Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17340 and AMB_RS17345 are separated by 45 nucleotides AMB_RS17345 and AMB_RS17350 are separated by 23 nucleotides AMB_RS17350 and AMB_RS17355 are separated by 99 nucleotides AMB_RS17355 and AMB_RS17360 are separated by 545 nucleotides
AMB_RS17340: AMB_RS17340 - rubredoxin, at 3,756,248 to 3,756,412
_RS17340
AMB_RS17345: AMB_RS17345 - rubredoxin, at 3,756,458 to 3,756,661
_RS17345
AMB_RS17350: AMB_RS17350 - rubrerythrin, at 3,756,685 to 3,757,161
_RS17350
AMB_RS17355: AMB_RS17355 - membrane protein, at 3,757,261 to 3,758,652
_RS17355
AMB_RS17360: AMB_RS17360 - site-specific recombinase, at 3,759,198 to 3,759,746
_RS17360
Position (kb)
3757
3758
3759 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3756.402 kb on + strand at 3756.402 kb on + strand at 3756.402 kb on + strand at 3756.615 kb on + strand, within AMB_RS17345 at 3756.615 kb on + strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.616 kb on - strand, within AMB_RS17345 at 3756.891 kb on - strand, within AMB_RS17350 at 3756.902 kb on + strand, within AMB_RS17350 at 3756.902 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.908 kb on + strand, within AMB_RS17350 at 3756.909 kb on - strand, within AMB_RS17350 at 3756.909 kb on - strand, within AMB_RS17350 at 3757.082 kb on + strand, within AMB_RS17350 at 3757.082 kb on + strand, within AMB_RS17350 at 3757.299 kb on - strand at 3757.299 kb on - strand at 3757.299 kb on - strand at 3757.299 kb on - strand at 3757.299 kb on - strand at 3757.934 kb on + strand, within AMB_RS17355 at 3757.935 kb on - strand, within AMB_RS17355 at 3758.133 kb on - strand, within AMB_RS17355 at 3758.585 kb on + strand at 3758.585 kb on + strand at 3758.585 kb on + strand at 3758.585 kb on + strand at 3758.585 kb on + strand at 3758.586 kb on - strand at 3758.586 kb on - strand at 3758.586 kb on - strand at 3758.690 kb on + strand at 3758.690 kb on + strand at 3758.690 kb on + strand at 3758.691 kb on - strand at 3758.691 kb on - strand at 3758.691 kb on - strand at 3758.851 kb on - strand at 3759.006 kb on + strand at 3759.052 kb on + strand at 3759.186 kb on + strand at 3759.306 kb on + strand, within AMB_RS17360
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,756,402 + -1.7 3,756,402 + -1.7 3,756,402 + -3.9 3,756,615 + AMB_RS17345 0.77 -2.8 3,756,615 + AMB_RS17345 0.77 -4.3 3,756,616 - AMB_RS17345 0.77 -2.8 3,756,616 - AMB_RS17345 0.77 +0.3 3,756,616 - AMB_RS17345 0.77 -0.4 3,756,891 - AMB_RS17350 0.43 -2.6 3,756,902 + AMB_RS17350 0.45 -5.9 3,756,902 + AMB_RS17350 0.45 -3.4 3,756,908 + AMB_RS17350 0.47 -1.8 3,756,908 + AMB_RS17350 0.47 -3.7 3,756,908 + AMB_RS17350 0.47 -1.9 3,756,909 - AMB_RS17350 0.47 -1.9 3,756,909 - AMB_RS17350 0.47 -2.2 3,757,082 + AMB_RS17350 0.83 -2.2 3,757,082 + AMB_RS17350 0.83 -2.3 3,757,299 - +0.4 3,757,299 - -1.2 3,757,299 - -0.2 3,757,299 - -2.6 3,757,299 - -0.3 3,757,934 + AMB_RS17355 0.48 -4.2 3,757,935 - AMB_RS17355 0.48 +0.5 3,758,133 - AMB_RS17355 0.63 -1.0 3,758,585 + -0.0 3,758,585 + -1.0 3,758,585 + -0.9 3,758,585 + -0.8 3,758,585 + +0.1 3,758,586 - -0.6 3,758,586 - +0.4 3,758,586 - -0.5 3,758,690 + +0.3 3,758,690 + -0.2 3,758,690 + -1.6 3,758,691 - +0.3 3,758,691 - +0.8 3,758,691 - +0.2 3,758,851 - -0.2 3,759,006 + +0.2 3,759,052 + -1.4 3,759,186 + -0.3 3,759,306 + AMB_RS17360 0.20 -0.9
Or see this region's nucleotide sequence