Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23475 and AMB_RS14285 are separated by 165 nucleotides AMB_RS14285 and AMB_RS14290 overlap by 4 nucleotides AMB_RS14290 and AMB_RS14295 overlap by 25 nucleotides
AMB_RS23475: AMB_RS23475 - YkgJ family cysteine cluster protein, at 3,035,685 to 3,036,416
_RS23475
AMB_RS14285: AMB_RS14285 - YaiI/YqxD family protein, at 3,036,582 to 3,037,037
_RS14285
AMB_RS14290: AMB_RS14290 - exopolyphosphatase, at 3,037,034 to 3,038,549
_RS14290
AMB_RS14295: AMB_RS14295 - RNA degradosome polyphosphate kinase, at 3,038,525 to 3,040,663
_RS14295
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3036.402 kb on - strand at 3036.402 kb on - strand at 3036.402 kb on - strand at 3036.492 kb on - strand at 3036.557 kb on + strand at 3036.558 kb on - strand at 3036.568 kb on - strand at 3036.967 kb on + strand, within AMB_RS14285 at 3037.025 kb on - strand at 3037.152 kb on + strand at 3037.152 kb on + strand at 3037.152 kb on + strand at 3037.152 kb on + strand at 3037.153 kb on - strand at 3037.153 kb on - strand at 3037.153 kb on - strand at 3037.153 kb on - strand at 3037.153 kb on - strand at 3037.213 kb on + strand, within AMB_RS14290 at 3037.213 kb on + strand, within AMB_RS14290 at 3037.221 kb on - strand, within AMB_RS14290 at 3037.653 kb on + strand, within AMB_RS14290 at 3037.654 kb on - strand, within AMB_RS14290 at 3037.654 kb on - strand, within AMB_RS14290 at 3037.654 kb on - strand, within AMB_RS14290 at 3037.654 kb on - strand, within AMB_RS14290 at 3037.654 kb on - strand, within AMB_RS14290 at 3037.914 kb on + strand, within AMB_RS14290 at 3037.914 kb on + strand, within AMB_RS14290 at 3037.915 kb on - strand, within AMB_RS14290 at 3038.100 kb on - strand, within AMB_RS14290 at 3038.445 kb on + strand at 3038.446 kb on - strand at 3038.446 kb on - strand at 3038.446 kb on - strand at 3038.446 kb on - strand at 3038.607 kb on + strand at 3038.607 kb on + strand at 3038.652 kb on + strand at 3038.652 kb on + strand at 3038.652 kb on + strand at 3039.123 kb on + strand, within AMB_RS14295 at 3039.123 kb on + strand, within AMB_RS14295 at 3039.123 kb on + strand, within AMB_RS14295 at 3039.123 kb on + strand, within AMB_RS14295 at 3039.124 kb on - strand, within AMB_RS14295 at 3039.124 kb on - strand, within AMB_RS14295 at 3039.246 kb on + strand, within AMB_RS14295 at 3039.247 kb on - strand, within AMB_RS14295 at 3039.270 kb on + strand, within AMB_RS14295 at 3039.294 kb on + strand, within AMB_RS14295 at 3039.294 kb on + strand, within AMB_RS14295
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 3,036,402 - -0.3 3,036,402 - +0.7 3,036,402 - -2.1 3,036,492 - +1.2 3,036,557 + -0.2 3,036,558 - +1.0 3,036,568 - +0.4 3,036,967 + AMB_RS14285 0.84 +1.3 3,037,025 - +0.2 3,037,152 + -0.1 3,037,152 + -0.2 3,037,152 + -1.2 3,037,152 + +0.1 3,037,153 - +0.8 3,037,153 - +0.3 3,037,153 - -0.5 3,037,153 - -2.1 3,037,153 - -2.9 3,037,213 + AMB_RS14290 0.12 +0.7 3,037,213 + AMB_RS14290 0.12 -1.6 3,037,221 - AMB_RS14290 0.12 +0.2 3,037,653 + AMB_RS14290 0.41 -1.6 3,037,654 - AMB_RS14290 0.41 -0.2 3,037,654 - AMB_RS14290 0.41 -0.1 3,037,654 - AMB_RS14290 0.41 -1.0 3,037,654 - AMB_RS14290 0.41 -1.6 3,037,654 - AMB_RS14290 0.41 -0.4 3,037,914 + AMB_RS14290 0.58 -0.1 3,037,914 + AMB_RS14290 0.58 -0.2 3,037,915 - AMB_RS14290 0.58 +0.6 3,038,100 - AMB_RS14290 0.70 -0.8 3,038,445 + -0.1 3,038,446 - -2.2 3,038,446 - -3.1 3,038,446 - +0.5 3,038,446 - -0.5 3,038,607 + -1.2 3,038,607 + +0.4 3,038,652 + +2.6 3,038,652 + +0.2 3,038,652 + -1.2 3,039,123 + AMB_RS14295 0.28 -1.1 3,039,123 + AMB_RS14295 0.28 +0.3 3,039,123 + AMB_RS14295 0.28 -0.9 3,039,123 + AMB_RS14295 0.28 -0.6 3,039,124 - AMB_RS14295 0.28 -1.2 3,039,124 - AMB_RS14295 0.28 -0.1 3,039,246 + AMB_RS14295 0.34 +0.5 3,039,247 - AMB_RS14295 0.34 -0.2 3,039,270 + AMB_RS14295 0.35 -0.4 3,039,294 + AMB_RS14295 0.36 -0.2 3,039,294 + AMB_RS14295 0.36 -1.2
Or see this region's nucleotide sequence