Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14510 and AMB_RS14515 are separated by 69 nucleotides AMB_RS14515 and AMB_RS14520 overlap by 1 nucleotides AMB_RS14520 and AMB_RS14525 overlap by 40 nucleotides AMB_RS14525 and AMB_RS14530 overlap by 86 nucleotides
AMB_RS14510: AMB_RS14510 - (2Fe-2S)-binding protein, at 3,082,293 to 3,082,754
_RS14510
AMB_RS14515: AMB_RS14515 - DUF4065 domain-containing protein, at 3,082,824 to 3,083,393
_RS14515
AMB_RS14520: AMB_RS14520 - 1,4-beta-N-acetylmuramidase, at 3,083,393 to 3,084,229
_RS14520
AMB_RS14525: AMB_RS14525 - xylanase/chitin deacetylase, at 3,084,190 to 3,085,071
_RS14525
AMB_RS14530: AMB_RS14530 - acyl dehydratase, at 3,084,986 to 3,085,453
_RS14530
Position (kb)
3083
3084
3085 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3082.790 kb on + strand at 3082.790 kb on + strand at 3082.791 kb on - strand at 3082.832 kb on - strand at 3082.896 kb on + strand, within AMB_RS14515 at 3082.896 kb on + strand, within AMB_RS14515 at 3082.897 kb on - strand, within AMB_RS14515 at 3082.897 kb on - strand, within AMB_RS14515 at 3082.929 kb on + strand, within AMB_RS14515 at 3082.930 kb on - strand, within AMB_RS14515 at 3082.930 kb on - strand, within AMB_RS14515 at 3082.930 kb on - strand, within AMB_RS14515 at 3082.930 kb on - strand, within AMB_RS14515 at 3082.930 kb on - strand, within AMB_RS14515 at 3082.944 kb on + strand, within AMB_RS14515 at 3082.944 kb on + strand, within AMB_RS14515 at 3082.945 kb on - strand, within AMB_RS14515 at 3082.959 kb on + strand, within AMB_RS14515 at 3082.959 kb on + strand, within AMB_RS14515 at 3082.959 kb on + strand, within AMB_RS14515 at 3082.960 kb on - strand, within AMB_RS14515 at 3083.122 kb on - strand, within AMB_RS14515 at 3083.130 kb on + strand, within AMB_RS14515 at 3083.235 kb on + strand, within AMB_RS14515 at 3083.235 kb on + strand, within AMB_RS14515 at 3083.235 kb on + strand, within AMB_RS14515 at 3083.236 kb on - strand, within AMB_RS14515 at 3083.236 kb on - strand, within AMB_RS14515 at 3083.236 kb on - strand, within AMB_RS14515 at 3083.236 kb on - strand, within AMB_RS14515 at 3083.239 kb on - strand, within AMB_RS14515 at 3083.239 kb on - strand, within AMB_RS14515 at 3083.430 kb on + strand at 3083.900 kb on + strand, within AMB_RS14520 at 3083.901 kb on - strand, within AMB_RS14520 at 3083.901 kb on - strand, within AMB_RS14520 at 3083.901 kb on - strand, within AMB_RS14520 at 3083.901 kb on - strand, within AMB_RS14520 at 3084.080 kb on + strand, within AMB_RS14520 at 3084.080 kb on + strand, within AMB_RS14520 at 3084.192 kb on - strand at 3084.501 kb on - strand, within AMB_RS14525
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,082,790 + -0.3 3,082,790 + -1.9 3,082,791 - -0.2 3,082,832 - -0.2 3,082,896 + AMB_RS14515 0.13 -0.3 3,082,896 + AMB_RS14515 0.13 -3.4 3,082,897 - AMB_RS14515 0.13 -0.9 3,082,897 - AMB_RS14515 0.13 -0.0 3,082,929 + AMB_RS14515 0.18 -3.3 3,082,930 - AMB_RS14515 0.19 -0.2 3,082,930 - AMB_RS14515 0.19 -0.1 3,082,930 - AMB_RS14515 0.19 +0.5 3,082,930 - AMB_RS14515 0.19 -0.2 3,082,930 - AMB_RS14515 0.19 -1.8 3,082,944 + AMB_RS14515 0.21 +0.2 3,082,944 + AMB_RS14515 0.21 -2.3 3,082,945 - AMB_RS14515 0.21 -1.4 3,082,959 + AMB_RS14515 0.24 -1.2 3,082,959 + AMB_RS14515 0.24 -0.4 3,082,959 + AMB_RS14515 0.24 +0.1 3,082,960 - AMB_RS14515 0.24 -0.6 3,083,122 - AMB_RS14515 0.52 -0.4 3,083,130 + AMB_RS14515 0.54 -0.6 3,083,235 + AMB_RS14515 0.72 +0.0 3,083,235 + AMB_RS14515 0.72 -0.3 3,083,235 + AMB_RS14515 0.72 +0.1 3,083,236 - AMB_RS14515 0.72 -1.8 3,083,236 - AMB_RS14515 0.72 -0.2 3,083,236 - AMB_RS14515 0.72 -1.8 3,083,236 - AMB_RS14515 0.72 +1.4 3,083,239 - AMB_RS14515 0.73 -0.5 3,083,239 - AMB_RS14515 0.73 -0.9 3,083,430 + -0.9 3,083,900 + AMB_RS14520 0.61 -2.0 3,083,901 - AMB_RS14520 0.61 -2.0 3,083,901 - AMB_RS14520 0.61 -2.0 3,083,901 - AMB_RS14520 0.61 -2.1 3,083,901 - AMB_RS14520 0.61 -1.0 3,084,080 + AMB_RS14520 0.82 -1.7 3,084,080 + AMB_RS14520 0.82 -0.5 3,084,192 - -0.3 3,084,501 - AMB_RS14525 0.35 -0.0
Or see this region's nucleotide sequence