 -0.5 4,541,503 + AMB_RS20910 0.25 -1.8 4,541,503 + AMB_RS20910 0.25 -1.5 4,541,503 + AMB_RS20910 0.25 +0.6 4,541,503 + AMB_RS20910 0.25 -2.1 4,541,504 - AMB_RS20910 0.25 +0.7 4,541,504 - AMB_RS20910 0.25 +0.2 4,541,504 - AMB_RS20910 0.25 -0.0 4,541,504 - AMB_RS20910 0.25 +0.0 4,541,504 - AMB_RS20910 0.25 -0.3 4,541,504 - AMB_RS20910 0.25 -0.1 4,541,506 + AMB_RS20910 0.25 +0.5 4,541,645 + AMB_RS20910 0.48 -0.2 4,541,645 + AMB_RS20910 0.48 -1.3 4,541,812 + AMB_RS20910 0.76 -0.3 4,541,812 + AMB_RS20910 0.76 +0.1 4,541,812 + AMB_RS20910 0.76 +0.8 4,541,812 + AMB_RS20910 0.76 -2.5 4,541,812 + AMB_RS20910 0.76 +0.3 4,541,813 - AMB_RS20910 0.76 +0.8 4,541,813 - AMB_RS20910 0.76 +1.0 4,541,813 - AMB_RS20910 0.76 -0.1 4,542,081 + AMB_RS20915 0.21 -0.1 4,542,081 + AMB_RS20915 0.21 -0.5 4,542,082 - AMB_RS20915 0.21 +0.1 4,542,097 + AMB_RS20915 0.24 -0.0 4,542,097 + AMB_RS20915 0.24 -0.1 4,542,097 + AMB_RS20915 0.24 +0.4 4,542,162 + AMB_RS20915 0.35 -1.3 4,542,163 - AMB_RS20915 0.35 -1.5 4,542,297 + AMB_RS20915 0.58 -0.1 4,542,298 - AMB_RS20915 0.58 -0.1 4,542,360 + AMB_RS20915 0.69 +0.5 4,542,360 + AMB_RS20915 0.69 -1.7 4,542,360 + AMB_RS20915 0.69 -0.3 4,542,360 + AMB_RS20915 0.69 -0.5 4,542,361 - AMB_RS20915 0.69 -2.1 4,542,777 + AMB_RS20920 0.57 -0.2 4,542,806 + AMB_RS20920 0.63 -1.9 4,542,807 - AMB_RS20920 0.64 -1.1 4,542,943 + +0.4 4,542,944 - +0.4
Or see this region's nucleotide sequence