Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS19980 and AMB_RS19985 are separated by 132 nucleotides AMB_RS19985 and AMB_RS19990 are separated by 260 nucleotides AMB_RS19990 and AMB_RS19995 are separated by 14 nucleotides
AMB_RS19980: AMB_RS19980 - CoA ester lyase, at 4,357,015 to 4,357,893
_RS19980
AMB_RS19985: AMB_RS19985 - MaoC family dehydratase, at 4,358,026 to 4,359,102
_RS19985
AMB_RS19990: AMB_RS19990 - succinate dehydrogenase, cytochrome b556 subunit, at 4,359,363 to 4,359,740
_RS19990
AMB_RS19995: AMB_RS19995 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 4,359,755 to 4,360,135
_RS19995
Position (kb)
4358
4359
4360 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4357.051 kb on + strand at 4357.051 kb on + strand at 4357.052 kb on - strand at 4357.052 kb on - strand at 4357.199 kb on - strand, within AMB_RS19980 at 4357.567 kb on + strand, within AMB_RS19980 at 4357.567 kb on + strand, within AMB_RS19980 at 4357.594 kb on + strand, within AMB_RS19980 at 4357.594 kb on + strand, within AMB_RS19980 at 4357.594 kb on + strand, within AMB_RS19980 at 4357.595 kb on - strand, within AMB_RS19980 at 4357.595 kb on - strand, within AMB_RS19980 at 4357.595 kb on - strand, within AMB_RS19980 at 4357.595 kb on - strand, within AMB_RS19980 at 4357.628 kb on - strand, within AMB_RS19980 at 4357.628 kb on - strand, within AMB_RS19980 at 4357.891 kb on + strand at 4357.891 kb on + strand at 4357.891 kb on + strand at 4357.891 kb on + strand at 4357.891 kb on + strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4357.892 kb on - strand at 4358.128 kb on + strand at 4358.128 kb on + strand at 4358.129 kb on - strand at 4358.141 kb on - strand, within AMB_RS19985 at 4358.308 kb on + strand, within AMB_RS19985 at 4358.309 kb on - strand, within AMB_RS19985 at 4358.339 kb on - strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.416 kb on + strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.417 kb on - strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.464 kb on + strand, within AMB_RS19985 at 4358.465 kb on - strand, within AMB_RS19985 at 4358.465 kb on - strand, within AMB_RS19985 at 4358.465 kb on - strand, within AMB_RS19985 at 4358.465 kb on - strand, within AMB_RS19985 at 4358.635 kb on + strand, within AMB_RS19985 at 4358.635 kb on + strand, within AMB_RS19985 at 4358.694 kb on + strand, within AMB_RS19985 at 4358.747 kb on - strand, within AMB_RS19985 at 4358.785 kb on + strand, within AMB_RS19985 at 4358.785 kb on + strand, within AMB_RS19985 at 4358.786 kb on - strand, within AMB_RS19985 at 4358.786 kb on - strand, within AMB_RS19985 at 4358.786 kb on - strand, within AMB_RS19985 at 4358.786 kb on - strand, within AMB_RS19985 at 4359.073 kb on + strand at 4359.073 kb on + strand at 4359.074 kb on - strand at 4359.100 kb on + strand at 4359.100 kb on + strand at 4359.101 kb on - strand at 4359.292 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 4,357,051 + +0.5 4,357,051 + +0.3 4,357,052 - +0.5 4,357,052 - -1.1 4,357,199 - AMB_RS19980 0.21 -2.2 4,357,567 + AMB_RS19980 0.63 -0.4 4,357,567 + AMB_RS19980 0.63 -0.2 4,357,594 + AMB_RS19980 0.66 +0.5 4,357,594 + AMB_RS19980 0.66 -3.7 4,357,594 + AMB_RS19980 0.66 -0.1 4,357,595 - AMB_RS19980 0.66 +0.8 4,357,595 - AMB_RS19980 0.66 -0.0 4,357,595 - AMB_RS19980 0.66 -1.5 4,357,595 - AMB_RS19980 0.66 -0.9 4,357,628 - AMB_RS19980 0.70 -0.5 4,357,628 - AMB_RS19980 0.70 -0.1 4,357,891 + -0.3 4,357,891 + -1.5 4,357,891 + -0.6 4,357,891 + +0.1 4,357,891 + -0.1 4,357,892 - -0.9 4,357,892 - -0.1 4,357,892 - -1.3 4,357,892 - -0.5 4,357,892 - +0.1 4,357,892 - -0.9 4,357,892 - -0.1 4,357,892 - -0.5 4,357,892 - -0.9 4,358,128 + -0.9 4,358,128 + +1.5 4,358,129 - -0.1 4,358,141 - AMB_RS19985 0.11 -0.2 4,358,308 + AMB_RS19985 0.26 +0.2 4,358,309 - AMB_RS19985 0.26 -0.2 4,358,339 - AMB_RS19985 0.29 +0.5 4,358,416 + AMB_RS19985 0.36 +0.5 4,358,416 + AMB_RS19985 0.36 -0.1 4,358,416 + AMB_RS19985 0.36 +0.5 4,358,416 + AMB_RS19985 0.36 -0.3 4,358,416 + AMB_RS19985 0.36 +0.4 4,358,416 + AMB_RS19985 0.36 +0.5 4,358,416 + AMB_RS19985 0.36 +0.5 4,358,417 - AMB_RS19985 0.36 -0.9 4,358,417 - AMB_RS19985 0.36 +0.1 4,358,417 - AMB_RS19985 0.36 -2.8 4,358,417 - AMB_RS19985 0.36 -0.3 4,358,417 - AMB_RS19985 0.36 -0.5 4,358,417 - AMB_RS19985 0.36 -1.7 4,358,464 + AMB_RS19985 0.41 -1.5 4,358,464 + AMB_RS19985 0.41 +0.5 4,358,464 + AMB_RS19985 0.41 -1.5 4,358,464 + AMB_RS19985 0.41 -0.2 4,358,464 + AMB_RS19985 0.41 +0.5 4,358,464 + AMB_RS19985 0.41 +0.1 4,358,465 - AMB_RS19985 0.41 -2.6 4,358,465 - AMB_RS19985 0.41 -1.1 4,358,465 - AMB_RS19985 0.41 +0.1 4,358,465 - AMB_RS19985 0.41 -0.1 4,358,635 + AMB_RS19985 0.57 +0.1 4,358,635 + AMB_RS19985 0.57 -0.5 4,358,694 + AMB_RS19985 0.62 +0.2 4,358,747 - AMB_RS19985 0.67 -0.5 4,358,785 + AMB_RS19985 0.70 -0.1 4,358,785 + AMB_RS19985 0.70 +0.2 4,358,786 - AMB_RS19985 0.71 +0.2 4,358,786 - AMB_RS19985 0.71 +0.5 4,358,786 - AMB_RS19985 0.71 -0.1 4,358,786 - AMB_RS19985 0.71 -0.4 4,359,073 + -2.8 4,359,073 + -2.4 4,359,074 - +0.2 4,359,100 + -0.1 4,359,100 + -0.3 4,359,101 - -0.9 4,359,292 + -2.1
Or see this region's nucleotide sequence