Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16070 and AMB_RS16075 overlap by 8 nucleotides AMB_RS16075 and AMB_RS16080 are separated by 310 nucleotides AMB_RS16080 and AMB_RS16085 are separated by 20 nucleotides AMB_RS16085 and AMB_RS16090 are separated by 19 nucleotides AMB_RS16090 and AMB_RS16095 are separated by 27 nucleotides
AMB_RS16070: AMB_RS16070 - transcriptional regulator, at 3,448,711 to 3,449,076
_RS16070
AMB_RS16075: AMB_RS16075 - hypothetical protein, at 3,449,069 to 3,449,434
_RS16075
AMB_RS16080: AMB_RS16080 - tRNA-Glu, at 3,449,745 to 3,449,820
_RS16080
AMB_RS16085: AMB_RS16085 - Mov34/MPN/PAD-1, at 3,449,841 to 3,450,311
_RS16085
AMB_RS16090: AMB_RS16090 - hypothetical protein, at 3,450,331 to 3,450,660
_RS16090
AMB_RS16095: AMB_RS16095 - RluA family pseudouridine synthase, at 3,450,688 to 3,451,671
_RS16095
Position (kb)
3449
3450 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3448.902 kb on + strand, within AMB_RS16070 at 3448.902 kb on + strand, within AMB_RS16070 at 3448.902 kb on + strand, within AMB_RS16070 at 3448.903 kb on - strand, within AMB_RS16070 at 3448.903 kb on - strand, within AMB_RS16070 at 3449.218 kb on + strand, within AMB_RS16075 at 3449.218 kb on + strand, within AMB_RS16075 at 3449.218 kb on + strand, within AMB_RS16075 at 3449.362 kb on - strand, within AMB_RS16075 at 3449.362 kb on - strand, within AMB_RS16075 at 3449.374 kb on - strand, within AMB_RS16075 at 3449.453 kb on + strand at 3449.521 kb on - strand at 3449.590 kb on - strand at 3449.601 kb on - strand at 3449.645 kb on + strand at 3449.646 kb on - strand at 3449.800 kb on - strand, within AMB_RS16080 at 3449.800 kb on - strand, within AMB_RS16080 at 3449.800 kb on - strand, within AMB_RS16080 at 3449.807 kb on + strand, within AMB_RS16080 at 3449.808 kb on - strand, within AMB_RS16080 at 3449.842 kb on + strand at 3449.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,448,902 + AMB_RS16070 0.52 -1.4 3,448,902 + AMB_RS16070 0.52 +0.4 3,448,902 + AMB_RS16070 0.52 +0.0 3,448,903 - AMB_RS16070 0.52 -0.6 3,448,903 - AMB_RS16070 0.52 -1.8 3,449,218 + AMB_RS16075 0.41 -0.0 3,449,218 + AMB_RS16075 0.41 +0.1 3,449,218 + AMB_RS16075 0.41 +0.0 3,449,362 - AMB_RS16075 0.80 +0.3 3,449,362 - AMB_RS16075 0.80 -0.9 3,449,374 - AMB_RS16075 0.83 -1.9 3,449,453 + +0.1 3,449,521 - -2.9 3,449,590 - -1.6 3,449,601 - +0.2 3,449,645 + +0.1 3,449,646 - -2.4 3,449,800 - AMB_RS16080 0.72 -1.7 3,449,800 - AMB_RS16080 0.72 -2.1 3,449,800 - AMB_RS16080 0.72 -2.3 3,449,807 + AMB_RS16080 0.82 -0.4 3,449,808 - AMB_RS16080 0.83 -1.8 3,449,842 + -1.6 3,449,843 - -1.2
Or see this region's nucleotide sequence