Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20435 and AMB_RS20440 are separated by 29 nucleotides AMB_RS20440 and AMB_RS20445 are separated by 185 nucleotides
AMB_RS20435: AMB_RS20435 - DUF4102 domain-containing protein, at 4,443,415 to 4,444,560
_RS20435
AMB_RS20440: AMB_RS20440 - hypothetical protein, at 4,444,590 to 4,444,856
_RS20440
AMB_RS20445: AMB_RS20445 - resolvase, at 4,445,042 to 4,445,785
_RS20445
Position (kb)
4444
4445 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4443.801 kb on + strand, within AMB_RS20435 at 4443.801 kb on + strand, within AMB_RS20435 at 4443.848 kb on + strand, within AMB_RS20435 at 4443.928 kb on + strand, within AMB_RS20435 at 4443.928 kb on + strand, within AMB_RS20435 at 4443.929 kb on - strand, within AMB_RS20435 at 4444.025 kb on + strand, within AMB_RS20435 at 4444.111 kb on + strand, within AMB_RS20435 at 4444.111 kb on + strand, within AMB_RS20435 at 4444.112 kb on - strand, within AMB_RS20435 at 4444.112 kb on - strand, within AMB_RS20435 at 4444.112 kb on - strand, within AMB_RS20435 at 4444.136 kb on + strand, within AMB_RS20435 at 4444.137 kb on - strand, within AMB_RS20435 at 4444.137 kb on - strand, within AMB_RS20435 at 4444.137 kb on - strand, within AMB_RS20435 at 4444.137 kb on - strand, within AMB_RS20435 at 4444.167 kb on + strand, within AMB_RS20435 at 4444.168 kb on - strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.294 kb on + strand, within AMB_RS20435 at 4444.295 kb on - strand, within AMB_RS20435 at 4444.295 kb on - strand, within AMB_RS20435 at 4444.295 kb on - strand, within AMB_RS20435 at 4444.295 kb on - strand, within AMB_RS20435 at 4444.362 kb on + strand, within AMB_RS20435 at 4444.363 kb on - strand, within AMB_RS20435 at 4444.411 kb on + strand, within AMB_RS20435 at 4444.411 kb on + strand, within AMB_RS20435 at 4444.411 kb on + strand, within AMB_RS20435 at 4444.412 kb on - strand, within AMB_RS20435 at 4444.412 kb on - strand, within AMB_RS20435 at 4444.412 kb on - strand, within AMB_RS20435 at 4444.412 kb on - strand, within AMB_RS20435 at 4444.412 kb on - strand, within AMB_RS20435 at 4444.499 kb on - strand at 4444.577 kb on + strand at 4444.614 kb on - strand at 4444.659 kb on + strand, within AMB_RS20440 at 4444.664 kb on + strand, within AMB_RS20440 at 4444.665 kb on - strand, within AMB_RS20440 at 4444.684 kb on - strand, within AMB_RS20440 at 4444.684 kb on - strand, within AMB_RS20440 at 4444.684 kb on - strand, within AMB_RS20440 at 4444.696 kb on + strand, within AMB_RS20440 at 4444.697 kb on - strand, within AMB_RS20440 at 4444.883 kb on + strand at 4444.883 kb on + strand at 4444.883 kb on + strand at 4444.948 kb on + strand at 4444.948 kb on + strand at 4445.120 kb on - strand, within AMB_RS20445 at 