Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17965 and AMB_RS17970 overlap by 4 nucleotides AMB_RS17970 and AMB_RS17975 overlap by 1 nucleotides AMB_RS17975 and AMB_RS17980 are separated by 10 nucleotides AMB_RS17980 and AMB_RS17985 are separated by 135 nucleotides
AMB_RS17965: AMB_RS17965 - energy transducer TonB, at 3,902,709 to 3,903,374
_RS17965
AMB_RS17970: AMB_RS17970 - MotA/TolQ/ExbB proton channel family protein, at 3,903,371 to 3,904,171
_RS17970
AMB_RS17975: AMB_RS17975 - biopolymer transporter ExbD, at 3,904,171 to 3,904,605
_RS17975
AMB_RS17980: AMB_RS17980 - biopolymer transporter ExbD, at 3,904,616 to 3,905,032
_RS17980
AMB_RS17985: AMB_RS17985 - sulfite exporter TauE/SafE family protein, at 3,905,168 to 3,906,073
_RS17985
Position (kb)
3904
3905 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3903.354 kb on + strand at 3903.354 kb on + strand at 3903.355 kb on - strand at 3903.587 kb on + strand, within AMB_RS17970 at 3903.587 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.923 kb on + strand, within AMB_RS17970 at 3903.924 kb on - strand, within AMB_RS17970 at 3903.924 kb on - strand, within AMB_RS17970 at 3903.924 kb on - strand, within AMB_RS17970 at 3903.924 kb on - strand, within AMB_RS17970 at 3903.924 kb on - strand, within AMB_RS17970 at 3904.049 kb on + strand, within AMB_RS17970 at 3904.050 kb on - strand, within AMB_RS17970 at 3904.050 kb on - strand, within AMB_RS17970 at 3904.050 kb on - strand, within AMB_RS17970 at 3904.312 kb on - strand, within AMB_RS17975 at 3904.519 kb on + strand, within AMB_RS17975 at 3904.519 kb on + strand, within AMB_RS17975 at 3904.519 kb on + strand, within AMB_RS17975 at 3904.519 kb on + strand, within AMB_RS17975 at 3904.520 kb on - strand, within AMB_RS17975 at 3904.943 kb on + strand, within AMB_RS17980 at 3904.943 kb on + strand, within AMB_RS17980 at 3904.944 kb on - strand, within AMB_RS17980 at 3904.944 kb on - strand, within AMB_RS17980 at 3904.944 kb on - strand, within AMB_RS17980 at 3904.944 kb on - strand, within AMB_RS17980 at 3904.944 kb on - strand, within AMB_RS17980 at 3905.030 kb on + strand at 3905.031 kb on - strand at 3905.460 kb on + strand, within AMB_RS17985 at 3905.460 kb on + strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.461 kb on - strand, within AMB_RS17985 at 3905.516 kb on + strand, within AMB_RS17985 at 3905.516 kb on + strand, within AMB_RS17985 at 3905.517 kb on - strand, within AMB_RS17985 at 3905.560 kb on + strand, within AMB_RS17985 at 3905.561 kb on - strand, within AMB_RS17985 at 3905.561 kb on - strand, within AMB_RS17985 at 3905.561 kb on - strand, within AMB_RS17985 at 3905.561 kb on - strand, within AMB_RS17985 at 3905.562 kb on + strand, within AMB_RS17985 at 3905.562 kb on + strand, within AMB_RS17985 at 3905.562 kb on + strand, within AMB_RS17985 at 3905.563 kb on - strand, within AMB_RS17985 at 3905.563 kb on - strand, within AMB_RS17985 at 3905.563 kb on - strand, within AMB_RS17985 at 3905.563 kb on - strand, within AMB_RS17985
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,903,354 + +0.3 3,903,354 + -0.5 3,903,355 - +0.4 3,903,587 + AMB_RS17970 0.27 -0.1 3,903,587 + AMB_RS17970 0.27 +0.5 3,903,923 + AMB_RS17970 0.69 -1.1 3,903,923 + AMB_RS17970 0.69 +0.1 3,903,923 + AMB_RS17970 0.69 -0.6 3,903,923 + AMB_RS17970 0.69 -1.1 3,903,923 + AMB_RS17970 0.69 -0.0 3,903,923 + AMB_RS17970 0.69 -0.1 3,903,923 + AMB_RS17970 0.69 -0.6 3,903,923 + AMB_RS17970 0.69 +0.1 3,903,923 + AMB_RS17970 0.69 -1.1 3,903,923 + AMB_RS17970 0.69 -1.9 3,903,924 - AMB_RS17970 0.69 +1.4 3,903,924 - AMB_RS17970 0.69 -0.3 3,903,924 - AMB_RS17970 0.69 -1.9 3,903,924 - AMB_RS17970 0.69 -0.1 3,903,924 - AMB_RS17970 0.69 -1.9 3,904,049 + AMB_RS17970 0.85 -1.4 3,904,050 - AMB_RS17970 0.85 +0.5 3,904,050 - AMB_RS17970 0.85 -1.1 3,904,050 - AMB_RS17970 0.85 -0.1 3,904,312 - AMB_RS17975 0.32 -1.7 3,904,519 + AMB_RS17975 0.80 -0.9 3,904,519 + AMB_RS17975 0.80 +0.9 3,904,519 + AMB_RS17975 0.80 -1.0 3,904,519 + AMB_RS17975 0.80 -2.6 3,904,520 - AMB_RS17975 0.80 +1.4 3,904,943 + AMB_RS17980 0.78 -1.4 3,904,943 + AMB_RS17980 0.78 -0.3 3,904,944 - AMB_RS17980 0.79 -0.1 3,904,944 - AMB_RS17980 0.79 -0.6 3,904,944 - AMB_RS17980 0.79 -2.0 3,904,944 - AMB_RS17980 0.79 -2.9 3,904,944 - AMB_RS17980 0.79 -0.1 3,905,030 + -0.4 3,905,031 - -0.2 3,905,460 + AMB_RS17985 0.32 -2.3 3,905,460 + AMB_RS17985 0.32 -1.5 3,905,461 - AMB_RS17985 0.32 +0.3 3,905,461 - AMB_RS17985 0.32 -0.3 3,905,461 - AMB_RS17985 0.32 -0.1 3,905,461 - AMB_RS17985 0.32 +0.3 3,905,461 - AMB_RS17985 0.32 -0.7 3,905,461 - AMB_RS17985 0.32 -4.1 3,905,516 + AMB_RS17985 0.38 +0.1 3,905,516 + AMB_RS17985 0.38 +1.4 3,905,517 - AMB_RS17985 0.39 -0.2 3,905,560 + AMB_RS17985 0.43 -0.6 3,905,561 - AMB_RS17985 0.43 +1.4 3,905,561 - AMB_RS17985 0.43 -1.7 3,905,561 - AMB_RS17985 0.43 +0.7 3,905,561 - AMB_RS17985 0.43 +1.4 3,905,562 + AMB_RS17985 0.43 +0.1 3,905,562 + AMB_RS17985 0.43 +0.1 3,905,562 + AMB_RS17985 0.43 -1.6 3,905,563 - AMB_RS17985 0.44 -2.1 3,905,563 - AMB_RS17985 0.44 -0.6 3,905,563 - AMB_RS17985 0.44 +0.0 3,905,563 - AMB_RS17985 0.44 -0.5
Or see this region's nucleotide sequence