Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16895 and AMB_RS16900 are separated by 7 nucleotides AMB_RS16900 and AMB_RS16905 overlap by 1 nucleotides AMB_RS16905 and AMB_RS16910 overlap by 1 nucleotides AMB_RS16910 and AMB_RS16915 are separated by 146 nucleotides
AMB_RS16895: AMB_RS16895 - arsenate reductase ArsC, at 3,631,187 to 3,631,630
_RS16895
AMB_RS16900: AMB_RS16900 - UPF0262 family protein, at 3,631,638 to 3,632,111
_RS16900
AMB_RS16905: AMB_RS16905 - histidinol dehydrogenase, at 3,632,111 to 3,633,412
_RS16905
AMB_RS16910: AMB_RS16910 - ATP phosphoribosyltransferase, at 3,633,412 to 3,634,071
_RS16910
AMB_RS16915: AMB_RS16915 - DNA-binding response regulator, at 3,634,218 to 3,634,943
_RS16915
Position (kb)
3632
3633
3634 Strain fitness (log2 ratio)
-2
-1
0
1 at 3631.186 kb on - strand at 3631.189 kb on - strand at 3631.189 kb on - strand at 3631.521 kb on + strand, within AMB_RS16895 at 3631.521 kb on + strand, within AMB_RS16895 at 3631.522 kb on - strand, within AMB_RS16895 at 3631.522 kb on - strand, within AMB_RS16895 at 3631.522 kb on - strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.527 kb on + strand, within AMB_RS16895 at 3631.528 kb on - strand, within AMB_RS16895 at 3631.528 kb on - strand, within AMB_RS16895 at 3631.528 kb on - strand, within AMB_RS16895 at 3631.528 kb on - strand, within AMB_RS16895 at 3631.528 kb on - strand, within AMB_RS16895 at 3631.607 kb on + strand at 3631.607 kb on + strand at 3631.650 kb on - strand at 3631.820 kb on - strand, within AMB_RS16900 at 3631.840 kb on + strand, within AMB_RS16900 at 3631.841 kb on - strand, within AMB_RS16900 at 3631.841 kb on - strand, within AMB_RS16900 at 3631.949 kb on + strand, within AMB_RS16900 at 3631.949 kb on + strand, within AMB_RS16900 at 3631.949 kb on + strand, within AMB_RS16900 at 3631.949 kb on + strand, within AMB_RS16900 at 3631.950 kb on - strand, within AMB_RS16900 at 3631.950 kb on - strand, within AMB_RS16900 at 3631.951 kb on + strand, within AMB_RS16900 at 3631.951 kb on + strand, within AMB_RS16900 at 3631.951 kb on + strand, within AMB_RS16900 at 3631.952 kb on - strand, within AMB_RS16900 at 3631.952 kb on - strand, within AMB_RS16900 at 3631.952 kb on - strand, within AMB_RS16900 at 3631.952 kb on - strand, within AMB_RS16900 at 3631.952 kb on - strand, within AMB_RS16900 at 3631.961 kb on - strand, within AMB_RS16900 at 3631.993 kb on + strand, within AMB_RS16900 at 3631.993 kb on + strand, within AMB_RS16900 at 3631.993 kb on + strand, within AMB_RS16900 at 3631.994 kb on - strand, within AMB_RS16900 at 3631.994 kb on - strand, within AMB_RS16900 at 3631.994 kb on - strand, within AMB_RS16900 at 3632.014 kb on + strand, within AMB_RS16900 at 3632.015 kb on - strand, within AMB_RS16900 at 3632.065 kb on + strand at 3632.065 kb on + strand