Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12610 and AMB_RS12615 are separated by 124 nucleotides AMB_RS12615 and AMB_RS12620 are separated by 209 nucleotides
AMB_RS12610: AMB_RS12610 - bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA, at 2,686,608 to 2,689,727
_RS12610
AMB_RS12615: AMB_RS12615 - phytoene/squalene synthetase, at 2,689,852 to 2,690,589
_RS12615
AMB_RS12620: AMB_RS12620 - squalene synthase HpnC, at 2,690,799 to 2,691,629
_RS12620
Position (kb)
2689
2690
2691 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2688.886 kb on + strand, within AMB_RS12610 at 2689.185 kb on - strand, within AMB_RS12610 at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.524 kb on - strand at 2689.524 kb on - strand at 2689.524 kb on - strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.528 kb on - strand at 2689.528 kb on - strand at 2689.622 kb on + strand at 2689.730 kb on + strand at 2689.730 kb on + strand at 2689.753 kb on + strand at 2689.962 kb on - strand, within AMB_RS12615 at 2690.229 kb on + strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.318 kb on + strand, within AMB_RS12615 at 2690.318 kb on + strand, within AMB_RS12615 at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.580 kb on - strand at 2690.580 kb on - strand at 2690.580 kb on - strand at 2690.580 kb on - strand at 2690.753 kb on + strand at 2690.753 kb on + strand at 2690.753 kb on + strand at 2690.753 kb on + strand at 2690.753 kb on + strand at 2690.753 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.755 kb on + strand at 2690.757 kb on + strand at 2690.757 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,688,886 + AMB_RS12610 0.73 +0.4 2,689,185 - AMB_RS12610 0.83 -1.4 2,689,523 + +0.2 2,689,523 + +0.1 2,689,523 + -1.2 2,689,523 + -0.2 2,689,524 - -1.4 2,689,524 - +0.2 2,689,524 - +1.4 2,689,527 + -0.0 2,689,527 + -2.1 2,689,527 + +0.6 2,689,527 + -0.6 2,689,527 + -0.2 2,689,528 - -1.0 2,689,528 - -0.1 2,689,622 + +0.1 2,689,730 + -2.1 2,689,730 + -1.8 2,689,753 + -0.7 2,689,962 - AMB_RS12615 0.15 -2.0 2,690,229 + AMB_RS12615 0.51 -1.0 2,690,230 - AMB_RS12615 0.51 -1.3 2,690,230 - AMB_RS12615 0.51 -0.8 2,690,230 - AMB_RS12615 0.51 -0.8 2,690,230 - AMB_RS12615 0.51 -0.6 2,690,318 + AMB_RS12615 0.63 -2.8 2,690,318 + AMB_RS12615 0.63 -0.6 2,690,579 + -0.7 2,690,579 + +0.4 2,690,579 + -1.2 2,690,579 + -1.4 2,690,580 - -1.0 2,690,580 - +0.2 2,690,580 - -1.4 2,690,580 - -0.8 2,690,753 + -1.2 2,690,753 + -1.8 2,690,753 + -3.0 2,690,753 + -1.0 2,690,753 + -1.4 2,690,753 + -0.3 2,690,755 + +0.4 2,690,755 + -2.6 2,690,755 + +0.1 2,690,755 + -1.8 2,690,755 + -1.0 2,690,755 + -3.2 2,690,755 + -0.4 2,690,755 + +0.3 2,690,757 + -1.0 2,690,757 + -0.1
Or see this region's nucleotide sequence