Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17520 and AMB_RS17525 are separated by 57 nucleotides AMB_RS17525 and AMB_RS17530 are separated by 158 nucleotides AMB_RS17530 and AMB_RS17535 overlap by 4 nucleotides AMB_RS17535 and AMB_RS17540 are separated by 141 nucleotides
AMB_RS17520: AMB_RS17520 - helix-turn-helix domain-containing protein, at 3,801,829 to 3,802,488
_RS17520
AMB_RS17525: AMB_RS17525 - hypothetical protein, at 3,802,546 to 3,803,154
_RS17525
AMB_RS17530: AMB_RS17530 - nif-specific transcriptional activator NifA, at 3,803,313 to 3,805,010
_RS17530
AMB_RS17535: AMB_RS17535 - protein-tyrosine-phosphatase, at 3,805,007 to 3,805,576
_RS17535
AMB_RS17540: AMB_RS17540 - potassium transporter KefB, at 3,805,718 to 3,807,415
_RS17540
Position (kb)
3803
3804
3805
3806 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3802.528 kb on + strand at 3802.528 kb on + strand at 3802.529 kb on - strand at 3802.529 kb on - strand at 3802.529 kb on - strand at 3802.529 kb on - strand at 3802.529 kb on - strand at 3802.756 kb on + strand, within AMB_RS17525 at 3802.757 kb on - strand, within AMB_RS17525 at 3802.757 kb on - strand, within AMB_RS17525 at 3802.757 kb on - strand, within AMB_RS17525 at 3803.098 kb on + strand at 3803.098 kb on + strand at 3803.099 kb on - strand at 3803.144 kb on + strand at 3803.145 kb on - strand at 3803.145 kb on - strand at 3803.163 kb on - strand at 3803.211 kb on + strand at 3803.211 kb on + strand at 3803.211 kb on + strand at 3803.218 kb on + strand at 3803.219 kb on - strand at 3803.226 kb on + strand at 3803.226 kb on + strand at 3803.226 kb on + strand at 3803.312 kb on + strand at 3803.313 kb on - strand at 3803.313 kb on - strand at 3803.362 kb on - strand at 3803.439 kb on + strand at 3803.440 kb on - strand at 3803.440 kb on - strand at 3803.550 kb on + strand, within AMB_RS17530 at 3803.638 kb on - strand, within AMB_RS17530 at 3803.638 kb on - strand, within AMB_RS17530 at 3803.638 kb on - strand, within AMB_RS17530 at 3803.763 kb on + strand, within AMB_RS17530 at 3803.764 kb on - strand, within AMB_RS17530 at 3803.764 kb on - strand, within AMB_RS17530 at 3803.764 kb on - strand, within AMB_RS17530 at 3804.414 kb on + strand, within AMB_RS17530 at 3804.414 kb on + strand, within AMB_RS17530 at 3804.414 kb on + strand, within AMB_RS17530 at 3804.415 kb on - strand, within AMB_RS17530 at 3804.415 kb on - strand, within AMB_RS17530 at 3804.415 kb on - strand, within AMB_RS17530 at 3804.492 kb on + strand, within AMB_RS17530 at 3804.492 kb on + strand, within AMB_RS17530 at 3804.493 kb on - strand, within AMB_RS17530 at 3804.493 kb on - strand, within AMB_RS17530 at 3804.493 kb on - strand, within AMB_RS17530 at 3804.493 kb on - strand, within AMB_RS17530 at 3804.493 kb on - strand, within AMB_RS17530 at 3804.574 kb on - strand, within AMB_RS17530 at 3805.145 kb on + strand, within AMB_RS17535 at 3805.145 kb on + strand, within AMB_RS17535 at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.631 kb on + strand at 3805.632 kb on - strand at 3805.632 kb on - strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.638 kb on + strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand at 3805.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,802,528 + +0.3 3,802,528 + +0.1 3,802,529 - -1.7 3,802,529 - +1.0 3,802,529 - +0.3 3,802,529 - -1.9 3,802,529 - +0.5 3,802,756 + AMB_RS17525 0.34 -0.9 3,802,757 - AMB_RS17525 0.35 +0.3 3,802,757 - AMB_RS17525 0.35 -1.1 3,802,757 - AMB_RS17525 0.35 -1.2 3,803,098 + -0.1 3,803,098 + -1.4 3,803,099 - -0.2 3,803,144 + -0.9 3,803,145 - -1.6 3,803,145 - +2.0 3,803,163 - +0.4 3,803,211 + +1.0 3,803,211 + -0.9 3,803,211 + -0.6 3,803,218 + -0.2 3,803,219 - -0.9 3,803,226 + +1.5 3,803,226 + +1.4 3,803,226 + +0.4 3,803,312 + -2.3 3,803,313 - -1.4 3,803,313 - +0.1 3,803,362 - -0.6 3,803,439 + -2.8 3,803,440 - -0.1 3,803,440 - +0.4 3,803,550 + AMB_RS17530 0.14 -2.1 3,803,638 - AMB_RS17530 0.19 -0.0 3,803,638 - AMB_RS17530 0.19 -1.1 3,803,638 - AMB_RS17530 0.19 +0.5 3,803,763 + AMB_RS17530 0.27 -0.1 3,803,764 - AMB_RS17530 0.27 -1.1 3,803,764 - AMB_RS17530 0.27 +0.4 3,803,764 - AMB_RS17530 0.27 -0.9 3,804,414 + AMB_RS17530 0.65 +0.0 3,804,414 + AMB_RS17530 0.65 -0.4 3,804,414 + AMB_RS17530 0.65 -2.6 3,804,415 - AMB_RS17530 0.65 -3.1 3,804,415 - AMB_RS17530 0.65 +0.6 3,804,415 - AMB_RS17530 0.65 -1.9 3,804,492 + AMB_RS17530 0.69 +0.2 3,804,492 + AMB_RS17530 0.69 -2.0 3,804,493 - AMB_RS17530 0.69 -1.4 3,804,493 - AMB_RS17530 0.69 +0.7 3,804,493 - AMB_RS17530 0.69 -3.3 3,804,493 - AMB_RS17530 0.69 -1.6 3,804,493 - AMB_RS17530 0.69 -0.9 3,804,574 - AMB_RS17530 0.74 -0.1 3,805,145 + AMB_RS17535 0.24 -0.1 3,805,145 + AMB_RS17535 0.24 -1.4 3,805,631 + -0.9 3,805,631 + +0.4 3,805,631 + +0.3 3,805,631 + -0.1 3,805,631 + -2.1 3,805,631 + -1.1 3,805,632 - +0.0 3,805,632 - -0.9 3,805,638 + -1.1 3,805,638 + -0.5 3,805,638 + -0.3 3,805,638 + -0.7 3,805,639 - -1.1 3,805,639 - +0.3 3,805,639 - +0.1 3,805,639 - -0.1 3,805,639 - -1.4 3,805,639 - -0.6
Or see this region's nucleotide sequence