at 3632.066 kb on - strand at 3632.066 kb on - strand at 3632.066 kb on - strand at 3632.066 kb on - strand at 3632.066 kb on - strand at 3634.088 kb on - strand at 3634.146 kb on - strand at 3634.205 kb on + strand at 3634.205 kb on + strand at 3634.206 kb on - strand at 3634.208 kb on - strand at 3634.238 kb on + strand at 3634.238 kb on + strand at 3634.239 kb on - strand at 3634.239 kb on - strand at 3634.375 kb on + strand, within AMB_RS16915 at 3634.375 kb on + strand, within AMB_RS16915 at 3634.375 kb on + strand, within AMB_RS16915 at 3634.376 kb on - strand, within AMB_RS16915
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,631,186 - +0.9 3,631,189 - -0.4 3,631,189 - -0.9 3,631,521 + AMB_RS16895 0.75 +0.4 3,631,521 + AMB_RS16895 0.75 -0.1 3,631,522 - AMB_RS16895 0.75 -1.8 3,631,522 - AMB_RS16895 0.75 +0.1 3,631,522 - AMB_RS16895 0.75 +0.1 3,631,527 + AMB_RS16895 0.77 -0.3 3,631,527 + AMB_RS16895 0.77 -2.5 3,631,527 + AMB_RS16895 0.77 -0.6 3,631,527 + AMB_RS16895 0.77 -2.1 3,631,527 + AMB_RS16895 0.77 -1.2 3,631,527 + AMB_RS16895 0.77 -0.1 3,631,527 + AMB_RS16895 0.77 -0.6 3,631,527 + AMB_RS16895 0.77 -0.0 3,631,527 + AMB_RS16895 0.77 -1.4 3,631,527 + AMB_RS16895 0.77 +0.4 3,631,528 - AMB_RS16895 0.77 -0.5 3,631,528 - AMB_RS16895 0.77 +1.4 3,631,528 - AMB_RS16895 0.77 -0.6 3,631,528 - AMB_RS16895 0.77 -0.2 3,631,528 - AMB_RS16895 0.77 +0.6 3,631,607 + -0.1 3,631,607 + +0.2 3,631,650 - -0.5 3,631,820 - AMB_RS16900 0.38 -1.2 3,631,840 + AMB_RS16900 0.43 +0.7 3,631,841 - AMB_RS16900 0.43 -2.7 3,631,841 - AMB_RS16900 0.43 +0.0 3,631,949 + AMB_RS16900 0.66 +0.5 3,631,949 + AMB_RS16900 0.66 +0.2 3,631,949 + AMB_RS16900 0.66 -0.4 3,631,949 + AMB_RS16900 0.66 +0.4 3,631,950 - AMB_RS16900 0.66 +1.4 3,631,950 - AMB_RS16900 0.66 -2.2 3,631,951 + AMB_RS16900 0.66 -1.6 3,631,951 + AMB_RS16900 0.66 +1.5 3,631,951 + AMB_RS16900 0.66 +0.2 3,631,952 - AMB_RS16900 0.66 -0.4 3,631,952 - AMB_RS16900 0.66 +0.2 3,631,952 - AMB_RS16900 0.66 -1.8 3,631,952 - AMB_RS16900 0.66 -0.6 3,631,952 - AMB_RS16900 0.66 +0.8 3,631,961 - AMB_RS16900 0.68 -1.6 3,631,993 + AMB_RS16900 0.75 -0.3 3,631,993 + AMB_RS16900 0.75 +0.3 3,631,993 + AMB_RS16900 0.75 -1.8 3,631,994 - AMB_RS16900 0.75 +0.3 3,631,994 - AMB_RS16900 0.75 -0.1 3,631,994 - AMB_RS16900 0.75 -0.6 3,632,014 + AMB_RS16900 0.79 -1.9 3,632,015 - AMB_RS16900 0.80 -0.1 3,632,065 + +0.2 3,632,065 + -1.8 3,632,066 - -0.9 3,632,066 - -0.4 3,632,066 - -0.6 3,632,066 - +0.8 3,632,066 - -1.6 3,634,088 - -0.1 3,634,146 - -0.9 3,634,205 + -1.9 3,634,205 + -2.2 3,634,206 - -1.2 3,634,208 - +0.3 3,634,238 + +0.5 3,634,238 + +0.1 3,634,239 - +0.1 3,634,239 - -1.2 3,634,375 + AMB_RS16915 0.22 -0.4 3,634,375 + AMB_RS16915 0.22 +0.1 3,634,375 + AMB_RS16915 0.22 -1.8 3,634,376 - AMB_RS16915 0.22 -0.8
Or see this region's nucleotide sequence