Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12595 and AMB_RS12600 are separated by 114 nucleotides AMB_RS12600 and AMB_RS12605 overlap by 1 nucleotides AMB_RS12605 and AMB_RS12610 are separated by 26 nucleotides AMB_RS12610 and AMB_RS12615 are separated by 124 nucleotides
AMB_RS12595: AMB_RS12595 - cytochrome c, at 2,685,329 to 2,685,769
_RS12595
AMB_RS12600: AMB_RS12600 - amino acid-binding protein, at 2,685,884 to 2,686,396
_RS12600
AMB_RS12605: AMB_RS12605 - DUF2892 domain-containing protein, at 2,686,396 to 2,686,581
_RS12605
AMB_RS12610: AMB_RS12610 - bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA, at 2,686,608 to 2,689,727
_RS12610
AMB_RS12615: AMB_RS12615 - phytoene/squalene synthetase, at 2,689,852 to 2,690,589
_RS12615
Position (kb)
2686
2687
2688
2689
2690 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2685.642 kb on + strand, within AMB_RS12595 at 2685.642 kb on + strand, within AMB_RS12595 at 2685.642 kb on + strand, within AMB_RS12595 at 2685.642 kb on + strand, within AMB_RS12595 at 2685.642 kb on + strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.643 kb on - strand, within AMB_RS12595 at 2685.796 kb on + strand at 2685.797 kb on - strand at 2685.798 kb on + strand at 2685.798 kb on + strand at 2685.799 kb on - strand at 2685.889 kb on + strand at 2685.907 kb on - strand at 2685.907 kb on - strand at 2685.922 kb on + strand at 2686.287 kb on - strand, within AMB_RS12600 at 2686.546 kb on + strand, within AMB_RS12605 at 2686.547 kb on - strand, within AMB_RS12605 at 2686.579 kb on + strand at 2686.616 kb on + strand at 2686.824 kb on + strand at 2687.430 kb on - strand, within AMB_RS12610 at 2687.572 kb on - strand, within AMB_RS12610 at 2687.664 kb on + strand, within AMB_RS12610 at 2687.665 kb on - strand, within AMB_RS12610 at 2687.751 kb on + strand, within AMB_RS12610 at 2687.751 kb on + strand, within AMB_RS12610 at 2687.825 kb on + strand, within AMB_RS12610 at 2687.908 kb on - strand, within AMB_RS12610 at 2687.943 kb on + strand, within AMB_RS12610 at 2687.943 kb on + strand, within AMB_RS12610 at 2687.943 kb on + strand, within AMB_RS12610 at 2687.944 kb on - strand, within AMB_RS12610 at 2687.979 kb on + strand, within AMB_RS12610 at 2687.980 kb on - strand, within AMB_RS12610 at 2687.980 kb on - strand, within AMB_RS12610 at 2688.556 kb on - strand, within AMB_RS12610 at 2688.886 kb on + strand, within AMB_RS12610 at 2689.185 kb on - strand, within AMB_RS12610 at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.523 kb on + strand at 2689.524 kb on - strand at 2689.524 kb on - strand at 2689.524 kb on - strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.527 kb on + strand at 2689.528 kb on - strand at 2689.528 kb on - strand at 2689.622 kb on + strand at 2689.730 kb on + strand at 2689.730 kb on + strand at 2689.753 kb on + strand at 2689.962 kb on - strand, within AMB_RS12615 at 2690.229 kb on + strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.230 kb on - strand, within AMB_RS12615 at 2690.318 kb on + strand, within AMB_RS12615 at 2690.318 kb on + strand, within AMB_RS12615 at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.579 kb on + strand at 2690.580 kb on - strand at 2690.580 kb on - strand at 2690.580 kb on - strand at 2690.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,685,642 + AMB_RS12595 0.71 -0.5 2,685,642 + AMB_RS12595 0.71 +0.4 2,685,642 + AMB_RS12595 0.71 -0.2 2,685,642 + AMB_RS12595 0.71 -2.1 2,685,642 + AMB_RS12595 0.71 +0.1 2,685,643 - AMB_RS12595 0.71 +0.1 2,685,643 - AMB_RS12595 0.71 -1.4 2,685,643 - AMB_RS12595 0.71 -0.2 2,685,643 - AMB_RS12595 0.71 -0.7 2,685,643 - AMB_RS12595 0.71 -0.4 2,685,643 - AMB_RS12595 0.71 -2.8 2,685,643 - AMB_RS12595 0.71 -0.2 2,685,643 - AMB_RS12595 0.71 +0.1 2,685,643 - AMB_RS12595 0.71 -0.8 2,685,796 + +0.2 2,685,797 - -1.6 2,685,798 + -0.0 2,685,798 + -0.1 2,685,799 - -0.1 2,685,889 + +0.1 2,685,907 - -0.6 2,685,907 - -0.3 2,685,922 + +0.0 2,686,287 - AMB_RS12600 0.79 -0.6 2,686,546 + AMB_RS12605 0.81 +0.1 2,686,547 - AMB_RS12605 0.81 +0.2 2,686,579 + -1.6 2,686,616 + -1.8 2,686,824 + -0.1 2,687,430 - AMB_RS12610 0.26 -0.6 2,687,572 - AMB_RS12610 0.31 +0.5 2,687,664 + AMB_RS12610 0.34 -2.0 2,687,665 - AMB_RS12610 0.34 -0.6 2,687,751 + AMB_RS12610 0.37 +0.8 2,687,751 + AMB_RS12610 0.37 -2.2 2,687,825 + AMB_RS12610 0.39 -0.3 2,687,908 - AMB_RS12610 0.42 -1.2 2,687,943 + AMB_RS12610 0.43 -0.3 2,687,943 + AMB_RS12610 0.43 -0.5 2,687,943 + AMB_RS12610 0.43 -0.3 2,687,944 - AMB_RS12610 0.43 -0.7 2,687,979 + AMB_RS12610 0.44 -2.9 2,687,980 - AMB_RS12610 0.44 -1.0 2,687,980 - AMB_RS12610 0.44 -1.0 2,688,556 - AMB_RS12610 0.62 +1.4 2,688,886 + AMB_RS12610 0.73 +0.4 2,689,185 - AMB_RS12610 0.83 -1.4 2,689,523 + +0.2 2,689,523 + +0.1 2,689,523 + -1.2 2,689,523 + -0.2 2,689,524 - -1.4 2,689,524 - +0.2 2,689,524 - +1.4 2,689,527 + -0.0 2,689,527 + -2.1 2,689,527 + +0.6 2,689,527 + -0.6 2,689,527 + -0.2 2,689,528 - -1.0 2,689,528 - -0.1 2,689,622 + +0.1 2,689,730 + -2.1 2,689,730 + -1.8 2,689,753 + -0.7 2,689,962 - AMB_RS12615 0.15 -2.0 2,690,229 + AMB_RS12615 0.51 -1.0 2,690,230 - AMB_RS12615 0.51 -1.3 2,690,230 - AMB_RS12615 0.51 -0.8 2,690,230 - AMB_RS12615 0.51 -0.8 2,690,230 - AMB_RS12615 0.51 -0.6 2,690,318 + AMB_RS12615 0.63 -2.8 2,690,318 + AMB_RS12615 0.63 -0.6 2,690,579 + -0.7 2,690,579 + +0.4 2,690,579 + -1.2 2,690,579 + -1.4 2,690,580 - -1.0 2,690,580 - +0.2 2,690,580 - -1.4 2,690,580 - -0.8
Or see this region's nucleotide sequence