Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17545 and AMB_RS17550 are separated by 181 nucleotides AMB_RS17550 and AMB_RS17555 are separated by 410 nucleotides
AMB_RS17545: AMB_RS17545 - methyl-accepting chemotaxis protein, at 3,807,462 to 3,809,156
_RS17545
AMB_RS17550: AMB_RS17550 - 2-isopropylmalate synthase, at 3,809,338 to 3,810,999
_RS17550
AMB_RS17555: AMB_RS17555 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system, at 3,811,410 to 3,813,038
_RS17555
Position (kb)
3809
3810
3811 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3808.933 kb on + strand, within AMB_RS17545 at 3808.934 kb on - strand, within AMB_RS17545 at 3808.964 kb on - strand, within AMB_RS17545 at 3809.038 kb on + strand at 3809.038 kb on + strand at 3809.038 kb on + strand at 3809.038 kb on + strand at 3809.038 kb on + strand at 3809.039 kb on - strand at 3809.039 kb on - strand at 3809.285 kb on - strand at 3809.296 kb on - strand at 3809.296 kb on - strand at 3809.296 kb on - strand at 3809.333 kb on + strand at 3809.450 kb on + strand at 3809.450 kb on + strand at 3809.450 kb on + strand at 3809.450 kb on + strand at 3809.451 kb on - strand at 3809.451 kb on - strand at 3809.451 kb on - strand at 3809.499 kb on - strand at 3809.661 kb on - strand, within AMB_RS17550 at 3809.693 kb on + strand, within AMB_RS17550 at 3809.693 kb on + strand, within AMB_RS17550 at 3809.693 kb on + strand, within AMB_RS17550 at 3809.693 kb on + strand, within AMB_RS17550 at 3809.882 kb on + strand, within AMB_RS17550 at 3809.883 kb on - strand, within AMB_RS17550 at 3809.918 kb on + strand, within AMB_RS17550 at 3809.918 kb on + strand, within AMB_RS17550 at 3809.919 kb on - strand, within AMB_RS17550 at 3809.919 kb on - strand, within AMB_RS17550 at 3810.007 kb on - strand, within AMB_RS17550 at 3810.008 kb on + strand, within AMB_RS17550 at 3810.009 kb on - strand, within AMB_RS17550 at 3810.009 kb on - strand, within AMB_RS17550 at 3810.009 kb on - strand, within AMB_RS17550 at 3810.009 kb on - strand, within AMB_RS17550 at 3810.127 kb on - strand, within AMB_RS17550 at 3810.128 kb on + strand, within AMB_RS17550 at 3810.128 kb on + strand, within AMB_RS17550 at 3810.128 kb on + strand, within AMB_RS17550 at 3810.128 kb on + strand, within AMB_RS17550 at 3810.129 kb on - strand, within AMB_RS17550 at 3810.129 kb on - strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.617 kb on + strand, within AMB_RS17550 at 3810.618 kb on - strand, within AMB_RS17550 at 3810.618 kb on - strand, within AMB_RS17550 at 3810.618 kb on - strand, within AMB_RS17550 at 3810.618 kb on - strand, within AMB_RS17550 at 3810.618 kb on - strand, within AMB_RS17550 at 3810.723 kb on + strand, within AMB_RS17550 at 3810.724 kb on - strand, within AMB_RS17550 at 3810.975 kb on - strand at 3811.014 kb on + strand at 3811.014 kb on + strand at 3811.015 kb on - strand at 3811.243 kb on + strand at 3811.365 kb on - strand at 3811.379 kb on - strand at 3811.379 kb on - strand at 3811.386 kb on - strand at 3811.539 kb on + strand at 3811.539 kb on + strand at 3811.539 kb on + strand at 3811.539 kb on + strand at 3811.540 kb on - strand at 3811.540 kb on - strand at 3811.540 kb on - strand at 3811.540 kb on - strand at 3811.540 kb on - strand at 3811.717 kb on - strand, within AMB_RS17555 at 3811.719 kb on + strand, within AMB_RS17555 at 3811.720 kb on - strand, within AMB_RS17555 at 3811.745 kb on + strand, within AMB_RS17555 at 3811.745 kb on + strand, within AMB_RS17555 at 3811.745 kb on + strand, within AMB_RS17555 at 3811.746 kb on - strand, within AMB_RS17555
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,808,933 + AMB_RS17545 0.87 -1.9 3,808,934 - AMB_RS17545 0.87 -0.9 3,808,964 - AMB_RS17545 0.89 +0.4 3,809,038 + -0.2 3,809,038 + -0.5 3,809,038 + -0.0 3,809,038 + +0.2 3,809,038 + +0.5 3,809,039 - -0.9 3,809,039 - -2.0 3,809,285 - -0.9 3,809,296 - +0.0 3,809,296 - +0.4 3,809,296 - -1.1 3,809,333 + -0.1 3,809,450 + -0.4 3,809,450 + -0.1 3,809,450 + -0.9 3,809,450 + +0.2 3,809,451 - -1.9 3,809,451 - -2.1 3,809,451 - -0.7 3,809,499 - -1.9 3,809,661 - AMB_RS17550 0.19 -0.9 3,809,693 + AMB_RS17550 0.21 -2.2 3,809,693 + AMB_RS17550 0.21 -1.4 3,809,693 + AMB_RS17550 0.21 -0.6 3,809,693 + AMB_RS17550 0.21 -2.4 3,809,882 + AMB_RS17550 0.33 -1.2 3,809,883 - AMB_RS17550 0.33 -1.7 3,809,918 + AMB_RS17550 0.35 -2.4 3,809,918 + AMB_RS17550 0.35 +0.3 3,809,919 - AMB_RS17550 0.35 -2.5 3,809,919 - AMB_RS17550 0.35 -0.1 3,810,007 - AMB_RS17550 0.40 -4.3 3,810,008 + AMB_RS17550 0.40 -1.6 3,810,009 - AMB_RS17550 0.40 -2.1 3,810,009 - AMB_RS17550 0.40 -0.2 3,810,009 - AMB_RS17550 0.40 -0.6 3,810,009 - AMB_RS17550 0.40 -1.5 3,810,127 - AMB_RS17550 0.47 -1.1 3,810,128 + AMB_RS17550 0.48 -4.0 3,810,128 + AMB_RS17550 0.48 -2.1 3,810,128 + AMB_RS17550 0.48 -1.9 3,810,128 + AMB_RS17550 0.48 -1.7 3,810,129 - AMB_RS17550 0.48 -1.6 3,810,129 - AMB_RS17550 0.48 -1.2 3,810,617 + AMB_RS17550 0.77 -2.6 3,810,617 + AMB_RS17550 0.77 -1.3 3,810,617 + AMB_RS17550 0.77 -5.1 3,810,617 + AMB_RS17550 0.77 -1.1 3,810,617 + AMB_RS17550 0.77 -2.3 3,810,617 + AMB_RS17550 0.77 -1.1 3,810,617 + AMB_RS17550 0.77 -0.9 3,810,618 - AMB_RS17550 0.77 -1.0 3,810,618 - AMB_RS17550 0.77 -0.7 3,810,618 - AMB_RS17550 0.77 -2.0 3,810,618 - AMB_RS17550 0.77 -0.6 3,810,618 - AMB_RS17550 0.77 -2.5 3,810,723 + AMB_RS17550 0.83 +0.1 3,810,724 - AMB_RS17550 0.83 -0.6 3,810,975 - -2.7 3,811,014 + -1.8 3,811,014 + -0.4 3,811,015 - -1.4 3,811,243 + +0.4 3,811,365 - +0.3 3,811,379 - +0.2 3,811,379 - -0.8 3,811,386 - -1.7 3,811,539 + -0.1 3,811,539 + -1.7 3,811,539 + -0.9 3,811,539 + -2.3 3,811,540 - -0.4 3,811,540 - +0.6 3,811,540 - -0.9 3,811,540 - -0.9 3,811,540 - -1.6 3,811,717 - AMB_RS17555 0.19 -0.6 3,811,719 + AMB_RS17555 0.19 +0.4 3,811,720 - AMB_RS17555 0.19 -1.9 3,811,745 + AMB_RS17555 0.21 -0.4 3,811,745 + AMB_RS17555 0.21 -1.4 3,811,745 + AMB_RS17555 0.21 -0.3 3,811,746 - AMB_RS17555 0.21 +0.4
Or see this region's nucleotide sequence