Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23465 and AMB_RS14025 are separated by 320 nucleotides AMB_RS14025 and AMB_RS14030 are separated by 104 nucleotides AMB_RS14030 and AMB_RS14035 are separated by 228 nucleotides
AMB_RS23465: AMB_RS23465 - methyl-accepting chemotaxis protein, at 2,974,025 to 2,976,097
_RS23465
AMB_RS14025: AMB_RS14025 - tRNA-Val, at 2,976,418 to 2,976,493
_RS14025
AMB_RS14030: AMB_RS14030 - HU family DNA-binding protein, at 2,976,598 to 2,976,870
_RS14030
AMB_RS14035: AMB_RS14035 - endopeptidase La, at 2,977,099 to 2,979,510
_RS14035
Position (kb)
2976
2977 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2975.521 kb on + strand, within AMB_RS23465 at 2975.521 kb on + strand, within AMB_RS23465 at 2975.577 kb on + strand, within AMB_RS23465 at 2975.577 kb on + strand, within AMB_RS23465 at 2975.578 kb on - strand, within AMB_RS23465 at 2975.578 kb on - strand, within AMB_RS23465 at 2975.673 kb on + strand, within AMB_RS23465 at 2975.674 kb on - strand, within AMB_RS23465 at 2975.878 kb on + strand, within AMB_RS23465 at 2975.879 kb on - strand, within AMB_RS23465 at 2975.910 kb on + strand at 2975.910 kb on + strand at 2976.097 kb on + strand at 2976.097 kb on + strand at 2976.098 kb on - strand at 2976.098 kb on - strand at 2976.162 kb on + strand at 2976.162 kb on + strand at 2976.410 kb on + strand at 2976.411 kb on - strand at 2976.459 kb on + strand, within AMB_RS14025 at 2976.473 kb on + strand, within AMB_RS14025 at 2976.474 kb on - strand, within AMB_RS14025 at 2976.507 kb on + strand at 2976.508 kb on - strand at 2976.576 kb on + strand at 2976.775 kb on - strand, within AMB_RS14030 at 2976.889 kb on - strand at 2976.890 kb on + strand at 2976.927 kb on + strand at 2977.085 kb on + strand at 2977.085 kb on + strand at 2977.086 kb on - strand at 2977.086 kb on - strand at 2977.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,975,521 + AMB_RS23465 0.72 -0.0 2,975,521 + AMB_RS23465 0.72 -0.6 2,975,577 + AMB_RS23465 0.75 -0.1 2,975,577 + AMB_RS23465 0.75 +0.1 2,975,578 - AMB_RS23465 0.75 -2.2 2,975,578 - AMB_RS23465 0.75 -1.8 2,975,673 + AMB_RS23465 0.79 -1.4 2,975,674 - AMB_RS23465 0.80 -0.1 2,975,878 + AMB_RS23465 0.89 -1.8 2,975,879 - AMB_RS23465 0.89 +0.5 2,975,910 + -0.3 2,975,910 + -0.1 2,976,097 + -1.8 2,976,097 + +1.9 2,976,098 - +0.3 2,976,098 - +0.1 2,976,162 + +1.4 2,976,162 + -0.2 2,976,410 + -0.0 2,976,411 - -0.4 2,976,459 + AMB_RS14025 0.54 +0.2 2,976,473 + AMB_RS14025 0.72 +0.3 2,976,474 - AMB_RS14025 0.74 -1.2 2,976,507 + -0.5 2,976,508 - -0.1 2,976,576 + -1.2 2,976,775 - AMB_RS14030 0.65 +0.4 2,976,889 - +0.4 2,976,890 + -0.9 2,976,927 + -0.3 2,977,085 + -1.9 2,977,085 + -0.9 2,977,086 - +0.4 2,977,086 - +0.3 2,977,086 - +0.5
Or see this region's nucleotide sequence