Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20185 and AMB_RS20190 are separated by 113 nucleotides AMB_RS20190 and AMB_RS20195 overlap by 26 nucleotides AMB_RS20195 and AMB_RS20200 are separated by 170 nucleotides
AMB_RS20185: AMB_RS20185 - site-specific DNA-methyltransferase, at 4,398,261 to 4,399,337
_RS20185
AMB_RS20190: AMB_RS20190 - ribonuclease HII, at 4,399,451 to 4,400,056
_RS20190
AMB_RS20195: AMB_RS20195 - iron transporter MagA, at 4,400,031 to 4,401,335
_RS20195
AMB_RS20200: AMB_RS20200 - methyl-accepting chemotaxis protein, at 4,401,506 to 4,403,209
_RS20200
Position (kb)
4400
4401
4402 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4399.141 kb on + strand, within AMB_RS20185 at 4399.141 kb on + strand, within AMB_RS20185 at 4399.452 kb on + strand at 4399.462 kb on + strand at 4399.462 kb on + strand at 4399.462 kb on + strand at 4399.462 kb on + strand at 4399.462 kb on + strand at 4399.462 kb on + strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.463 kb on - strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.464 kb on + strand at 4399.465 kb on - strand at 4399.465 kb on - strand at 4399.465 kb on - strand at 4399.465 kb on - strand at 4399.465 kb on - strand at 4399.548 kb on + strand, within AMB_RS20190 at 4399.548 kb on + strand, within AMB_RS20190 at 4399.759 kb on - strand, within AMB_RS20190 at 4399.882 kb on + strand, within AMB_RS20190 at 4400.140 kb on + strand at 4400.140 kb on + strand at 4400.140 kb on + strand at 4400.140 kb on + strand at 4400.140 kb on + strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.141 kb on - strand at 4400.612 kb on - strand, within AMB_RS20195 at 4400.944 kb on - strand, within AMB_RS20195 at 4400.944 kb on - strand, within AMB_RS20195 at 4401.229 kb on + strand at 4401.229 kb on + strand at 4401.229 kb on + strand at 4401.307 kb on + strand at 4401.307 kb on + strand at 4401.308 kb on - strand at 4401.308 kb on - strand at 4401.347 kb on + strand at 4401.347 kb on + strand at 4401.347 kb on + strand at 4401.348 kb on - strand at 4401.348 kb on - strand at 4401.348 kb on - strand at 4401.371 kb on - strand at 4401.431 kb on - strand at 4401.472 kb on + strand at 4401.473 kb on - strand at 4401.473 kb on - strand at 4401.473 kb on - strand at 4401.594 kb on - strand at 4401.633 kb on - strand at 4401.633 kb on - strand at 4401.689 kb on + strand, within AMB_RS20200 at 4401.690 kb on - strand, within AMB_RS20200 at 4401.690 kb on - strand, within AMB_RS20200 at 4401.690 kb on - strand, within AMB_RS20200 at 4401.704 kb on + strand, within AMB_RS20200 at 4401.704 kb on + strand, within AMB_RS20200 at 4401.705 kb on - strand, within AMB_RS20200 at 4401.785 kb on + strand, within AMB_RS20200 at 4401.785 kb on + strand, within AMB_RS20200 at 4401.785 kb on + strand, within AMB_RS20200 at 4401.785 kb on + strand, within AMB_RS20200 at 4401.785 kb on + strand, within AMB_RS20200 at 4401.786 kb on - strand, within AMB_RS20200 at 4401.786 kb on - strand, within AMB_RS20200 at 4401.786 kb on - strand, within AMB_RS20200 at 4401.803 kb on + strand, within AMB_RS20200 at 4401.804 kb on - strand, within AMB_RS20200 at 4401.971 kb on + strand, within AMB_RS20200
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,399,141 + AMB_RS20185 0.82 +0.5 4,399,141 + AMB_RS20185 0.82 -0.1 4,399,452 + -0.1 4,399,462 + -0.5 4,399,462 + -0.5 4,399,462 + +0.3 4,399,462 + -0.1 4,399,462 + -2.1 4,399,462 + -1.1 4,399,463 - -1.7 4,399,463 - -0.4 4,399,463 - -0.8 4,399,463 - -0.5 4,399,463 - +0.2 4,399,463 - -0.1 4,399,463 - -0.0 4,399,463 - +0.1 4,399,464 + -0.1 4,399,464 + -0.1 4,399,464 + +0.3 4,399,464 + -0.2 4,399,464 + -1.3 4,399,464 + -0.3 4,399,464 + -0.1 4,399,465 - -0.4 4,399,465 - -0.9 4,399,465 - -1.5 4,399,465 - -0.1 4,399,465 - +0.2 4,399,548 + AMB_RS20190 0.16 -2.5 4,399,548 + AMB_RS20190 0.16 +1.5 4,399,759 - AMB_RS20190 0.51 -1.1 4,399,882 + AMB_RS20190 0.71 -0.2 4,400,140 + -0.5 4,400,140 + +0.0 4,400,140 + -0.5 4,400,140 + -0.3 4,400,140 + +0.5 4,400,141 - +0.0 4,400,141 - +0.5 4,400,141 - -1.3 4,400,141 - +0.5 4,400,141 - -0.4 4,400,141 - -1.3 4,400,141 - +0.4 4,400,141 - -0.8 4,400,612 - AMB_RS20195 0.45 -0.1 4,400,944 - AMB_RS20195 0.70 -1.6 4,400,944 - AMB_RS20195 0.70 -0.5 4,401,229 + +0.1 4,401,229 + +0.2 4,401,229 + -0.9 4,401,307 + +0.2 4,401,307 + -0.3 4,401,308 - +0.7 4,401,308 - -0.3 4,401,347 + -0.9 4,401,347 + -1.3 4,401,347 + -0.2 4,401,348 - +1.5 4,401,348 - -1.1 4,401,348 - -0.9 4,401,371 - -2.1 4,401,431 - -0.9 4,401,472 + +0.5 4,401,473 - +0.5 4,401,473 - +0.3 4,401,473 - -0.2 4,401,594 - -0.5 4,401,633 - +0.4 4,401,633 - +0.3 4,401,689 + AMB_RS20200 0.11 +0.3 4,401,690 - AMB_RS20200 0.11 +0.2 4,401,690 - AMB_RS20200 0.11 -0.1 4,401,690 - AMB_RS20200 0.11 +0.5 4,401,704 + AMB_RS20200 0.12 +0.2 4,401,704 + AMB_RS20200 0.12 -0.9 4,401,705 - AMB_RS20200 0.12 -0.1 4,401,785 + AMB_RS20200 0.16 -3.9 4,401,785 + AMB_RS20200 0.16 -1.7 4,401,785 + AMB_RS20200 0.16 -2.4 4,401,785 + AMB_RS20200 0.16 -0.4 4,401,785 + AMB_RS20200 0.16 -1.5 4,401,786 - AMB_RS20200 0.16 -0.3 4,401,786 - AMB_RS20200 0.16 +0.8 4,401,786 - AMB_RS20200 0.16 -0.8 4,401,803 + AMB_RS20200 0.17 -0.5 4,401,804 - AMB_RS20200 0.17 -0.9 4,401,971 + AMB_RS20200 0.27 +1.5
Or see this region's nucleotide sequence