Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17530 and AMB_RS17535 overlap by 4 nucleotides AMB_RS17535 and AMB_RS17540 are separated by 141 nucleotides AMB_RS17540 and AMB_RS17545 are separated by 46 nucleotides
AMB_RS17530: AMB_RS17530 - nif-specific transcriptional activator NifA, at 3,803,313 to 3,805,010
_RS17530
AMB_RS17535: AMB_RS17535 - protein-tyrosine-phosphatase, at 3,805,007 to 3,805,576
_RS17535
AMB_RS17540: AMB_RS17540 - potassium transporter KefB, at 3,805,718 to 3,807,415
_RS17540
AMB_RS17545: AMB_RS17545 - methyl-accepting chemotaxis protein, at 3,807,462 to 3,809,156
_RS17545
Position (kb)
3805
3806
3807
3808 Strain fitness (log2 ratio)
-2
-1
0
1 at 3805.145 kb on + strand, within AMB_RS17535 at 3805.145 kb on + strand, within AMB_RS17535 at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.632 kb on - strand at 3805.632 kb on - strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3806.055 kb on + strand, within AMB_RS17540 at 3807.144 kb on + strand, within AMB_RS17540 at 3807.144 kb on + strand, within AMB_RS17540 at 3807.265 kb on + strand at 3807.265 kb on + strand at 3807.266 kb on - strand at 3807.298 kb on - strand at 3807.429 kb on + strand at 3807.430 kb on - strand at 3807.430 kb on - strand at 3807.430 kb on - strand at 3807.430 kb on - strand at 3807.432 kb on - strand at 3807.432 kb on - strand at 3808.300 kb on + strand, within AMB_RS17545 at 3808.300 kb on + strand, within AMB_RS17545 at 3808.301 kb on - strand, within AMB_RS17545
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,805,145 + AMB_RS17535 0.24 -0.1 3,805,145 + AMB_RS17535 0.24 -1.4 3,805,631 + -0.9 3,805,631 + +0.4 3,805,631 + +0.3 3,805,631 + -0.1 3,805,631 + -2.1 3,805,631 + -1.1 3,805,632 - +0.0 3,805,632 - -0.9 3,805,638 + -1.1 3,805,638 + -0.5 3,805,638 + -0.3 3,805,638 + -0.7 3,805,639 - -1.1 3,805,639 - +0.3 3,805,639 - +0.1 3,805,639 - -0.1 3,805,639 - -1.4 3,805,639 - -0.6 3,806,055 + AMB_RS17540 0.20 -1.6 3,807,144 + AMB_RS17540 0.84 -1.9 3,807,144 + AMB_RS17540 0.84 -2.1 3,807,265 + +0.4 3,807,265 + +0.3 3,807,266 - +0.2 3,807,298 - +0.5 3,807,429 + +0.2 3,807,430 - +0.7 3,807,430 - -2.4 3,807,430 - -0.6 3,807,430 - -0.1 3,807,432 - -0.6 3,807,432 - -2.6 3,808,300 + AMB_RS17545 0.49 -1.2 3,808,300 + AMB_RS17545 0.49 -1.4 3,808,301 - AMB_RS17545 0.49 +0.0
Or see this region's nucleotide sequence