Experiment: 30_an_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11420 and AMB_RS11425 are separated by 4 nucleotides AMB_RS11425 and AMB_RS11430 overlap by 4 nucleotides AMB_RS11430 and AMB_RS11435 are separated by 10 nucleotides AMB_RS11435 and AMB_RS11440 overlap by 14 nucleotides
AMB_RS11420: AMB_RS11420 - signal peptidase I, at 2,461,065 to 2,461,808
_RS11420
AMB_RS11425: AMB_RS11425 - ribonuclease III, at 2,461,813 to 2,462,499
_RS11425
AMB_RS11430: AMB_RS11430 - GTPase Era, at 2,462,496 to 2,463,407
_RS11430
AMB_RS11435: AMB_RS11435 - hypothetical protein, at 2,463,418 to 2,463,633
_RS11435
AMB_RS11440: AMB_RS11440 - DNA repair protein RecO, at 2,463,620 to 2,464,341
_RS11440
Position (kb)
2462
2463
2464 Strain fitness (log2 ratio)
-2
-1
0
1 at 2461.939 kb on + strand, within AMB_RS11425 at 2462.345 kb on - strand, within AMB_RS11425 at 2462.414 kb on + strand, within AMB_RS11425 at 2462.414 kb on + strand, within AMB_RS11425 at 2462.414 kb on + strand, within AMB_RS11425 at 2463.207 kb on + strand, within AMB_RS11430 at 2463.375 kb on + strand at 2464.009 kb on + strand, within AMB_RS11440 at 2464.009 kb on + strand, within AMB_RS11440 at 2464.009 kb on + strand, within AMB_RS11440 at 2464.009 kb on + strand, within AMB_RS11440 at 2464.009 kb on + strand, within AMB_RS11440 at 2464.010 kb on - strand, within AMB_RS11440 at 2464.010 kb on - strand, within AMB_RS11440 at 2464.010 kb on - strand, within AMB_RS11440
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_3 remove 2,461,939 + AMB_RS11425 0.18 -1.0 2,462,345 - AMB_RS11425 0.77 -2.6 2,462,414 + AMB_RS11425 0.87 +0.7 2,462,414 + AMB_RS11425 0.87 +0.8 2,462,414 + AMB_RS11425 0.87 -2.0 2,463,207 + AMB_RS11430 0.78 -1.6 2,463,375 + -1.0 2,464,009 + AMB_RS11440 0.54 -1.6 2,464,009 + AMB_RS11440 0.54 -0.9 2,464,009 + AMB_RS11440 0.54 +0.1 2,464,009 + AMB_RS11440 0.54 -1.0 2,464,009 + AMB_RS11440 0.54 +0.4 2,464,010 - AMB_RS11440 0.54 -2.6 2,464,010 - AMB_RS11440 0.54 -0.4 2,464,010 - AMB_RS11440 0.54 -0.7
Or see this region's nucleotide sequence