Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20315 and AMB_RS20320 overlap by 37 nucleotides AMB_RS20320 and AMB_RS20325 are separated by 202 nucleotides AMB_RS20325 and AMB_RS20330 are separated by 60 nucleotides
AMB_RS20315: AMB_RS20315 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 4,421,968 to 4,423,272
_RS20315
AMB_RS20320: AMB_RS20320 - hybrid sensor histidine kinase/response regulator, at 4,423,236 to 4,425,398
_RS20320
AMB_RS20325: AMB_RS20325 - flavodoxin family protein, at 4,425,601 to 4,426,164
_RS20325
AMB_RS20330: AMB_RS20330 - Rrf2 family transcriptional regulator, at 4,426,225 to 4,426,647
_RS20330
Position (kb)
4423
4424
4425
4426 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4423.338 kb on - strand at 4423.347 kb on + strand at 4423.347 kb on + strand at 4423.348 kb on - strand at 4423.564 kb on + strand, within AMB_RS20320 at 4423.565 kb on - strand, within AMB_RS20320 at 4423.565 kb on - strand, within AMB_RS20320 at 4423.965 kb on - strand, within AMB_RS20320 at 4424.857 kb on + strand, within AMB_RS20320 at 4424.858 kb on - strand, within AMB_RS20320 at 4424.964 kb on + strand, within AMB_RS20320 at 4424.964 kb on + strand, within AMB_RS20320 at 4424.964 kb on + strand, within AMB_RS20320 at 4424.964 kb on + strand, within AMB_RS20320 at 4424.964 kb on + strand, within AMB_RS20320 at 4424.965 kb on - strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.097 kb on + strand, within AMB_RS20320 at 4425.098 kb on - strand, within AMB_RS20320 at 4425.098 kb on - strand, within AMB_RS20320 at 4425.098 kb on - strand, within AMB_RS20320 at 4425.292 kb on + strand at 4425.292 kb on + strand at 4425.293 kb on - strand at 4425.410 kb on + strand at 4425.411 kb on - strand at 4425.411 kb on - strand at 4425.414 kb on + strand at 4425.415 kb on - strand at 4425.419 kb on + strand at 4425.420 kb on - strand at 4425.420 kb on - strand at 4425.436 kb on + strand at 4425.436 kb on + strand at 4425.436 kb on + strand at 4425.436 kb on + strand at 4425.437 kb on - strand at 4425.437 kb on - strand at 4425.602 kb on + strand at 4425.921 kb on - strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.980 kb on + strand, within AMB_RS20325 at 4425.981 kb on - strand, within AMB_RS20325 at 4425.981 kb on - strand, within AMB_RS20325 at 4425.981 kb on - strand, within AMB_RS20325 at 4425.981 kb on - strand, within AMB_RS20325 at 4426.122 kb on + strand at 4426.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,423,338 - -2.2 4,423,347 + +0.2 4,423,347 + +0.1 4,423,348 - -0.6 4,423,564 + AMB_RS20320 0.15 -0.3 4,423,565 - AMB_RS20320 0.15 -0.1 4,423,565 - AMB_RS20320 0.15 +0.1 4,423,965 - AMB_RS20320 0.34 +0.9 4,424,857 + AMB_RS20320 0.75 -1.1 4,424,858 - AMB_RS20320 0.75 +0.1 4,424,964 + AMB_RS20320 0.80 -1.2 4,424,964 + AMB_RS20320 0.80 -2.0 4,424,964 + AMB_RS20320 0.80 -0.7 4,424,964 + AMB_RS20320 0.80 -0.3 4,424,964 + AMB_RS20320 0.80 -0.5 4,424,965 - AMB_RS20320 0.80 -0.0 4,425,097 + AMB_RS20320 0.86 -0.2 4,425,097 + AMB_RS20320 0.86 -0.0 4,425,097 + AMB_RS20320 0.86 -2.4 4,425,097 + AMB_RS20320 0.86 +0.1 4,425,097 + AMB_RS20320 0.86 -1.5 4,425,097 + AMB_RS20320 0.86 -0.1 4,425,098 - AMB_RS20320 0.86 +0.5 4,425,098 - AMB_RS20320 0.86 -0.2 4,425,098 - AMB_RS20320 0.86 -0.8 4,425,292 + -3.1 4,425,292 + -0.5 4,425,293 - +0.2 4,425,410 + -1.3 4,425,411 - -1.5 4,425,411 - +0.9 4,425,414 + +0.1 4,425,415 - -1.8 4,425,419 + -1.1 4,425,420 - -0.8 4,425,420 - +0.5 4,425,436 + -0.3 4,425,436 + +0.1 4,425,436 + -0.2 4,425,436 + -0.2 4,425,437 - +0.5 4,425,437 - +0.0 4,425,602 + -1.8 4,425,921 - AMB_RS20325 0.57 +0.1 4,425,980 + AMB_RS20325 0.67 -2.4 4,425,980 + AMB_RS20325 0.67 -0.1 4,425,980 + AMB_RS20325 0.67 +0.5 4,425,980 + AMB_RS20325 0.67 -0.1 4,425,980 + AMB_RS20325 0.67 +0.3 4,425,980 + AMB_RS20325 0.67 +0.1 4,425,981 - AMB_RS20325 0.67 +0.3 4,425,981 - AMB_RS20325 0.67 -0.1 4,425,981 - AMB_RS20325 0.67 -0.5 4,425,981 - AMB_RS20325 0.67 -0.1 4,426,122 + +0.5 4,426,227 - -3.0
Or see this region's nucleotide sequence