Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20110 and AMB_RS20115 overlap by 4 nucleotides AMB_RS20115 and AMB_RS20120 are separated by 113 nucleotides AMB_RS20120 and AMB_RS20125 are separated by 146 nucleotides
AMB_RS20110: AMB_RS20110 - histidinol-phosphate aminotransferase, at 4,384,841 to 4,385,932
_RS20110
AMB_RS20115: AMB_RS20115 - prephenate dehydrogenase/arogenate dehydrogenase family protein, at 4,385,929 to 4,386,813
_RS20115
AMB_RS20120: AMB_RS20120 - VOC family protein, at 4,386,927 to 4,387,751
_RS20120
AMB_RS20125: AMB_RS20125 - CoA ester lyase, at 4,387,898 to 4,388,947
_RS20125
Position (kb)
4386
4387
4388 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4386.811 kb on + strand at 4386.823 kb on - strand at 4386.855 kb on + strand at 4386.959 kb on + strand at 4386.959 kb on + strand at 4386.960 kb on - strand at 4386.960 kb on - strand at 4386.960 kb on - strand at 4387.062 kb on + strand, within AMB_RS20120 at 4387.063 kb on - strand, within AMB_RS20120 at 4387.063 kb on - strand, within AMB_RS20120 at 4387.063 kb on - strand, within AMB_RS20120 at 4387.086 kb on + strand, within AMB_RS20120 at 4387.087 kb on - strand, within AMB_RS20120 at 4387.087 kb on - strand, within AMB_RS20120 at 4387.087 kb on - strand, within AMB_RS20120 at 4387.749 kb on + strand at 4387.749 kb on + strand at 4387.750 kb on - strand at 4387.750 kb on - strand at 4387.753 kb on + strand at 4387.753 kb on + strand at 4387.753 kb on + strand at 4387.753 kb on + strand at 4387.753 kb on + strand at 4387.754 kb on - strand at 4387.754 kb on - strand at 4387.892 kb on + strand at 4387.892 kb on + strand at 4387.892 kb on + strand at 4387.892 kb on + strand at 4387.899 kb on + strand at 4387.899 kb on + strand at 4387.900 kb on - strand at 4387.900 kb on - strand at 4387.900 kb on - strand at 4388.214 kb on + strand, within AMB_RS20125 at 4388.215 kb on - strand, within AMB_RS20125 at 4388.268 kb on + strand, within AMB_RS20125 at 4388.268 kb on + strand, within AMB_RS20125 at 4388.268 kb on + strand, within AMB_RS20125 at 4388.269 kb on - strand, within AMB_RS20125 at 4388.269 kb on - strand, within AMB_RS20125 at 4388.269 kb on - strand, within AMB_RS20125 at 4388.269 kb on - strand, within AMB_RS20125 at 4388.269 kb on - strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.337 kb on + strand, within AMB_RS20125 at 4388.338 kb on - strand, within AMB_RS20125 at 4388.482 kb on + strand, within AMB_RS20125 at 4388.550 kb on + strand, within AMB_RS20125 at 4388.550 kb on + strand, within AMB_RS20125 at 4388.551 kb on - strand, within AMB_RS20125 at 4388.551 kb on - strand, within AMB_RS20125
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,386,811 + +0.1 4,386,823 - -0.6 4,386,855 + +0.4 4,386,959 + +1.3 4,386,959 + -1.1 4,386,960 - -0.9 4,386,960 - -2.7 4,386,960 - -0.9 4,387,062 + AMB_RS20120 0.16 -0.9 4,387,063 - AMB_RS20120 0.16 -0.1 4,387,063 - AMB_RS20120 0.16 -0.5 4,387,063 - AMB_RS20120 0.16 -0.1 4,387,086 + AMB_RS20120 0.19 -0.8 4,387,087 - AMB_RS20120 0.19 -1.3 4,387,087 - AMB_RS20120 0.19 -0.2 4,387,087 - AMB_RS20120 0.19 -0.9 4,387,749 + +1.5 4,387,749 + -0.3 4,387,750 - -0.1 4,387,750 - +0.2 4,387,753 + -0.1 4,387,753 + -1.9 4,387,753 + -0.9 4,387,753 + +0.4 4,387,753 + -0.1 4,387,754 - -0.1 4,387,754 - -0.1 4,387,892 + -2.8 4,387,892 + -2.1 4,387,892 + +0.3 4,387,892 + +0.5 4,387,899 + +0.1 4,387,899 + -0.9 4,387,900 - -3.5 4,387,900 - -3.1 4,387,900 - +0.3 4,388,214 + AMB_RS20125 0.30 -1.3 4,388,215 - AMB_RS20125 0.30 +0.2 4,388,268 + AMB_RS20125 0.35 +0.8 4,388,268 + AMB_RS20125 0.35 -1.1 4,388,268 + AMB_RS20125 0.35 -1.1 4,388,269 - AMB_RS20125 0.35 -0.1 4,388,269 - AMB_RS20125 0.35 -2.9 4,388,269 - AMB_RS20125 0.35 -0.9 4,388,269 - AMB_RS20125 0.35 +0.3 4,388,269 - AMB_RS20125 0.35 -2.1 4,388,337 + AMB_RS20125 0.42 -2.2 4,388,337 + AMB_RS20125 0.42 +0.5 4,388,337 + AMB_RS20125 0.42 -1.3 4,388,337 + AMB_RS20125 0.42 +1.5 4,388,337 + AMB_RS20125 0.42 -2.1 4,388,337 + AMB_RS20125 0.42 -2.1 4,388,337 + AMB_RS20125 0.42 -0.1 4,388,337 + AMB_RS20125 0.42 -0.5 4,388,337 + AMB_RS20125 0.42 -2.0 4,388,337 + AMB_RS20125 0.42 +0.3 4,388,338 - AMB_RS20125 0.42 -0.1 4,388,482 + AMB_RS20125 0.56 -0.1 4,388,550 + AMB_RS20125 0.62 -0.1 4,388,550 + AMB_RS20125 0.62 +0.1 4,388,551 - AMB_RS20125 0.62 -0.0 4,388,551 - AMB_RS20125 0.62 -0.3
Or see this region's nucleotide sequence