4445.141 kb on + strand, within AMB_RS20445 at 4445.141 kb on + strand, within AMB_RS20445 at 4445.142 kb on - strand, within AMB_RS20445 at 4445.416 kb on - strand, within AMB_RS20445 at 4445.564 kb on + strand, within AMB_RS20445 at 4445.564 kb on + strand, within AMB_RS20445 at 4445.794 kb on + strand at 4445.794 kb on + strand at 4445.794 kb on + strand at 4445.794 kb on + strand at 4445.794 kb on + strand at 4445.794 kb on + strand at 4445.795 kb on - strand at 4445.795 kb on - strand at 4445.795 kb on - strand at 4445.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,443,801 + AMB_RS20435 0.34 -0.2 4,443,801 + AMB_RS20435 0.34 +0.5 4,443,848 + AMB_RS20435 0.38 +0.1 4,443,928 + AMB_RS20435 0.45 -1.0 4,443,928 + AMB_RS20435 0.45 +0.1 4,443,929 - AMB_RS20435 0.45 -0.5 4,444,025 + AMB_RS20435 0.53 +0.9 4,444,111 + AMB_RS20435 0.61 +0.4 4,444,111 + AMB_RS20435 0.61 +0.0 4,444,112 - AMB_RS20435 0.61 -1.1 4,444,112 - AMB_RS20435 0.61 +0.2 4,444,112 - AMB_RS20435 0.61 +0.1 4,444,136 + AMB_RS20435 0.63 -2.6 4,444,137 - AMB_RS20435 0.63 +0.5 4,444,137 - AMB_RS20435 0.63 -0.4 4,444,137 - AMB_RS20435 0.63 +0.3 4,444,137 - AMB_RS20435 0.63 +0.2 4,444,167 + AMB_RS20435 0.66 +0.3 4,444,168 - AMB_RS20435 0.66 +1.2 4,444,294 + AMB_RS20435 0.77 -0.2 4,444,294 + AMB_RS20435 0.77 -1.1 4,444,294 + AMB_RS20435 0.77 -1.9 4,444,294 + AMB_RS20435 0.77 -0.0 4,444,294 + AMB_RS20435 0.77 -1.1 4,444,294 + AMB_RS20435 0.77 -0.1 4,444,295 - AMB_RS20435 0.77 +0.5 4,444,295 - AMB_RS20435 0.77 -0.7 4,444,295 - AMB_RS20435 0.77 -0.1 4,444,295 - AMB_RS20435 0.77 +0.0 4,444,362 + AMB_RS20435 0.83 -1.3 4,444,363 - AMB_RS20435 0.83 -0.5 4,444,411 + AMB_RS20435 0.87 +0.5 4,444,411 + AMB_RS20435 0.87 -2.2 4,444,411 + AMB_RS20435 0.87 -0.4 4,444,412 - AMB_RS20435 0.87 -1.5 4,444,412 - AMB_RS20435 0.87 +0.3 4,444,412 - AMB_RS20435 0.87 -1.9 4,444,412 - AMB_RS20435 0.87 -0.1 4,444,412 - AMB_RS20435 0.87 -0.8 4,444,499 - +1.1 4,444,577 + -0.5 4,444,614 - +0.5 4,444,659 + AMB_RS20440 0.26 -1.3 4,444,664 + AMB_RS20440 0.28 -0.1 4,444,665 - AMB_RS20440 0.28 -0.5 4,444,684 - AMB_RS20440 0.35 +0.5 4,444,684 - AMB_RS20440 0.35 -1.3 4,444,684 - AMB_RS20440 0.35 -2.4 4,444,696 + AMB_RS20440 0.40 -1.7 4,444,697 - AMB_RS20440 0.40 +0.1 4,444,883 + -1.3 4,444,883 + +2.1 4,444,883 + -0.0 4,444,948 + -0.6 4,444,948 + +0.4 4,445,120 - AMB_RS20445 0.10 +0.2 4,445,141 + AMB_RS20445 0.13 +0.3 4,445,141 + AMB_RS20445 0.13 -0.5 4,445,142 - AMB_RS20445 0.13 -0.4 4,445,416 - AMB_RS20445 0.50 +1.5 4,445,564 + AMB_RS20445 0.70 -1.5 4,445,564 + AMB_RS20445 0.70 +0.3 4,445,794 + +1.5 4,445,794 + -0.5 4,445,794 + -0.8 4,445,794 + +0.5 4,445,794 + +0.0 4,445,794 + +0.1 4,445,795 - -0.5 4,445,795 - -1.1 4,445,795 - +0.7 4,445,795 - -2.2
Or see this region's nucleotide sequence